NCI-60 Cancer Cell Lines

Introduction to NCI-60 Human Cancer Cell Lines

Nexcelom performed Cellometer image cytometry on all NCI-60 cancer cell lines. Every cell sample was individually stained with trypan blue, imaged and analyzed using the Cellometer Auto T4. Each sample was assessed for viability and cell size. The ability to accurately measure cell viability and cell size are two important components in determining the functionality of anticancer compounds. Cell death, or reduced cell size in certain tumor cell lines could be an indicator of compound efficacy.

NCI-60 Human Cancer Cell Lines

Using the Cellometer Auto T4, we imaged and counted every NCI-60 cancer cell line, obtaining the cell concentration, viability, and cell size. Shown here, are the enlarged representative images of each cell line. Since all of the cells were stained with trypan blue, we show the stained trypan blue image as well as the Cellometer counted image.

The cells are organized alphabetically by the organ or origin.

Download Excel Table: Cell size distribution for all 60 NCI cancer cell lines

NCI-60 Breast Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 breast cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Central Nervous System Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 central nervous system cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Colon Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 colon cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Kidney Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 kidney cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Leukemia Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 leukemia cell lines.. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Lung Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 lung cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Melanoma Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 melanoma cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Ovarian Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 ovarian cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

NCI-60 Prostate Cell Lines

Shown below are stained trypan blue images and Cellometer counted images of NCI-60 prostate cell lines. Counted live cells are outlined in green while the dead trypan blue positive cells are outlined in red.

List and Description of NCI-60 Cancer Cell Lines

Compiled data from multiple sources ^{1, 2, 3}

Download the full NCI-60 cancer cell line data list, which includes the cell size measurements of all 60 cell lines.

Conclusions

The Cellometer line of instruments is capable of measuring the cell concentration, viability, and cell size of all 60 NCI cancer cell lines. The innovative “Accurate Cell Membrane Outline Algorithm” assures that all cell samples are de-clustered and counted properly. The ability of the Cellometer instruments to accurately and consistently measure cell concentration, viability, and cell size provides researchers with a reliable tool in their cancer research.

Publications Using Cellometer Counting for NCI-60 Cancer Cell Lines

• Tedeschi PM, Markert EK, Gounder M, et al. (2013) Contribution of serine, folate and glycine metabolism to the ATP, NADPH and purine requirements of cancer cells. Cell Death & Disease 4:e877.
• Dolfi SC, Chan LL, Qiu J, et al. (2013) The metabolic demands of cancer cells are coupled to their size and protein synthesis rates. Cancer & Metabolism 1(1):20.

Additional Citations

1. Jerry M. Collins, Developmental Therapeutics Program NCI/NIH. http://dtp.nci.nih.gov/
2. DTP, DCTD Tumor Repository. A Catalog of in Vitro Cell Lines, Transplantable Animal and Human Tumors and Yeast. The Division of Cancer Treatment and Diagnosis (DCTD), National Cancer Institute. 2013
3. Abaan OD, Polley EC, Davis SR, et al. (2013) The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Research.