Yeast in Biofuel
Yeast in Biofuel Overview
In general, yeasts used in the biofuel industry are in complex medium such as corn mash, corn stover, or sugar cane, which can serve as nutrients to produce bioethanol. These complex media increase the difficulties for automated counting using bright field method due to the debris particles in the sample. Using a combination of acridine orange (AO) and propidium iodide (PI) can perform dual fluorescence detection of live and dead cells, respectively, which can specifically stain the live/dead cells to accurately determine the concentration and viability of the complex sample.
Fluorescence Based Image Cytometry Method for Yeast Concentration & Viability Measurement
1. Pipette 20 µl of sample
2. Insert slide
3. Click count and get results
How Does Yeast Count & Viability by Dual Fluorescence Work?
Yeasts Used in Biofuel Industry
Yeast Concentration & Viability Measurement
Below shows 3 complex samples of corn mash, corn stover, and sugar cane. The bright field images contain many debris particles, but in the fluorescent images, only the yeast cells that fluoresce brightly will be automatically counted.
Corn Mash Debris
Corn Stover Debris
Sugar Cane Debris
Measuring Yeast Viability Using AOPI
Identification of live and dead cells in a complex yeast sample