///Customer Publications – Celigo
Customer Publications – Celigo 2018-06-01T17:46:36+00:00

Publications Referencing Celigo

Below is a list of peer reviwed journals, such as Nature, Breast Cancer Research and Journal of Cell Biology, that reference Celigo imaging cytometer.

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Celigo Product Guide
Author Date Title Journal Description of Cells Description of how Celigo was used
Dominguez, Charli November 2017 Neutralization of IL-8 decreases
tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative
breast cancer
JCI insight MDA-MB-231, BT549, and Effector
NK cells.
Celigo was used to determine the
cell viability after staining with propidium iodide
Kwang Ha, Tae November 2017 Baicalein Reduces Oxidative
Stress in CHO Cell Cultures and Improves Recombinant Antibody
Productivity
Biotechnology Journal rCHO cells Celigo was used to analyze ROS
level of cells stained with CM-H2DCFDA.
Xu, S November 2017 Basic Transcription Factor 3 Is
Involved in Lung Cancer Growth and Progression Supplement: Supplement
Journal of Thoracic Oncology Human NSCLC cells NCI-H1299 and A549 Human NSCLC NCI-H1229 and A549
cells were transfected with BFTF3 shRNA lentiviral vector. Celigo was used to analyze cell
proliferation, cell death, and viability of human NSCLC
H, Liu November 2017 AK2 Is Overexpressed and
Correlated with Increased Metastasis Potential and Tumorigenicity in Lung
Adenocarcinoma: Supplement
Journal of Thoracic Oncology LAD cells Celigo cell imaging analyzer,
flow cytometric analysis, cell transwell assays, Immunofluorescence
test and western blotting were performed to assess the effects of AK2 on
proliferation, apoptosis,
cell cycle, autophagy and epithelial-to-mesenchymal
(EMT) phenotypes in LAD cells in vitro.
Pan, Kung November 2017 ERH is up-regulated in bladder
cancer and regulates the proliferation and apoptosis of T24 bladder cancer
cells
Journal of Thoracic Oncology bladder cancer cell lines T24 and 5637 BrdU incorporation and
Celigo assays were
used to examine the cell proliferation of ERHshRNA
or scrambled-shRNA cell lines
Dame, Keri November 2017 Derivation of Thyroid
Progenitors from Embryonic Stem Cells through Transient, Developmental
Stage-Specific Overexpression of Nkx2-1
ProQuest Dissertations Publishing Thyroid cells are derived from mouse embryonic stem cells  
Filipovic, Nenad November 2017 Selenotriapine An isostere of
the most studied thiosemicarbazone with pronounced pro-apoptotic activity,
low toxicity and ability to challenge phenotype reprogramming of 3-D mammary
adenocarcinoma tumors
Arabian Journal of Chemistry MCF-7 tumor models The Celigo was used to measure
the area of the spheroids. Measurments were taken over an 8 day period and
spheroid size was compared to size on day 0. Growth of treated and untreated
spheroids were measured.
Hanigan, Thomas October 2017 Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and posttranslational modification state of class I HDACs Plos One MCF-7 cells MCF-7 cells were fixed with methanol and stained with PI. The Celigo was used to image the cells andperform cell cycle analysis.
Quentin Li, Qingdi October 2017 Protein kinase D inhibitor
CRT0066101 suppresses bladder cancer growth in vitro and xenografts via
blockade of the cell cycle at G2/M
Cellular and Molecular Life Sciences Bladder cancer cells: T24, T24T, TCCSUP, and UMUC1. Human uroepithelial cells: SV-HUC Bladder cancer and human
uroepithelial cells were incubated with a compound called CRT0066101. After
an incubation period, the cells were stained with calcein AM, PI, and
Hoechst. Celigo was used to capture images of the cells and determine the
viability.
Ortiz-Virumbrales, Maitane October 2017 CRISPR/Cas9-Correctable
mutation-related molecular and physiological phenotypes in iPSC-derived
Alzheimer’s PSEN2N141I neurons
Acta Neuropathologica Communications 7889(s)B, 050643 (Control), 948 (AD1), 949(fControl), and 950 (AD2) iPSC lines Celigo was used to capture
images and analyze iPSC cell lines stained with PI.
Hsu, Chih-Jung October 2017 Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system Biotechnology and Applied Biochemistry HeLa cells The Celigo was used to analyze the “the
transfection efficiency of each dTtaPS-plasmid complex”. Celigo captured images of GFP positive cells to determine the fluorescence intensity in each well.
Zhuang, Peng-Yuan September 2017 Effect of TALEN-mediated IL-6 knockout on cell proliferation,
apoptosis, invasion and anti-cancer therapy in hepatocellular
carcinoma (HCC-LM3) cells
Oncotarget HCCLM3-wt or HCCLM3-IL6(-) cells HCCLM3-wt or HCCLM3-IL6(-) were
treated with sorafenib or IFN_. To determine the proliferation cells, cell
counting kit-8 was added to the 96-well plates. Celigo was used to determine
the absorbance of cells at 490 nm.
Shaw, David September 2017 Development and Characterization of an Automated Imaging Workflow to Generate Clonally-Derived Cell Lines for Therapeutic Proteins Biotechnology Progress CHO-K1 Cells The Celigo was used to count CHO-K1 cells stained with CellTracker Green CMFDA 0 days and 21 days after plating.” Both CHO-K1 Green and CHO-K1. Red expressing CHO-K1 hosts were transfected with the same DNA preparation
of mAb B” and plated into 384-well plates after electroporation. After the plates were imaged, the results were used to ” drive automated hit-picking where 1,056 wells with growth were picked at random into 96-well plates.”
Tsuboi, Setskuko September 2017 Critical Review—Water-Soluble Near-Infrared Fluorophores Emitting over 1000 nm and Their Application to In Vivo Imaging in the Second Optical Window (1000–1400 nm) ECS Journal of Solid State Science and Technology HeLa cells In order to determine the toxicity of NIR fluorophores, the Celigo was used to determine the number of HeLa cells 0-70 hours after treatment with NIR fluorophoes.
Li, Wei September 2017 RDM1 gene Overexpression Represents a Therapeutic Target in Papillary Thyroid Carcinoma Running title: The new therapeutic target of papillary thyroid carcinoma Endocrine Connections K1 and TPC1 cells K1 and TPC1 cells were stained post lentivirus transduction to determine the rate of siRNA gene knockdown. 96-well plates were scanned on the Celigo using bright field and the green fluorescent channel. After gating, the Celigo determined the total cell count and ” the average integrated red fluorescence intensity”. The Celigo was used to create a scatter plot of “green fluorescence area (in µm2) and integrated fluorescence intensity.
Zhour, Yizhou September 2017 Beating the Odds: The Poisson Distribution of All Input Cells During Limiting Dilution Grossly Underestimates Whether a Cell Line is Clonally-Derived or Not Biotechnology Progress CHO-K1 cells The Celigo was used to image plates initially and three weeks later to determine the distribution of “mAbX expressing CHO-K1 cells [that] were transfected with
either mAbX-sp1 or mAbX-sp2 vectors”. 384-well plates were imaged in bright field and fluorescent channels.
Kessel, Sarah September 2017 Real-Time Apoptosis and Viability HighThroughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer SLAS Discovery MCTS produced from the glioblastoma cell line U87MG The Celigo was used to examine the “the kinetic apoptotic and cytotoxic effects” of numerous compounds of multicellular tumor spheroids. The bright field channel, blue channel, red channel, green channel, and far red channel was used on the Celigo to image and analyze the plates.
Miao, Ruoyu September 2017 Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer. Scientific Reports Liver Cancer Cells The Celigo was used to perform in situ cellular analysis on liver cancer cells after TTK inhibition. They also used Annexin V-FITC/PI Hoechst Apoptosis assay on the Celigo. They performed cell cycle analysis using BrdU and DAPI stains on the Celigo. Lastly, they used the Celigo to capture bright-field images of cell growth.
Mi Gu, Sun August 2017 A synthetic cannabinoid JWH-210 reduces lymphoid organ weights and T-cell activator levels in mice via CB2 receptors Naunyn-Schmiedeberg’s Arch Pharmacol Splenocytes and Thrymocytes The Celigo was used to measure the cell viability of splenocytes after exposing cells to EthD-1 and calcein AM. It was also used to measure fluorescent intensity of splenocytes or thymocytes stained with DAPI and “secondary antibodies conjugated to AlexaFluor 488 and 594”
Briffa, Romina August 2017 Unravelling the role of TRIB1 in colorectal cancer: a functional molecular pathology approach EPMA Journal three stable TRIB1 knock-down CRC cell line The Celigo was used to monitor “cell cycle progression, proliferation, tumour growth, and invasion”.
Tomita, Kyoko August 2017 Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis JCI insight BMDM or THP-1 cells The Celgio was used to determine the quantity of migrated BMDMs and THP-1 cells that were fluorescently labeled.
He, Xuefeng July 2017 Opa interacting protein 5 acts as an oncogene in bladder cancer Journal of Cancer Research Clinical Oncology BC-T24 and BC-5637 The Celigo was used to monitor cell growth of BC-T24 and BC-5637 after infection with shOIP5/shCtl. shOIP5 and shCtl fluorescence intensity was monitored using the Celigo and the cell counts were determined from the measured fluorescence intensity. This was to determine the “knockdown of shOIP5 suppressed cell growth”.
Leonidou, Andri July 2017 Identification and Validation of Driver Kinases from Next-Generation Sequencing Data Kinase Signaling Networks    
Oliemuller, Erik July 2017 SOX11 promotes invasive growth and DCIS progression The Journal of Pathology MCF10A, MCF10DCIS.com, BT474, BT549, Cal148, HCC202, MX-1, and UACC893. Cells were grown in a ULA plate to allow spheroids to form. The Celigo was used to take images of the spheroids after four days. After fourteen days, the Celigo was used to determine the mammosphere-forming efficiency by determining the quantity of mamospheres and dividing by the number of cells plated per well. The Celigo was also used to measure the size of the spheroids and measure the fluorescence of Caspase-3.
Kessel, Sarah June 2017 TIReal-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image CytometerTLE Cytometry Part A. multicellular tumor spheroids The Celigo was used in combination with PI and caspase 3/7 stain to measure the rate of apoptosis and the viability of multicellular tumor spheroids
Daubeuf, François June 2017 A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse Current Protocols in Mouse Biology BAL cells, T cells, B cells, neutrophils, eosinophils, and macrophages  
Cherradi, S June 2017 Antibody targeting of claudin-1 as a potential colorectal cancer therapy Journal of Experimental Medicine & Clinical Cancer Research colorectal cancer cells The Celigo was used to read 6-well plates that contained colorectal cancer cells using the single colony verification application. The tumorsphere application was also used and captured images of the tumorspheres. Also, the expression analysis assay was used to determine the fluorescent signals. During this experiment, they stained the cells with DAPI nuclear stain. Lastly, the Celigo was used to determine the apoptosis and growth rate of the tumorspheres.
de Andrade Mello, Paola June 2017 Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death Oncotarget, Advance Publications MCA38 colon cancer cells The viability was determined after the cells were exposed to CCK-8 and the Celigo was used to capture images of the live cells.
Singh, Veena June 2017 Performance of Biocept’s sample collection for tumor cell analysis. Tumor Biology BT474 (HER2 amplified) and H3112 (ALK re-arranged) cells The Celigo was used to count BT474 (HER2 amplified) or H3112 (ALK re-arranged) cells after being thawed.
Nabbi, Arash May 2017 ING3 promotes prostate cancer growth by activating the androgen receptor BMC Medicine LNCaP, PC3, and DU145 cells The Celgio was used to monitor cell proliferation of LNCaP, PC3, and DU145 cells in a 96-well plate.
Kondo, Yasushi May 2017 Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells Diabetologia hiPSC lines: 648A1 and 692D2.
Fibroblast-derived hiPSC lines: 409B2, 206B6 and 201B7.
hESC lines: H9, KhES1 and KhES3.
Cells were immunostained with anti-insulin antibodies and the Celigo was used to determine the induction rate of INS+ cells.
Huang, Ying May 2017 Identification of hMex-3A and its effect on human bladder cancer cell proliferation Oncotarget Bladder cancer cells lines:5637 and T24 The Celigo was used to examine the proliferation after bladder cancer cells were transfected with an interference RNA sequence.
Grav, Lise May 2017 Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells Springer Link CHO Cells The Celigo was used to determine the cell survival and cell confluency.
Ivanov, Delyan May 2017 High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity Springer Link   References the Celigo as being capable of measuring spheroid size and volume in high throughput.
Chan, Leo May 2017 A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity Cytotherapy peripheral blood mononuclear cells (PBMC) “Viability was determined by employing different staining techniques such as enzymatic stain, nucleic acid stain, multi-stain method,
fluorescent stain, and trypan blue staining on the Celigo and Cellometer. “
Chan, Leo May 2017 Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry Springer Link   In this work, they “demonstrate a novel high-throughput cytotoxicity screening assay using the Celigo imaging-cytometry method”. A 96 well plate was used with the Celigo to analyze the percent lysis of cells.
Cuny, Gregory April 2017 PYRIMIDINE COMPOUNDS AND METHODS USING THE SAME United States Patent Application SH-SY5Y-hTAU441 cell The Celigo was used to assess the cytotoxicity of SH-SY5Y-hTAU441 cells.
Gheller, Brandon April 2017 PYY regulates human skeletal muscle progenitor cell proliferation The FASEB Journal Skeletal Muscle stem cells (satelitte cells) The Celigo was used to track percent confluence of cells over five days.
Blum, Jamie April 2017 Short-term Inflammation Increases Proliferative Capacity of Human Skeletal Muscle Progenitor Cells from Young and Old Female Donors The FASEB Journal Muslce Progenitor Cells The Celigo was used to track the percent confluence, number of nuclei, and percent cell death.
Smith, Paul April 2017 Microenviroment Cytometry Single Cell Analysis: Contemporary Research and Clinical Applications multicellular tumor spheroids (MCTSs) The Celigo was used to track the multicellular tumor spheroids health by staining with viability dye DRAQ7. They also looked at non-viable cells in the culture.
Zhang, Haohai April 2017 Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer Elsevier Chinease hamster ovary (CHO) cells “Demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression.”
Venugopal, Jessica April 2017 Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cells Elsevier kidney disease (ADPKD) cells The Celigo was used in combination with an inverted microscope to monitor the wound healing of a cell monolayer.
Yang, Mei-Lin March 2017 “IL-6 ameliorates acute lung injury in influenza virus infection” Scientific Reports fibroblasts isolated from IL-6 -/- mice and WT mice Fibroblasts were isolated from IL-6-/- mice and WT mice. The Celigo was used to determine the cell count, doubling time, and apoptosis rate by immunofluorescence with anti-cleaved caspase-3 antibody. It was also used to monitor the number of virus infected cells engulfed by macrophages compared to IL-6/- macrophages. The macrophages were exposed to QD649 particles and stained with DAPI.
Itoh, M March 2017 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture Technology Platforms for 3D Cell Culture: A User’s Guide   States how NCP can be used with the Celigo for measurments.
Slawny, Nicole March 2017 Physiologically relevant spheroid models for three‐dimensional cell culture Technology Platforms for 3D Cell Culture: A User’s Guide   Lists the Celigo as a type of automated 3D assay equipment
Quentin Li, Qingdi March 2017 Proteomic analysis of proteome and histone post-translational modifications in heat shock protein 90 inhibition-mediated bladder cancer therapeutics Scientific Reports 5637 bladder cancer cells They wanted to determine the antitumor impact of HSP90 inhibitors in 5637 bladder cancer cells. They stained the cells and used the Celigo to examine the cell viability.
Dall’Acqua, William January 2017 Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence Scientific Reports NCI-H358 non-small cancer cells Cell cytotoxicity studies were performed on a Celigo S Imaging cytometer. Overlay of brightfield with either green fluorescence or blue fluorescence identified and qualified CMFDA or BMQC positive cells using Celigo Image processing software.
Li, Linfeng January 2017 3D High-Content Screening of Organoids for Drug Discovery Elsevier None This paper listed the Celigo as a 3D high content screening system
Parker, Christopher January 2017 Ligand and Target Discovery byFragment-Based Screening in Human Cells Elsevier Human Cells  
Cribbes, Scott January 2017 A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer SAGE Journals glioblastoma cell line U87MG In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates.
Auberon, Florence December 2016 Isolation of novel stilbenoids from the roots of Cyrtopodium paniculatum (Orchidaceae) Fitoterapia human glioblastoma U-87MG cells Celigo
Braganza, Andrea December 2016 UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligase The Journal of Biological Chemistry human cells The cells were counted various times after plating. Colonies were also stained with Crystal violet dye and counted with the Celigo.
Boss, Olivier December 2016 Methods and compositions for inducing differentiation of human brown adiopocyte progenitors FPO adipose tissue (BAT) progenitor cells Cells were exposed to C1-BODIPY® 500/510 C12 (Molecular Probes #D-3823) and incubated for 3 to 6 hours. C1-BODIPY® 500/510 C12 is a fluorescent fatty acid analog that is sometimes utilized for lipid trafficking studies (ThermoFisher). Next, the cells were analyzed in bright field and fluorescence. They looked at the total fluorescent intensity per well.
Wu, Yao November 2016 Cucurbitacin-I induces hypertrophy in H9c2 cardiomyoblasts through activation of autophagy via MEK/ERK1/2 signaling pathway Toxicology Letters H9c2 cells The cell viabilities were detected by the Celigo Image Cytometer
Kramer, Daniela November 2016 Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells Cell Death & Differentiation human ovarian cancer cell lines  
Su, X November 2016 TAp63 suppresses mammary tumorigenesis through regulation of the Hippo pathway Oncogene mammary epithelial cells (MECs), mammary stem cells (MaSCs) and tumor-initiating cells (TICs)  
Wu, Yao November 2016 Cucurbitacin-I induceshypertrophy in H9c2 cardiomyoblasts through activation of autophagy viaMEK/ERK1/2 signaling pathway Toxicology Letters H9c2 cells The Celigo was used to analyze cell viability of H9c2 cells
Cisneros Castillo, Liliana October 2016 A novel computer-assisted approach to evaluate multicellular tumor spheroid invasion assay Scientific Reports tumor and peripheral cells To quantify the size of MCTS in invasion assays, the present of the Celigo Cytometer is an approach that comes already with a device that takes images automatically of an inserted plate.
Kari, Vijayalakshmi September 2016 Loss of CHD1 causes DNA repair defects and enhances prostate cancer therapeutic responsiveness The Embo Journal Prostate cancer cells  
Hamilton, Duane September 2016 Brachyury, a vaccine target, is overexpressed in triple- negative breast cancer Society for Endocrinology Triple-negative breast cancer cells Nuclei were stained using DAPI and images were acquired using the Celigo S cell Imaging Cytometer
Potapova, Tamara August 2016 Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53 Molecular Biology of the Cell Tetraplid cells Multi-well plates with labeled cells were imaged on Celigo Imaging Cytometer. Images were quantified and each image typically contained several hundred of cells; 3-4 images were analyzed to determine the mean number of positive cells and the standard deviation
Imamura, Yukio August 2016 Near-Infrared Emitting Pbs Quantum Dots for in Vivo Fluorescence imaging of the Thrombotic state in septic mouse brain Molecules HeLa cells HeLa cells were plated at 6000 cells/well in 96 well plates. After culturing overnight, QD1100 was added to every well at a final concentration of 0-50 nM. The number of cells in each well was counted with the Celgio after 0,7,24,4,8, and 60 hours.
Maguire, Sarah August 2016 3D modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model The Journal of Pathology MCF10A cells Comparison of pathway aberrations associated with progression identified that when cells are grown as 3D spheroids, they show perturbations of cancer-relevant pathways. The spheroids are was calculated using the Celigo S.
Chan, Leo Li-Yang August 2016 A high-throughput AO/PI- based cell concentration and viability detection method using the Celigo image cytometry Cytotechnology Jurkat cells The high-throughput AO/PI method described here allows for 96 well to 384-well plate samples to be analyzed in less than 7 mins, which greatly reduces the time required for the single sample based automated cell counter.
Shan, Feng July 2016 Investigation of cancer drug penetration in 2D and 3D tumor cell culture models University of Pittsburgh Cal33 HNSCC cells  
Bonasu, Surekha July 2016 Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo Image cytometer Poster Jurkat cells Monitoring by detecting a green caspase 3/7 signal but also perform an end point assay by counterstaining the cells with Hoechst and therby determined a percent nucleated cells that are apoptosis by imaging on the Nexcelom Celigo image cytometer.
Chan, Leo Li-Yang July 2016 A high-throughput image cytometry-based screening method for the cytotoxic effect of drug compounds on 3D tumor spheroid Cancer Research Cancer cells Five experiments were conducted demonstrating comparable results in 2D and 3D models using the image based high-throughput screening method for 3D tumor spheroids on 384- well ultra low attachment round bottom microplates using the Celigo image cytometer.
Shirihai, Orian July 2016 Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicity The Journal of Cell Biology B- cells Analysis parameters for images acquired by the Celigo were optimized to identify individual cells based on fluorescence. Cells were then fixed in 4% paraformaldehyde, stained with DAPI, and imaged using Celigo to count the total number of cells per well for normalization.
Chan, Leo Li-Ying June 2016 High-throughput 3D tumor spheroid screening method for cancer drug discovery using Celigo Image Cytometry Journal of Laboratory Automation U87MG Celigo was used to determine the seeding density for the tumor sphere formation of the cell line U87MG, and it was to measure the IC50 value, and the dose responses of 17-AGG, paclitaxel, TMZ and doxorubicin
Ellegaard, Anne-Marie June 2016 Repurposing Cationic amphiphilic antihistamines for cancer treatment EbioMedicine Non-small cell lung cancer (NSCLC) Celigo measured the cell death after 15 minutes of propidium iodide and Hoechst-33342 staining was employeed at 37 degrees celcius.
Mazor, Yariv June 2016 Enhancement of Immune Effector Functions by modulating IgG’s intrinsic affinity for target antigen Immune cells, T-cells Immune cells, T-cells Target cells were stained with Calcein AM dye, and then seeded in a 96 well plate at a density of 1×10^4 cells/well in RPMI in 1640 with GlutaMAX with 5% FBS. Calcein AM positive cells were quantified by Celigo.
Napoli, Marco June 2016 ΔNp63/DGCR8- Dependent MicroRNAs mediate therapeutic efficacy of HDAC inhibitors in Cancer Cancer cell Squamous cell carcinomas and lymphoma cells To acess the efficacy of the compounds in affecting the protein levels of ΔNp63 and DGCR8, evaluating cells by immunofluoresence and quantified them with Celigo.
Patterson, Lisa May 2016 In vitro assays for endothelial cell functions required for angiogenesis: Proliferation, motility, tubular differentiation, and matrix proteolysis Angiogenesis Protocols Umbilical vein endothelial cells, microvascular endthothelial cells Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Kildegaard, Helene Faustrup May 2016 Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment Biotechnology & Bioengineering CHO cells Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Tomokazu, Tanaka April 2016 Low-dose farnesyltransferase inhibitor suppresses HIF-1a and snail expression in Triple-negative breast cancer MDA-MB-231 cells in vitro Journal of Cellular Physiology MDA-MB-TNBC and MDA-MB-231 cells Tumorspheres were directly counted by the Celigo Cell Cytometer at a 12-day culture. Pictures of each well were taken to conform spheres and aggregates of cells.
Mattson, Emma April 2016 Understanding the role of RB-binding protein KDM5A in cancer cell proliferation Illinois Mathematics and Science Academy lung cancer cells Focused on clarifying the role of KDM5A in a non-small cell lung cancer line under different concentrations of erlotinib, a common chemotherapy drug, by examinging the cell cycle and cell proliferation using the Celigo.
Elisabeth, Corecelle-Termeau April 2016 Excess sphingomyelin disturbsATG9A trafficking and autophagosome closure Autophagy SMPD-1- deficient cells Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Amy, Wagers April 2016 Methods and compositions forrejuvenating skeletal muscle stem cells FPO Skeletal muscle stem cells Wells containing myogenic colonies were either conunted by brightfield microscopy or fixed with 4% PFA and the number of cells per well was counted on a Celigo automated microscope as Hoechst-stained nuclei at the indicated time points
Gayathri, Devi April 2016 Three-Dimensional culturesystems in cancer research: Focus on tumor spheroid model Pharmacology & Therapeutics Cancer stem cells Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity, and structural complexity that reflecdt in vivo tumors.
Bidisha, Bhattacharya April 2016 Fine-tuning of macrophageactivation using synthetic rocaglate derivates Scientific Reports J7-21 cells Celigo cytometer was used ot measure number of GFP-Ipr1 positive cells and GFP-Ipr1 fluorescence intensity per nucleus after counterstaining with nuclear stain DAPI.
Peitzsch, Claudia March 2016 An epigenetic reprogramming strategy to re-sensitize radioresistant prostate cancer cells Cancer Research Cancer stem cells After 14 days, cells were anaylzed and automatically scanned using the Celigo for 30 mins at 37 degrees celcius, washed with PBS at the end of the culture, then analyzed by the Celigo.
Stanford, Elizabeth March 2016 The role of the aryl hydrocarbonreceptor in the development of the cells with the molecular and functionalcharacteristics of cancer stem- like cells BMC Biology Breast cancer stem cells After cells were treated, harvested, dosed, and plated colonies were quantified with the Celigo after eight days.
Mohammad, Tariq January 2016 Eukaryotic translationinitiation factor 5A (elF5A) is essential for HIF-1a activiation in hypoxia Biochemical and Biophysical research communications Tumor spheroids Suppression of elF5A by siRNA oligomediated knockdown or treamtent with GC7, a deoxyhypusine synthase inhibitor, led to significant reduction of HIF-1a activity
Edwin, Chang November 2015 AshwaMAX and withaferin Ainhbits gliomas in cellular and murine orthotopic models Journal of Neuro-oncology GBM2, GBM39 Human parietal-cortical gliobastoma cells (GBM2, GBM39) were isolated from primary tumours while U87-MG was obtained commerically.
Linfeng, Li November 2015 High-throughput imaging:Focusing in on drug discovery in 3D Methods MCT cells 3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery
Henning, Gram Hensen December 2015 Versatile microscale screeningplatform for improving recombinant protein productivity in chinese hamsterovary cells Scientific Reports Ovarian cells The platform consists of four techniques compatible with 96-well microplates:lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation
Sivapriya, Ponnurangam December 2015 Quinomycin A targets notchsignaling pathway in pancreatic cancer stem cells Oncotarget CSC cells Quinomycin treatment resulted in significant inhibition of proliferation and colony formation in pancreatic cancer cell lines, but not in normal pancreatic epithelial cells
Annika, Baude December 2015 Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination Nucleic Acids research HeLa cervic carcinoma cells POGZ-depleted cells show a similar defect in DNA damage-induced RPA2 phosphorylation as HDGFRP2-depleted cells
Andrew, Campbell October 2015 Mutation ofataxia-telangiectasia mutated is associated with dysfunctional glutathionehomeostasis in cerebellar astroglia Glia Mouse cells Cerebellar atroglia isolated from Atm mutant mice show decreased expression of the cystine/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione-S-transferase.
Thilo, Riedl October 2015 High-throughput screening forinternalizing antibodies by homogeneous fluorescence imaging of apH-activated probe Journal of Biomolecular Screening A431, AU565, SKOV-3 cells The pH-activated CypHer5E label becomes fluorescent upon internalization into the acidic environment of endocytic organelles, whereas background fluorescence of noninternalized CypHer5E is minimal.
Narendran, Aru September 2015 Targeted inhibition of MEK1 bycobimetinib leads to differentiation and apoptosis in neuroblastoma cells BioMed central None  
Simeonov, Anton September 2015 High- throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models Expert Opinion Drug Discovery None  
Yoshida, Minoru September 2015 Identification of thedeterminants of 2-deoxyglucose sensitivity in cancer cells by shRNA libraryscreening ScienceDirect Cancer cells  
Kaji, Keisuke September 2015 Reprogramming roadblocks are system dependent ISSCR (iPSC’s)  
Anant, Shrikant August 2015 RNA binding protein RBM3increases B-catenin signaling to increase stem cell characteristics incolorectal cancer cells Wiley Online Library Cancer stem cells  
McGlennen, R July 2015 Comparison of Antimicrobial andwound healing agents on oral fibroblast viability and In-vivo bacterial load Omics Online Gingival fibroblast cells  
Dobbelstein, Matthias July 2015 MicroRNA-101 suppresses tumorcell proliferation by acting as an endogenous proteasome inhibitor viatargeting the proteasome assembly factor POMP ScienceDirect Tumor cells  
Dubrovska, Anna June 2015 Development of novelradiochemotherapy approaches targeting prostate tumor progenitor cells usingnanohybrids International Journal of Cancer Cancer stem cells  
Gahl, William June 2015 Automated, High-throughputderivation, characterization and differentiation of induced pluripotent stemcells Nature Methods (iPSC’s)  
Mermod, Nicolas June 2015 Epigenetic regulatory elements:Recent advances in understanding their mode of action and use for recombinantprotein production in mammalian cells Biotechnology Journal mammalian cells  
Lee, Dan May 2015 High- throughput direct cell counting- based natural killer cell mediated- cytotoxicity assay using Celigo Imaging Cytometry The Journal of Immunology NK cells  
Sun, Yi May 2015 A reliable parameter tostandardize the scoring of stem cell spheres PLOS one Mouse embryonic stem cells  
Jaattela, Marja May 2015 Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay Autophagy    
Chang, Stephen May 2015 Rapid and efficient generationof transgene-free iPSC from a small volume of cryopreserved blood Stem cell reviews and reports hESC online
Kildegaard, Helene April 2015 One-step generation of tripleknockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment Biotechnology Journal CHO Online
Leach, M.O March 2015 Acquired resistance to EGFRtyrosine kinase inhibitors alters the metabolism of human head and necksquamous carcinoma cells and xenograft tumors British Journal of Cancer CAL/PJ HNSCC online
Nabergoj, Dominik, Elsa December 2014 Evaluation of anti-inflammatory and proapoptotic activities of synthetic clathrodin analogues in human THP-1 monocytic Leukemia cells Thesis THP-1 cells online
Flores, Elsa November 2014 Non-disruptively count and quantify fluorescence iPS colonies during 2 degree reprogramming: 7 min per 6 well plate, dual- fluorescence whole well imaging cytometry Nature Mouse embryonic stem cells(G4) online
Kolev, Vihren November 2014 P13K/mTOR dual inhibitor VS-5584preferentially target cancer stem cells American Association for Cancer Research Cancer stem cells To detemine tumorsphere forming efficency, cells from the tissue culture were plated and enumerated using the Celigo.
Karlin, Mondal November 2014 The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor Cell Reports BT549 and MDA-MB231-LM2 breast cancer cells Celigo was used to perform cell proliferation assays.
Westbrook, Thomas November 2014 The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor Cell Reports None online
Miura, Hiromi October 2014 Development of spheroid based high-throughput screening of cell-cell adhesion inhibitors to reverse acquired multicellular resistance Cancer Research A549 online
Randhawa, Zeng October 2014 Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs Antiviral Research HEK293, COS7 Cell counts were evaluated by Celigo to ascertain cytotoxicity of compounds.
Glicksman, Marcie A. October 2014 Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs Antiviral Research HEK293, COS7 online
Landmann, Proia September 2014 UDP glucuraonosyltransferase 1Aexpression levels determine the response of colorectal cancer cells to theheat shock protein 90 inhibitor ganetespib Cell Death and Disease human colorectal cancer cell lines Cell confluence in drug treated and untreated wells was determined by Celigo with bright field.
Fu, Creighton September 2014 Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase Breast Cancer Research MCF-7L, BT483, and T47D (human breast cancer) Cell count was assessed on Celigo via methylene blue and bright field.
Schiff, R September 2014 Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase Breast Cancer Research MCF7L (Human Breast Cancer), BT483, T47D Online
Xu, Farach-Carson July 2014 Three-dimensional in vitro tumormodels for cancer research and drug evaluation Biotechnology Advances tissue engineered-3D tumor models Celigo measured spheroid diameter, permieter, and area which enabled assessments of motility, angiogenesis, and matrix invasion, as well as the actions of cell growth inhibitors.
Brix, Rafn July 2014 Screening and identification ofsmall molecule inhibitors of ErbB2-induced invasion Molecular Oncology MCF-7 (human breast cancer) and SK-OV3 (human ovarian) Cells were stained with Hoechst and PI and Celigo ascertained the total cell counts as well as number of dead cells.
Jayanthan, Ruan July 2014 Aurora kinases as druggabletargets in pediatric leukemia: heterogeneity in target modulation activitiesand cytotoxicity by diverse novel therapeutic agents PLOS One pediatric and infant leukemia cell lines Cell lines were stained with Alamar blue to assess cells survival via metabolic activity.
Scheerlinck, Van Steendam June 2014 Detailed method description fornoninvasive monitoring of differentiation status of human embryonic stemcells Analytical Biochemistry human embryonic stem cells (hESCs) Celigo evaluated colony confluence and measurement of stress level via Oct 4 expression.
Pederson, Ronda May 2014 Accelerating genome editing inCHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool Biotechnology and Bioengineering CHO cells Two-channel bright field and GFP images were captured by Celigo to count cells and determine transfection efficiency.
Lund, Kildegaard May 2014 A versatile system for USERcloning-based assembly of expression vectors for mammalian cell engineering PLOS One CHO-S (Chinese hamster ovary clonal isolate) Cells were plated in 6-well plates and transfected with GFP-tagged vectors before the addition of Hoechst stain. Two-channel (Hoechst and GFP) images were collected on the Celigo to determine cell count and transfection efficiency.
Guo, Loh May 2014 Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening Molecular Pharmaceutics MCF-7 (human breast cancer) Bright field images of the spheroids in 96-well plates were generated over a time course by Celigo. Growth kinetics of spheroid diameter, perimeter, and area were captured.
Jaiswal, Lauritzen May 2014 S100A11 is required forefficient plasma membrane repair and survival of invasive cancer cells Nature Communications MCF-7 (human breast cancer) Cell death was determined by Celigo using propidium idodide (PI) 96 hours after control or siRNA delivery.
Yang, Chung May 2014 Systems analysis of the prostatetumor suppressor NKX3.1 supports roles in DNA repiar and luminal celldifferentiation F1000 Research LNCaP (human prostate cancer) Cells were dual stained with Heochst and Alexa Fluor 594 and imaged by Celigo to ascertain cell proliferation.
Guo, Loh May 2014 Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening Molecular Pharmaceutics multicellular tumor spheroids Celigo captured bright field images over time to determine diameter, perimeter, and area of the spheroids with or without drug treatment.
Kalineo, Thein April 2014 Acetaminophen and NAPQI aretoxic to auditory cells via oxidative and endoplasmic reticulumstress-dependent pathways Hearing Research HEI-OC1 cells (murine-derived auditory cells) Celigo performed direct cell counts after treatment with control or drug over a time course.
Spike, Kelber April 2014 CRIPTO/GRP78 signaling maintainsfetal and adult mammary stem cells ex vivo Stem Cell Reports MCF10A and fetal mammary stem cells Celigo measured DNA content via Hoechst and cell proliferation through bright field images.
Vissapragada, Contreras March 2014 Bidirectional crosstalk betweenperiventricular endothelial cells and neural progenitor cells promotes theformation of a neurovascular unit Brain Research Periventricular region endothelial cells (PVEC) and neural progenitor cells (NPC) Cells were imaged by Celigo in 96-well Matrigel coated plates to ascertain the length and pixel intensity of 3D cords created by GFP-labeled ECs.
Holembowski, Kramer March 2014 TAp73 is essential for germ celladhesion and maturation in testis Journal of Cell Biology Germ-Stertoli cell co-cultures Celigo captured two-channel images (bright field and GFP) to quantify cells after lentiviral infection of adhesion genes.
Emhemmed, Azouaou March 2014 Selective anticancer effects ofa synthetic flavagline on human Oct4-expressing cancer stem-like cells via ap38 MAPK-dependent caspase-3-dependent pathway Biochemical Pharmacology NT2/D1 (human embryonal carcinoma) Celigo determined the rate of apoptosis via Annexin V/PI staining after flavagline derivative FL3 application.
Trapnell, Cacchiarelli March 2014 The dynamics and regulators ofcell fate decisions are revealed by pseudotemporal ordering of single cells Nature Biotechnology human skeletal musucle myoblasts Celigo performed whole well imaging with Hoechst and markers to determine the number of cells or nuclei, fraction of nueclei in MYH2- or CD13-positive cells, and total MYH2-positive area.
Shih, Chung-Hsuan February 2014 Astroglial-Derived PeriostinPromotes Axonal Regeneration after Spinal Cord Injury The Journal of Neuroscience Rat Neurites Neurite length was measured using Celigo cytometer to quantify the confluence of TujI-stained neurites, providing a quantitative measurement of neurite outgrowth.
Fu, Morrison February 2014 Therapeutic potential of thedual EGFR/HER2 inhibitor AZD8931 in circumventing endocrine resistance Breast Cancer Research Treatment Tamoxifen resistant (TamRes) MCF-7 and T47D cells (both breast cancer) Cells counts and rate of apoptosis via Annexin V-FITC staining was determined by Celigo.
Schulz, Ramona January 2014 HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer Cell Death and Disease MDA-MB-453, and SK-BR-3 (Human Breast Cancer) Cells were seeded and cultured for 1 day, then treated with CP724.714 (CP) for 24hr or left untreated and followed up to day 5. Cell confluence measured daily by Celigo. For cell survival, equal numbers of treated or untreated cells were plated into 12 well plates. With the Celigo cytometer, cell confluence was measured over indicated time periods w/Celigo
Sproul, Andrew January 2014 Characterization and MolecularProfiling of PSEN1 Familial Alzheimers Disease iPSC Derived NeuralProgenitors PLOS one Fibroblast 11 and 11C, reprogramed to iPSCs Immunostaining was performed. Hoechst was used to visualize DNA. The following antibodies were used: OCT4, SSea4, Nanog, Tra-160, Ki67, MAP2, Nestin, NeuNm TujI, NLRP2, NDP. Quantification of immunostaining was done on the Celigo 200-BFFL
Giovannini, Marco January 2014 mTORC1 Inhibition Delays Growthof Neurofibromatosis Type 2 Schwannoma Neuro-Oncology Human Primary Schwann cells Cells were fized in 4% paraformaldehyde and stained with anti-S100 protein,, followed by AI549-conjugated goat anti-rabbit secondary antibody and counterstained with Hoechst 33258. Cell surface areas were determined using the automated Celigo Cytometer.
Postupalenko, Viktoriia January 2014 Intracellular Delivery ofFunctionally Active Proteins Using Self-AssemblingPyridulthiourea-polyehtlenimine Journal of Controlled Release U87 (Human Glioma), CaSki (Human small bowel mesentery), HeLa (Human Cervical Cancer) Cells were seeded into 96-well plates at 10 4 cells/well the day before . Flow cytometry analysis was performed with a Celigo Cytometer after a 24 h incubation period on a suspension of freshly trypsinized cells
Postupalenko, Sibler January 2014 Intracellular delivery offunctionally active proteins using self-assemblingpyridylthiourea-polyethylenimine Journal of Controlled Release U87 (human primary glioblastoma) Cells were plated in 96-well plates and treated with GFP polyplexes. Celigo was used to determine intracellular eGFP delivery.
Schulz, R. January 2014 HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer Cell Death and Disease    
Ferree, Andrew 2014 Supplemental Material-characterization and molecular profiling of PSEN1 Landes Bioscience    
Venkatanarayan, Raulji 2014 IAPP-driven metabolicreprogramming induces regression of p53-deficient tumours in vivo Nature H1299 (human lung adenocarcinoma) Rates of apoptosis (via Annexin V/PI staining) and ROS generation (via CellRox Deep Red) were determined by Celigo.
Chen, Hsing-Yu March 2013 Inhibition of red/Fyn/c-CBLPathway Function by Cdc42 Controls Tumour Initiation Capacity and TamoxifenSensitivity in Basal-like Breast Cancer Cells EMBO Molecular Medicine MDA-MB-231, MDA-MB-468, Hs578T, HCC1569, MCF-7m HCC38, HCC70, HCC1954, MCF-10A (Human Breast Cancer) Cells were incubated with Calcein AM and PI for 15 mins, after which live and dead cells were counted utilizing the Celigo Cytometer. Levels of intracellular stress of oxidation were determined by CMH2DCFDA staining to the manufacturers instructions. Briefly cells were incubated with CM-H2DCFDA in the phenol red free medium in teh dark for 30 min, after which cells were washed once and fluorescent intensity of cells was determined using Celigo.
Proschel, Christoph December 2013 Delayed Transplantation ofPrecursor Cell-Derived Astrocytes Provides Multiple Benefits in a Rat Modelof Parkinsons EMBO Molecular Medicine Cortical and dopaminergi mid brain neurons isolated from E18.5 Sprague-Dawley rats. Cell survival was determined by co-labelling cultures with TujI and anti-tyrosine hydroxulase antibody. TujI+ or TujI+/TH+ neurons were counted using a Celigo Cytometer.
Neradugomma, Naveen December 2013 Prolactin Signalling EnhancesColon Cancer Stemness by Modulating Notch Singaling in a Jak2-STAT3/ERKManner Carcinogenesis Colon cancer cell LinesHT29, HCT116, SW480, SW620, DLD1, and normal intenstinal epithelial fetal human colon (FHC) cells Colosphere formation was assessed after 4-6 days, the number and size of colonospheres was determined using Celigo
Xu, Cong November 2013 A Zebrafish Embryo CultureSystem Define Factors that Promote Vertebrate Myogenesis Across Species Cell Zebrafish cells Zebra fish cells were iamged after 2 days using a Celigo cytometer for GFP, mCherry and BR signals.
Neradugomma, N. November 2013 Prolactin induces Notchsignaling in a Jak2-STAT and Jak2-ERK pathway in colon cancer stem andprogenitor cells Carcinogenesis    
Zangi, Lior September 2013 Modified mRNA Directs the Fateof Heart Progenitor Cells and Induces Vascular Regeneration After MyocardialInfarction Nature Biotechnology Skeletal muscle myotubes differentiated from primary mouse satellite cells Cells were harvested and equal numbers were plated in each well of a 96-well plate in growth media. Cells were transfected with modified RNA after 3 d in differentiation media. Myotubes were transfected w/0, 0.3, 1 or 3 µg of GFP-modified RNA. Cells were fixed 16 h after transfection. Transfected myotubes were stained for skeletal muscle myosin heavy chain, 10 µg/ml Hoechst and rabbit anti-GFP Alexa-488. Pictures were taken from the whole well using the Celigo cytometer under blue, red and green channels. Cell viability was measured by quantifying the total number of nuclei in the transfected wells 16 h after transfection and normalizing them to the total number of nuclei in the non-transfected wells.
Chen, Hsing-Yu September 2013 MEK1/2 Inhibition SuppressesTomixfen Toxicity on CNS Glial Progenitor Cells The Journal of Neuroscience O-2A/OPC (Astrocyte progentior/oligodendrocyte precursor cells), GRP (Glial-restricted precursor cells), astrocytes, neuroepithelial stem cells and oligodendrocytes Cell number and death of O-2A/OPCs were analyzed by Calcein AM and PI Staining in live culture using a Celigo Cytometer.
Ferree, Andrew September 2013 MitoTimer Probe Reveals theImpact of Autophagy, Fusion and Motility on Subcellular Distribution of Youngand Old Mitochondrial Protein and on Relative Mitochondrial Protein Age Autophagy MEF (Human Breast Cancer), COS (Monkey Kidney Tissue) To quanity red and green fluorescence intensity per cell in MEF and COS cells, cells were imaged using Celigo. Analysis parameters for images acquired by Celigo were optimized to identify individual MEF and COS cell based on fluorescence and the avg. green and red fluorescnce intensity for each cell was determined at the different time points.
Wang, Ying August 2013 Scalable Expansion of HumanInduced Pluripotent Stem Cells in the Defined Xeno-fress E8 Medium UnderAdherent and Suspension Culture Conditions Journal of Stem Cell Research BC1 (human primary effusion lymphoma ), TNC1 (Rat type 1 astrocytes) The Total # of cell aggregates in a 1ml sample was analyzed by using the embryoid body analysis module in Celigo Imaging Cytometer. The avg. equivalent diameter of aggregates and the szie of each aggregate in the sample were also measured.
He, Xianbao August 2013 The sst1 Resistance LocusRegulates Evasion of Type 1 Interferon Signalling by Chlamydia pneumoniae asa Disease Tolerance Mechanism PLOS one: Pathogens Bone Marrow Derived Macrophages T-cell survival Assay: Cells were plated and treated with infected C. pneumoniae and at designated time points were stained with Hoechst and PI. Total cell number and dead cell number were counted using Celigo Cytometer
Li, Jianqing June 2013 A Short Hairpin RNA Screen ofInterferon-Stimulated Genes Identifies a Novel Negative Regulator of theCellular Antiviral Response Mbio. Vero T144 (Kidney Epithelial Cells), NIH 3T3 (Human Fibroblasts), Hek293T (Human Embryonic Kideny cells), HeLa (Human Cervical Cancer) HeLa cells in 96-well plates were transfected with individual ISGs tagged with 3X Flag. After 24h, cells were infected w/WNV for another 24h and then fixed, permeabilized, and costained for WNV envelope protein (MAbE18)and the nucleus using DAPI. Images were captured and processed using a Celigo cytometer (Cyntellect). Infected and uninfected populations were gated separately, and infectivity was measured as the % of infected cells from the total cell counts.
Huang, Haiqing June 2013 iPSC-Derived B-Cells ModelDiabetes Due to Glucokinase Deficiency Journal of Clincial Investigation Patient Cell derived IPS to B-cell Model Quantification of positively stained cells was performed using the celigo cytometer system.
Bian, Shu April 2013 P2X7 Integrates PI2k/AKT andAMPK-PRAS40-mTOR Signaling Pathways to Mediate Tumor Cell Death PLOS one MC138 (Colon Cancer Cell Line), B16/F10 (Melanoma cell line) Cells were seeded into Corning 3603 Black 96-well plates and grown for 24 hr before exposed to ATP for a short period of time. 16–24 hr later, cell growth was evaluated using the Celigo. BR images of live cells were captured using the Celigo Cell Counting application
Escribano-Diaz, Cristina March 2013 A Cell Cycle Dependent RegulatoryCircuit Composed of 53BP1-RIF1 and BRCA1-CtlP Controls DNA Repair PathwayChoice Molecular Cell U2OS (human Osteosarcoma) U2OS cells were transfected with siRNA in 96-well plates. At 48 hr post-transfection cells were treated w/neocarzinostatin for 15 min. After a 3 hr recovery, cells were extracted with 0.2% Triton X-100 in PBS for 10 min on ice, fixed with 4% paraformaldehyde (PFA), and processed for RPA32 immofluorescence. Nuclei were counterstained with DAPI. Cells were imaged using the Celigo laser scanning plate cytometer (Brooks Automation) and analyzed with the accompanying image analysis software.
Vinci, Maria January 2013 Tumor Spheroid Based Migration Assays for Evaluation of Therapeutic Agents Methods in Molecular Biology    
Lesovaya, Ekaterina January 2013 Combination of a SelectiveActivator of the Glucocorticoid Receptor Compound A with a Protease Inhibitoras a Novel Strategy for Chemotherapy of Hematologic Malignancies Cell Cycle CEM (T- cell acute lymphoblastoma leukemia), K562 ( chronic myeloblastic leukemia), NCEB (mantle cell lymphoma) and multiple MM.1R (glucocorticoid resistant) and MM1S (glucocorticoid sensitive) The proliferation was measured by direct cell counting using Celigo Cytometer. Cells were plated at 10^4/well onto 24-well plates and cultured in complete medium in the presence of CdpA, Dex, Bortexomib or vehicle.
Signh, Anjali January 2013 Profiliing Pathway SpecificNovel Therapeutics in Preclinical Assessment for CNS Atypical TeratoidRhabdoid Tumors Molecular Oncology BT12 and BT16 (CNS ATRT), KCCF1 (cerebral spinal fluid), Hs68 (Primary skin fibroblast), T98G [EGFR over-expressing glioblastoma multiform (GBM)] ATRT cells were trypsinized and placed in 96-well plates @ a concentration of 5×10^3 cells per well. Increasing concentrations of study agents were added to these wells to a final volu,e of 200ul per well. After 4 days, cell survival was quantified by Celigo Cytometer
Al-kasspooles, Mazin January 2013 Preclincal Antitumor Activity ofa Nanoparticulate SN38 Journal of Investigative New Drugs HT29, HCT116 (Human Colorectal Carcinoma) and H-meso-1 H226, H2052, MSTO-211H (Human Mesothelioma) Cells were plated in 96-well black clear bottom plates. After overnight incubation, PI was added and live and dead cell numbers at time 0 were determined using the Celiigo cytometer. Drug treatments were then added to cells . Live and dead cell numbers were analyzed again 24, 48, and 72 after drug addition.
Singh, Lun January 2013 Profiling pathway-specific noveltherapeutics in preclinical assessment for central nervous system atypicalteratoid rhabdoid tumors (CNS ATRT): Favorable activity of targeting EGFR-ErbB2 signaling with lapatinib Molecular Oncology atypical teratoid rhabdoid tumor (ATRT) cells Cell survival in 96-well plates was determined by Celigo in bright field after 4 days treatment with drug or control.
Stecklein, August 2012 SI Methods PNAS    
Golipour, Azadeh December 2012 A Late Transition in SomaticCell Reprogramming Requires Regulators Distinct From the Pluripotency Network Cell: Stem Cell MEFs Secondary MEFs as indicated in the schematics were stained for AO and DAPI and then scanned with a Celigo hgih-content microscope to quantigy AP-positive areas. Dox withdrawals was quantified for 4 SC and 4 SI clones using the Celigo system. In both screens, 3 days after transfection, cells were fixed and stained for Dppa24, counterstained with DAPI and then subjected to automated analysis using the Celigo system for quantifying colony formation
Smith, Karen September 2012 Human Family with SequenceSimilarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3Deacetylase Complex Molecular Cell Proteomic A549, HepG2 (Human Liver Cancer) A549 or HepG2 cells were transfected with control siRNAs or siRNAs against FAM60A. 3 days after transfection, cells were incubated for 5 hrs (A549) or 20hrs (HepG2) with BrdU. Cells were costained with DAPI. % incorporation of BrdU in each well was measured by fluorescence analysis using a Celigo Ceytometer
Stecklein, Shane August 2012 BRCA1 amd HSP90 Cooperate inHomologous and Non-Homologous DNA Double Strand Break Repair and G2/MCheckpoint Activation PNAS HCC1937 (Human Breast Cancer) DNA Synthesis, apoptosis and cell cycle distribution experiments in HCC1937 cells were performed on an LSRII flow cytometer or a Celigo adherent cell cytometer.
Nabzdyk, Christoph August 2012 Differential Susceptibility ofHuman Primary Aortic and Coronary Artery Vascular Cells to RNA Interference Journal of Biochemical and Biophysical Research Communications Endothelial (EC) and vascular smooth muscle (SMC) cells Cells were seeded at a density of 5000 cells/well of a 96-well plate. 24 hrs later cells were transfected with either non-targeting siRNA or non-targeting fluorescent red labelled siRNA using no transfection reagent., HiPerfect or Lipfectamine RNAiMax. Hoechst nuclei stain was used to label cells for counting. For data analysis an adherent cell cytometer Celigo was used.
McKevitt, I. April 2012 SARS ORAL 2012 British Journal Of Surgery Society Ltd.    
Borrego-Diaz, Emma April 2012 Overactivation of Ras SignalingPathway in CD133+ MPNST Cells Journal of Neurooncology MCF7L (Human Breast Cancer) Functional analysis of cell growth and apoptosis was performed following knockdown of miRNAs using insitu cell cytometry (Celigo)
Healy, N.A. April 2012 The Role of miRNAs in TamoxifenResistance in Breast Cancer Cellular and Molecular Life Sciences Human MPNST primary cells and mouse MPNSTs, S805, S462, T530, T532, SW10 (mouse Schwann cells) and HSCs The spheres were counted after 40hr, 7 days and 10 days of incubation using both Celigo and a light microscope.
Hsieh, Jeng-Long March 2012 Acquisition of an EnhancedAggressive Phenotypein Human Lung Cancer Cells Slected by Suboptimal Doses ofCisplatin Following Cell Deattachment and Reattachment Journal of Cancer Letters A549, H1299, H1299-RI~H1299-R5 (Human Lung Cancer Cell Lines) To analyze cell proliferation 1000 cells were seeded in 96-well plates in the complete medium at 37C on day 0. The number of cells was counted daily from day 1 to day 4 using a Celigo cytometer according to themanufacturers instructions. The proliferation rate is expressed as a ratio of the numberof cells counted on days 2-4 by the number counted on day 1
Vinci, Maria March 2012 Advances in Establishment andAnalysis of 3D Tumor Spheroid-based Functional Assays for Target Validationand Drug Evaluation BMC Biology U-87 MG glioblastoma, SF188 glioblastoma, MDA-MB-231 (Human Breast Carcinoma),…37 other cancer cells lines. For rapid, routine imaging and analysis of tumor spheroids, we utilized a Celigo cytometer, which is a bench top in situ celluar analysis system providing high quality, ful or partial images of wells using BR or FL illumination.
Huang, Han-Hung February 2012 A Replacement for IsletEquivalents with Improved Reliability and Validity Acta Diabetologica Murine Pancreatic Islet cells Isolated islet cells were iamged and counted with Celigo Cytometer
Ponnurangam, Sivapriya February 2012 Honokiol in Combination withRadiation Targets Notch Signalling to Inhibit Colon Cancer Stem Cells Molecular Cancer Therapeutics HCT-116 (Human Colon Carcinoma), SW480 (Human Adenocarcinoma of the colon) Colonosphere assay: The number and size of colonosphere were determined using Celigo
Schulz, Ramona January 2012 Inhibiting the HSP90 ChaparoneDestabilizes Macrophage Migration Inhibitory Factor and Thereby InhibitsBreast Tumor Progression Journal of Experimental Medicine U2SO (Osteosarcoma), SW480 (Human adenocarcinoma of the colon) Cell Confluence and cell numbers were evaluted over time by the Celigo Cytometer. Cell numbers (U2OS) or cell confluence (SW480) were measured using the Celigo cytometer using 49 squares per well
Gall, Jonathan January 2012 Role of Mitofusin 2 in the RenalStress Response PLOS one Primary Murine Kidney Cells Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS and stained with PBS containing Hoechst for 30 min before being imaged and counted by Celigo cytometer.
Li, Dan January 2012 The Role of CYP3A4 mRNATranscript with Shortened 3′-Untranslated Region in HepatocyteDifferentiation, Liver Development and Repsonse to Drug Induction Molecular Pharmacology HepaRG Cells (Human Hepatic Cells) The expression of RFP was analyzed in situ on an adherent cell cytometer to obtain cells images in both BR and red fuorescence, The ratios of cell densities from BR to red fluorescence in each entire well were analyzed by Celigo software.
Yoshida, Shunsuke November 2011 Thrombospondin-2 Gene Silencingin Human Aortic Smooth Muscle Cells Improves Cell Attachment Journal of the American College of Surgery HAoSMC (Human Aortic Smooth Muscle Cell) Scratch assay (wound healing attchment assay): cell positions were determined using BR and Confluence modes of Celigo, and cell counting of cells stained with Hoechst was performed
Majumdar, Ishita November 2011 Synthetic Cyclohexenyl ChalconeNatural Products Possess Cytotoxic ActivitiesAgainst Prostate Cancer Cellsand Inhibit Cysteine Cathepsins in Vitro Journal of Biochemical and Biophysical Research Communications DU-145 and PC3 (Human Colon Cancer) Cells Apoptotic cells stained with Hoechst were quantitatively analyzed with the Celigo cyotmeter
Wang, Yen-Chao November 2011 Different Mechanisms forResistance to Trastuzumab Versus Lapatinib in HER2- Positive Breast Cancers,Role of Estrogen Receptor and HER2 Reactivation Breast Cancer Research BY474, UACC-812, AU-565, HCC-1569, HCC-1954, HCC-202, MDA-MB-361, MDA-MB-453, ZR75-30, SKBR-3, MCF7-HER2 (All Human Breast Cancer Cells were incubated with Annexin V-FITC and DAPI for 30 min and analyzed by the Celigo Cytometer for Apoptosis. Cells were stained with EdU and the proliferation rate was analyzed by the Celigo cytometer
Hoeksema, Kimberly September 2011 Systematic in-Vitro Evaluationof the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Setfor the Identification of a Candidate Drug Repertoire for MLL-RearrangedLeukemia OncoTargets and Therapy KOPN8 (B-Cell Precursor Leukemia), SEM (Human Acute Lymphoblastic Leukemia), B1 (Human B-cells), MOLT-3 (Human T-Lymphoblast), TIB-202 (acute monocytic leukemia) and Patient Bone Marrow Stromal (BMS) cells Bright Field cell counting, viable cell numbers count and counting of primary leikemia samples.
Las, Guy August 2011 Fatty Acids Suppress AutophagicTurnover in B-Cells Journal of Biological Chemistry INS1832/13 (Insulin-Producing ß-Cell Line) Images of cells stained with Hoescht and PI, in paper.
Wlodkowic, Donald May 2011 Wormomtry-on-a-Chip: InnovativeTechnologies for in Situ Analysis of Small Multicellular Organisms Cytometry Part A. None Mentioned as an example of an image cytometer
Lesovaya, E.A. May 2011 Antitumor Effect of Non-stroidGlucocorticoid Receptor Ligand CpdA on Leukemia Cell Lines CEM and K562 Biochemistry (Moscow) K562 (human myelogenous leukemia) cells, CEM (human lymphoblastic leukemia), transfected to create CEM-NF-_B-GFP-Luc, K562-NF-_B-
GFP-Luc, CEM-AP1-GFP-Luc, and K562-AP1-GFP-Luc
Direct Count of suspension cells and a good cell count curve as an in paper figure
Nabzdyk, Christoph April 2011 High-Throughput RNAi AssayOptimization Using Adherent Cell Cytometry Journal of Translational Medicine Human Aortic Smooth Muscle Cells Cells were stained with Cell Tracker Green (Invitrogen) and with Hoechst nuclei stain. Plates were read using the adherent cell cytometer equipped with a brightfield and three fluorescent channels: a blue filter for the Hoechst, red filter for the siGLO Red, and green for the Cell Tracker Green cytoplasmic dye.
Tingen, Candace March 2011 A Macrophage and ThecaCell-Enriched Stromal Cell Population Influences Growth and Survival ofImmature Murine Follicles in Vitro Society for Reproduction and Fertility Live, adhered ovarian stroma cells (Mouse) Imaging and cell counts taken from cells stained with Hoechst and FITC
Feng, Lili March 2011 Vascular CD39/ENTPD1 DirectlyPromotes Tumor Cell Growth by Scavenging Extracellular AdenosineTriphosphate Neoplasia U251 (Human Neuronal Glioblastoma), MDA-MB-231 (Human Breast Adenocarcinoma), A431 (Human Epidermal Carcinoma) and 5637 (Human Bladder Carcinoma) Cell confluence was determined regularly for 16 days using the Celigo Cell Cytometer
Bug, M March 2011 Anthracyclines induce theAcculumation of Mutant p53 Through E2F1-Dependant and Independent Mechanisms Oncogene Luciferase-expressing B16/F10, C57BL/6 (mouse melanoma) and Syngeneic C57BL/6murineMCA38 (colon cancer) Cell growth of cells exposed to ATP was evaluated with images and cell counting using the Celigo counter. Images present in paper
Kaestner, Phillip February 2011 Therapeutic Targeting of theMitotic Spindle Checkpoint Through Nanoparticle-Mediated siRNA DeliveryInhibits Tumor Growth in Vivo Journal of Cancer Letters HCT116 Cells (Human Colon Carcinoma) Read cell numbers at 24hr increments
Wlodkowic, Donald July 2010 Cytometry in Cell NecrobiologyRevisited. Recent Advances and New Vistas Cytometry Part A. None Only mentioned as a comparable cytometric thenologu
Sirmaci, Asli May 2010 A Truncating Mutation inSERPINB6 is Associated with Autosomal Recessive Nonsyndromic SensorineuralHearing Loss The American Jounal of Human Genetics HeLa cells, (Human Cervical Cancer) Transfected with SERPINB6-WT-GFP and SERPINB6-MUT-GFP 3-Channel, 8-bit stitched images were generated covering whole wells to identify the surface area & # of cells along with fluorescent intensities.
Hart, C.P. May 2010 Product Focus: High-ContentScreening and Imaging: Instrumentation, Analysis and Applications Journal of Biomolecular Screening None Product focus, describeds product capabilities