//Cellometer and Celigo Image Cytometers
Cellometer and Celigo Image Cytometers2018-09-10T19:00:10+00:00

Cellometer and Celigo Image Cytometers

Nexcelom’s image cytometers give researchers increased control of their samples and the data generated from their cell-based assays. The Cellometer Vision CBA system requires 20 µL of sample in suspension and is a simple, quick solution for assays such as cell cycle, apoptosis, GFP transfection and surface marker analysis. The Celigo offers a plate-based high-throughput solution for adherent and suspension cells, eliminating the need to trypsinize cells and is ideal for a broad range of applications including 3D multicellular tumor spheroids, proliferation, cell line development, colony counting, wound healing, and more.ons.

Ask a Specialist
Request a Quote

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Cellometer Spectrum image cytometer

Advanced imaging with 2 interchangeable fluorescent optics modules for simple, single sample 20µl cell-based assays with flow-like data output.

Cellometer Vision CBA image cytometer

A micro-well, plate-based multi-channel bright field and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells.

Celigo imaging cytometer

Cellometer and Celigo Image Cytometers at a Glance

Cell / Sample Type

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Cell Line X
X
X X X
Cultured Primary Cells X
X
X X X
Algae X
 
X
Platelets X
X
Low Concentration Cell Lines X
X
 
X X X
Yeast (Clean Sample)
X
Yeast (Messy Sample)
X
Primary cells (Messy Sample*) X
X X
PBMCs, Splenocytes, Stem Cells X
X
X X
Hepatocytes X
X
X X
Adipocytes*** X
X
X X X
Cell-Based Assay ** X
X
X
X X X
Apoptosis (Annexin V-FITC/PI) X
X
X
X X
Apoptosis (Caspase Activity) X
X
X
X X
Autophagy (CytoID-green) X
X
X
Cell Proliferation (CFSE) X
X
X
X X
Cell Cycle (PI) X
X
X
X X
GFP Transfection X
X
X
X X
RFP Transfection X
X
X
X X
Mitochondrial Potential (JC-1)
X
X
X X
Multi-drug Resistance (ABC Transporter) X
X
X
X X
Surface Marker Analysis X
X
X
X X
Vitality (Calcein-AM/PI) X
X
X
X X
Image Cytometry** X
X
X
X X

Imaging Capabilities

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Fluorescence Intensity Measurements X
X
X
X X
Detection of Weak Fluorescence X
X
X
X X

Configuration

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Objective Magnification 5X
5X
10X
Bright field X
X
X
X X X
Contains Single Fluorescence Optics Module
Contains Dual Fluorescence Optics Module X
X
X
3 Fluorescent Channels
X
4 Fluorescent Channels
X
Desktop Work Station Included
X X X
Optional Satellite Work Station Available
X X X
User-changeable optics module(s)
X
X

Software Features

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Software for analysis of clumpy and irregular-shaped cells X
X
X
X X X
Mean cell diameter and cell size distribution X
X
X
X X X
Sample adjustment calculator to determine sample volume for desired cell number / concentration X
X
X
Cell type wizard for creating new cell type parameters X
X
X
IQ / OQ Option X
X
X
GLP/GMP Audit Control Option X
X
X
Specialized algorithms for analysis of adipocytes*** X
X
Specialized algorithms for analysis of hepatocytes X
X
FCS Express Software for flow-like data reporting for cell-based assays X
X
X
X X
FCS Express Software for Image Cytometry X
X
X
X X

Performance Criteria

Spectrum

Vision CBA

Vision CBA (10x)

Celigo BF

Celigo 4 Channel

Celigo 5 Channel

Analysis time 5-15 sec.
5-15 sec.
5-15 sec.
Cell size range 5-300µm
5-300µm
0.5-15µm
Sample volume 20 µl
20 µl
20 µl
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell-Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter