//Cellometer and Celigo Image Cytometers
Cellometer and Celigo Image Cytometers2018-10-24T16:18:40+00:00

Cellometer and Celigo Image Cytometers

Nexcelom’s image cytometers give researchers increased control of their samples and the data generated from their cell-based assays. The Cellometer Vision CBA system requires 20 µL of sample in suspension and is a simple, quick solution for assays such as cell cycle, apoptosis, GFP transfection and surface marker analysis. The Celigo offers a plate-based high-throughput solution for adherent and suspension cells, eliminating the need to trypsinize cells and is ideal for a broad range of applications including 3D multicellular tumor spheroids, proliferation, cell line development, colony counting, wound healing, and more.ons.

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“Our lab has found the use of this instrument [Cellometer Vision CBA] very beneficial for data analysis. The device shortens the time of our protocol by at least 15 minutes which largely affects the lab’s overall efficiency with processing samples.”

University of Alabama at Birmingham

“The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user-friendly and the support staff is outstanding!”

Mayo Clinic

“So much faster than traditional cell counting!! I mostly do FBS tests & this requires counting many, many wells!!! I love that now I can do an hour and a half of painstaking cell counting in about 10 minutes!!! AWESOME!”

University of Virginia

“The Celigo Imaging Cytometer is efficient and reliable and it has helped both expedite the research process as well as give me peace of mind of its reliability!”

“We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results!”

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Cellometer Spectrum image cytometer

Advanced imaging with 2 interchangeable fluorescent optics modules for simple, single sample 20µl cell-based assays with flow-like data output.

Cellometer Vision CBA image cytometer

A micro-well, plate-based multi-channel bright field and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells.

Celigo imaging cytometer

Cellometer and Celigo Image Cytometers at a Glance

Cell / Sample Type

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Cell Line X
X
X X X
Cultured Primary Cells X
X
X X X
Algae X
 
X
Platelets X
X
Low Concentration Cell Lines X
X
 
X X X
Yeast (Clean Sample)
X
Yeast (Messy Sample)
X
Primary cells (Messy Sample*) X
X X
PBMCs, Splenocytes, Stem Cells X
X
X X
Hepatocytes X
X
X X
Adipocytes*** X
X
X X X
Cell-Based Assay ** X
X
X
X X X
Apoptosis (Annexin V-FITC/PI) X
X
X
X X
Apoptosis (Caspase Activity) X
X
X
X X
Autophagy (CytoID-green) X
X
X
Cell Proliferation (CFSE) X
X
X
X X
Cell Cycle (PI) X
X
X
X X
GFP Transfection X
X
X
X X
RFP Transfection X
X
X
X X
Mitochondrial Potential (JC-1)
X
X
X X
Multi-drug Resistance (ABC Transporter) X
X
X
X X
Surface Marker Analysis X
X
X
X X
Vitality (Calcein-AM/PI) X
X
X
X X
Image Cytometry** X
X
X
X X

Imaging Capabilities

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Fluorescence Intensity Measurements X
X
X
X X
Detection of Weak Fluorescence X
X
X
X X

Configuration

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Objective Magnification 5X
5X
10X
Bright field X
X
X
X X X
Contains Single Fluorescence Optics Module
Contains Dual Fluorescence Optics Module X
X
X
3 Fluorescent Channels
X
4 Fluorescent Channels
X
Desktop Work Station Included
X X X
Optional Satellite Work Station Available
X X X
User-changeable optics module(s)
X
X

Software Features

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Software for analysis of clumpy and irregular-shaped cells X
X
X
X X X
Mean cell diameter and cell size distribution X
X
X
X X X
Sample adjustment calculator to determine sample volume for desired cell number / concentration X
X
X
Cell type wizard for creating new cell type parameters X
X
X
IQ / OQ Option X
X
X
GLP/GMP Audit Control Option X
X
X
Specialized algorithms for analysis of adipocytes*** X
X
Specialized algorithms for analysis of hepatocytes X
X
FCS Express Software for flow-like data reporting for cell-based assays X
X
X
X X
FCS Express Software for Image Cytometry X
X
X
X X

Performance Criteria

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Analysis time 5-15 sec.
5-15 sec.
5-15 sec.
Cell size range 5-300µm
5-300µm
0.5-15µm
Sample volume 20 µl
20 µl
20 µl
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell-Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter