Cellometer X2 Fluorescent Viability Counter
Fluorescent automated cell counter for yeast, platelets and small cells
Introduction to the Cellometer X2 Fluorescent Viability Counter
Automated Fluorescent Yeast and Small Cell Counting in <60 Seconds
Cell Counting Made Simple
The Cellometer X2 utilizes bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
5x Faster than Manual Counting: Load sample, view image, count cells, and obtain results in < 60 seconds
The Cellometer X2 Allows Hemacytometer Users to:
- Increase throughput
- Improve consistency
- Ensure all data is correctly captured
- Count difficult cells (clumpy, irregular-shaped)
- Eliminate judgment errors, miscounts, and user-to-user variability
Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer X2.
Because mature mammalian red blood cells don’t contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.
Fluorescent Viability Determination
Use of a fluorescent dye, such as propidium iodide (PI), is highly recommended for accurate viability analysis of cell samples containing debris.
Propidium iodide (PI) is a nuclear staining (nucleic acid binding) dye that enters dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence.
Because the Cellometer X2 can count dead cells in the red fluorescence channel, there is no interference from debris and live cells.
With propidium iodide (PI), dead cells are clearly visible in the red fluorescence channel.
1-Step Yeast Concentration & Viability Analysis using Cellometer X2
Bright field and Fluorescent Imaging
Bright field and fluorescent images of yeast cells. Cells appearing in both the bright field and fluorescent image are dead.
The Cellometer X2 Image Cytometer is specifically optimized for simple, 1-step determination of yeast concentration and viability. The Cellometer X2 is ideal for research laboratories and small and large breweries looking to automate their fermentation monitoring. Performance of the Cellometer X2 has been proven in the largest breweries in the U.S.
Bright field images can be viewed to confirm cell morphology. Counted bright field images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both bright field and fluorescent images from each sample makes 1-step concentration & viability determination possible. Read more about yeast viability.
Yeast Concentration Measurement by Bright Field Analysis
Single Cell Count
De-clustering of Yeast Cells
Chain-Forming Cell Count
Yeast Concentration & Viability Measurement by Bright Field & Fluorescence
Viability Measurement Using Propidium Iodide (PI)
Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.
Viability Measurement by Oxonol
Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.
Yeast Concentration Measurement by Dual Fluorescence Detection
Yeast Viability by Dual-Fluorescence
Yeast samples are stained 1-to-1 with a mixture of acridine orange (AO) and propidium iodide (PI) dual-fluorescence stain. Yeast concentration and viability are obtained immediately after staining using Cellometer X2. Live yeast cells fluoresce green and dead cells fluoresce red.
Yeast Vitality by Fluorescent Enzymatic Stain
Yeast samples are stained 1-to-1 with Carboxyfluorescein-AM fluorescent enzymatic stain for 45 minutes and then analyzed for vitality using Cellometer X2. Bright field images are used for total cell count and fluorescent images are used to measure the active yeast cells.
Yeast Cell Cycle Analysis Using Propidium Iodide (PI)
Standard baker’s yeast stained with the Cell Cycle Staining Kit from Nexcelom Bioscience are incubated for 60 minutes before using Cellometer X2 to analyze the cell cycle. The plot shows the yeast population that is actively dividing. Their higher DNA content is measured using PI.
Automated Platelet Counting in Whole Blood
Fluorescence-Based Platelet Concentration. Measurement A blood sample stained using the Calcein AM Vitality / Viability Kit from Nexcelom Bioscience is incubated for 20 minutes. Both platelets and white blood cells produce green fluorescence. Cell size gating is applied to exclusively count platelets.
Exclusion of Debris & Size-Based Counting
Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.
- Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
- A fluorescent nuclear dye, such as acridine orange, can be used to more easily identify nucleated cells in samples containing debris.
The Cellometer X2 Image Cytometer automatically generates a cell size histogram based on cell diameter.
The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.
View, Print, and Save Cell Images and Data Tables
Bright field and fluorescent counted images of mouse splenocytes stained with acridine orange.
View bright field images to check cell morphology.
View fluorescent counted images to confirm exclusion of debris or unwanted cell types and correct counting of cells within clumps.
Easily Export to Excel for additional data formatting or sharing. Images and data tables can be pasted into PowerPoint presentations or submitted for publication.
Easily Save data and images to a network or local computer.
Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images.
Cellometer X1 vs. X2
|Cellometer Yeast Analysis||Cellometer X1||Cellometer X2|
|Bright field imaging mode||X||X|
|Single fluorescence imaging mode||X||X|
|Dual fluorescence imaging mode||X|
|Viability using PI||X||X|
|Concentration & viability using AOPI||X|
|Vitality using CFDA-AM||X|
|Yeast cell cycle||X||X|
|Cellometer software for analysis of clumpy and
|Mean diameter and cell size distribution||X||X|
|Cell type wizard for creating new cell type parameters||X||X|
Counting Chambers: No Washing or Contamination
Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette.
The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.
The disposable Cellometer Cell Counting Chambers offer several key advantages:
- Time savings – no washing
- No risk of cross-contamination
- Reduced biohazard risk to users
- Controlled sample volume
- Most affordable automated counting consumables
Dedicated On-line and On-site Applications Support
Clicking “Submit a Support Ticket” launches a convenient online form that is submitted directly to Nexcelom Technical Support
- X2 instrument information fields are automatically populated
- Images are easily attached for troubleshooting and applications support
- A Nexcelom Specialist can assume remote control of the Cellometer X2 Image Cytometer for troubleshooting and training
Applications for Cellometer X2 Fluorescent Viability Counter
Performance of the Cellometer X2 Image Cytometer
Dynamic Range of Total Cells with Cellometer X2
Concentration Dynamic Range Figure 1 depicts the dynamic range for cell concentration measured by Cellometer X2. This data set was taken on a concentration series of cultured yeasts.
Samples from 2.5 x 105 – 5 x 107 cells/ml can be counted without further dilution.
The %CV at each concentration was below 10%.
Figure 1: Table of results for cell concentration dynamic range
Cellometer X2 Repeatability and Consistency
|Cellometer X2||Average Live Cell Concentration Via Fluorescence||Viability|
Figure 2: Table of results for cell concentration and viability using acridine orange (AO) and propidium Iodide (PI)
The results indicate the accuracy of the Cellometer X2 instrument in assessing the viability of yeasts using AOPI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X2 in measuring cell concentration and viability of yeast cells.
Performance of the Cellometer X1 Image Cytometer
Dynamic Range of Total Cells with Cellometer X1
Concentration Dynamic Range Figure 3 depicts the dynamic range for cell concentration measured by Cellometer X1. This data set was taken on a concentration series of cultured yeasts.
Samples from 4 x 105 – 5 x 107 cells/ml can be counted without further dilution.
The %CV at each concentration was below 10%.
Figure 3: Table of results for cell concentration dynamic range
Cellometer X1 Repeatability and Consistency
|Cellometer X1||Average Live Cell Concentration Via Bright Field||Viability|
Figure 4: Table of results for cell concentration and viability using bright-field and Propidium iodide (PI)
The results indicate the accuracy of the Cellometer X1 instrument in assessing the viability of yeasts using PI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X1 in measuring cell concentration and viability of yeast cells.
Cellometer X1 and X2 Consistency and Accuracy Comparison to Hemacytometer
5 µm beads
|N = 20||Hemacytometer||Cellometer X1 and X2|
Our Cellometer X2 cell counter makes counting cells easier and less time consuming! Also, the unit is reliable and needs little to no maintenance. It just always works well!
The Cellometer X2 is very fast and easy to use! It has saved a ton of time versus counting by hand. Training new people is a breeze, too. Nexcelom’s support team has been extremely helpful with any questions or issues as well!
Our Cellometer X2 has made cell counting quicker, faster and easier by allowing us to perform more cell counts and improve our pitch rates at the brewery!
Our lab has purchased the Cellometer X2. We found that counting yeast cell cultures is very fast and accurate, especially since we can change the parameters. For example, we can change cell counts based on cell size. Also, there is an option of declustering which is exceptionally helpful for yeast cultures that are arrested at the G2 stage of the cell cycle, which is necessary for many of our experiments. – Cynthia Sakofsky, NIEHS
We are very happy with our new Cellometer X2! We are pleasantly surprised to find the accompanying laptop already loaded with the necessary software upon purchasing it. Nexcelom customer service has been excellent as well!
We have four Cellometer X2 units, all in different labs and locations. I trust that they give us comparable results, which is invaluable for ensuring consistency of our fermentations and products across locations!
Our Cellometer X2 cell counter makes counting cells easier and less time consuming! Also, the unit is reliable and needs little to no maintenance. It just always works well! – Sarah DeLorenze, Two Roads Brewing Company
Our Applications Scientist helped establish mammalian cell counting protocols for the Cellometer X2 as well as the Cellometer Auto 2000. Both instruments have made cell counting amazingly simple and accurate for live dead cells. We appreciate the training!
The Cellometer X2 is working great to count beads size 3-5 microns. We are also using it to count beads that are 50um in diameter and the numbers are extremely accurate. Our local Applications Scientist from Nexcelom was very thorough for the training process and really helpful in establishing relevant protocols.
Our team likes the ease of use that the Cellometer X2 offers us! Our cell counts are able to be completed clearly and efficiently.
Our local Applications Scientist from Nexcelom did a great job of setting up our new Cellometer X2! He showed us the AOPI dye method – which we have never used before. We now look forward to using this due for more accurate assays and optimizing the SOP to suit our needs. Thanks Nexcelom!
The Cellometer X2 is very has fast and easy to use! It has saved a ton of time versus counting by hand. Training new people is a breeze, too. Nexcelom’s support team has been extremely helpful with any questions or issues as well! – Robin Mead, Oskar Blues Brewery
Our Cellometer X2 has made cell counting quicker, faster and easier by allowing us to perform more cell counts and improve our pitch rates at the brewery! – Molly Eveleth, Swamp Head Brewery
The Cellometer X2 is the perfect instrument for your daily cell counting needs. It is very easy to operate so training others and passing along knowledge is a breeze. While the images are very useful and give us precise counts, they also allowing us to see how our yeast is flocculating. – Alec Dvoretsky, Two Roads Brewing Company
The Cellometer X2 device has made cell counting and viability assessment a breeze! In a production brewery environment where accurate yeast cell counts and viabilities are essential to creating the perfect beer, the Cellometer X2 system makes the process simple and accurate without complex training or additional equipment. Compared to the traditional microscope and hemocytometer, the X2 system and software are easy to use, easy to interpret and simple to adjust. I would recommend this system to any brewery! – Tom Boudreau, Stone Brewing
The Cellometer X2 is an easy to use, reliable cell counter. Made our life in the lab way easier! We are very happy with our Cellometer X2! – Veronique Grenier, Maui Brewing Co.
We have just received our brand new Cellometer X2! We are looking forward to seeing how we can manage fermentation using the X2!
The Cellometer X2 is an oustanding machine for applications. We love it and use it often! It is user friendly and easy to use for the students. We also have published data with the machine.- Sumi Dinda, Oakland University
The Cellometer X2 has significantly improved our proccess for enumerating our cultures without having to wait 2-3 days for plates to grow. Along with the yeast viability dye, we are able to get both concentration and viability of our cultures in less than an hour, including performing dilutions!
I like how the images can be saved for reference on the Cellometer X2! – Raymond Furman, MGPI of Indiana, LLC
We use the Cellometer X2 to develop assays to measure protozoa and bacterial cell counts.
The Cellometer X2 has provided me with reliable counting that has standardized our cytotoxicity assay and maintains consistency across various users. Previously, I was using a hemacytometer for counting our human cell lines, which would often give me intense headaches from staring into a microscope for long periods of time. This is no longer a problem with the Cellometer X2! I would reccommend this product to anyone who needs reliable cell counts for mammalian cell lines.
The Cellometer X2 has increased the throughput for my mammalian cell counting applications tremendously. Before, I was using a hemacytometer for counting cells, which was very time consuming, and it gave me a headache! But now, I can count my cells quickly, which gives me more time to do other tasks. Thanks Nexcelom!
Our new Cellometer X2 has cut the time we spend on cell counts drastically, freeing up time for much more. The transition was very easy, the software is a piece of cake to learn and the support is excellent! -Josh Weaver, Westbrook Brewing Company
The Cellometer X1 is easy to use, accurate and fast. And to perform viability and total cell count in 1 step is fantastic. –Jim Elliott, Captain Lawrence Brewing
The Cellometer X2 has made our daily cell counts and cellar reports much faster and easier. The software interface and sample prep is easy to perform and give consistent, reliable data. – Joe Wells, Hangar 24
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