Nexcelom Bioscience

Cellometer and Celigo Image Cytometers View all Products →

Nexcelom’s image cytometers give researchers increased control of their samples and the data generated from their cell-based assays. The Cellometer Vision CBA system requires 20 µL of sample in suspension and is a simple, quick solution for assays such as cell cycle, apoptosis, GFP transfection and surface marker analysis. The Celigo offers a plate-based high-throughput solution for adherent and suspension cells, eliminating the need to trypsinize cells and is ideal for a broad range of applications including 3D multicellular tumor spheroids, proliferation, cell line development, colony counting, wound healing, and more.

Cellometer Vision image cytometer

Cellometer Vision CBA

Advanced imaging with 2 interchangeable fluorescent optics modules for simple, single sample 20µl cell-based assays with flow-like data output.

Cellometer Auto T4 bright field automatic cell counter

Celigo Image Cytometer

A micro-well, plate-based multi-channel bright field and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells.

Cellometer and Celigo Image Cytometers at a Glance

Cell / Sample Type
Cell Line
X
X X X
Cultured Primary Cells
X
X X X
Algae
 
X
     
Platelets
X
     
Low Concentration Cell Lines
X
 
X X X
Yeast (Clean Sample)
X
     
Yeast (Messy Sample)
X
     
Primary cells (Messy Sample*)
  X X
PBMCs, Splenocytes, Stem Cells
X
  X X
Hepatocytes
X
  X X
Adipocytes***
X
X X X
Cell-Based Assay **
X
X
X X X
Apoptosis (Annexin V-FITC/PI)
X
X
  X X
Apoptosis (Caspase Activity)
X
X
  X X
Autophagy (CytoID-green)
X
X
     
Cell Proliferation (CFSE)
X
X
  X X
Cell Cycle (PI)
X
X
  X X
GFP Transfection
X
X
  X X
RFP Transfection
X
X
  X X
Mitochondrial Potential (JC-1)
X
X
  X X
Multi-drug Resistance (ABC Transporter)
X
X
  X X
Surface Marker Analysis
X
X
  X X
Vitality (Calcein-AM/PI)
X
X
  X X
Image Cytometry**
X
X
  X X
Imaging Capabilities
         
Fluorescence Intensity Measurements
X
X
  X X
Detection of Weak Fluorescence
X
X
  X X
Configuration
         
Objective Magnification
5X
10X
     
Bright field
X
X
X X X
Contains Single Fluorescence Optics Module
     
Contains Dual Fluorescence Optics Module
X
X
     
3 Fluorescent Channels
  X  
4 Fluorescent Channels
    X
Desktop Work Station Included
 
X X X
Optional Satellite Work Station Available
X X X
User-changeable optics module(s)
X
X
     
Software Features
         
Software for analysis of clumpy and irregular-shaped cells
X
X
X X X
Mean cell diameter and cell size distribution
X
X
X X X
Sample adjustment calculator to determine sample volume for desired cell number / concentration
X
X
     
Cell type wizard for creating new cell type parameters
X
X
     
IQ / OQ Option
X
X
     
GLP/GMP Audit Control Option
X
X
     
Specialized algorithms for analysis of adipocytes***
X
     
Specialized algorithms for analysis of hepatocytes
X
     
FCS Express Software for flow-like data reporting for cell-based assays
X
X
  X X
FCS Express Software for Image Cytometry
X
X
  X X
Performance Criteria
         
Analysis time
5-15 sec.
5-15 sec.
     
Cell size range
5-300µm
0.5-15µm
     
Sample volume
20 µl
20 µl
     
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter