//Cellometer and Celigo Image Cytometers
Cellometer and Celigo Image Cytometers 2018-06-26T16:50:12+00:00

Cellometer and Celigo Image Cytometers

Nexcelom’s image cytometers give researchers increased control of their samples and the data generated from their cell-based assays. The Cellometer Vision CBA system requires 20 µL of sample in suspension and is a simple, quick solution for assays such as cell cycle, apoptosis, GFP transfection and surface marker analysis. The Celigo offers a plate-based high-throughput solution for adherent and suspension cells, eliminating the need to trypsinize cells and is ideal for a broad range of applications including 3D multicellular tumor spheroids, proliferation, cell line development, colony counting, wound healing, and more.ons.

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Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Cellometer Spectrum image cytometer

Advanced imaging with 2 interchangeable fluorescent optics modules for simple, single sample 20µl cell-based assays with flow-like data output.

Cellometer Vision CBA image cytometer

A micro-well, plate-based multi-channel bright field and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells.

Celigo imaging cytometer

Cellometer and Celigo Image Cytometers at a Glance

Cell / Sample Type Spectrum Vision CBA Vision CBA (10x) Celigo BF Celigo 4 Channel Celigo 5 Channel
Cell Line
X
X X X
Cultured Primary Cells
X
X X X
Algae
 
X
Platelets
X
Low Concentration Cell Lines
X
 
X X X
Yeast (Clean Sample)
X
Yeast (Messy Sample)
X
Primary cells (Messy Sample*)
X X
PBMCs, Splenocytes, Stem Cells
X
X X
Hepatocytes
X
X X
Adipocytes***
X
X X X
Cell-Based Assay **
X
X
X X X
Apoptosis (Annexin V-FITC/PI)
X
X
X X
Apoptosis (Caspase Activity)
X
X
X X
Autophagy (CytoID-green)
X
X
Cell Proliferation (CFSE)
X
X
X X
Cell Cycle (PI)
X
X
X X
GFP Transfection
X
X
X X
RFP Transfection
X
X
X X
Mitochondrial Potential (JC-1)
X
X
X X
Multi-drug Resistance (ABC Transporter)
X
X
X X
Surface Marker Analysis
X
X
X X
Vitality (Calcein-AM/PI)
X
X
X X
Image Cytometry**
X
X
X X
Imaging Capabilities
Spectrum Vision CBA Vision CBA (10x) Celigo BF Celigo 4 Channel Celigo 5 Channel
Fluorescence Intensity Measurements
X
X
X X
Detection of Weak Fluorescence
X
X
X X
Configuration
Spectrum Vision CBA Vision CBA (10x) Celigo BF Celigo 4 Channel Celigo 5 Channel
Objective Magnification
5X
10X
Bright field
X
X
X X X
Contains Single Fluorescence Optics Module
Contains Dual Fluorescence Optics Module
X
X
3 Fluorescent Channels
X
4 Fluorescent Channels
X
Desktop Work Station Included
X X X
Optional Satellite Work Station Available
X X X
User-changeable optics module(s)
X
X
Software Features
Spectrum Vision CBA Vision CBA (10x) Celigo BF Celigo 4 Channel Celigo 5 Channel
Software for analysis of clumpy and irregular-shaped cells
X
X
X X X
Mean cell diameter and cell size distribution
X
X
X X X
Sample adjustment calculator to determine sample volume for desired cell number / concentration
X
X
Cell type wizard for creating new cell type parameters
X
X
IQ / OQ Option
X
X
GLP/GMP Audit Control Option
X
X
Specialized algorithms for analysis of adipocytes***
X
Specialized algorithms for analysis of hepatocytes
X
FCS Express Software for flow-like data reporting for cell-based assays
X
X
X X
FCS Express Software for Image Cytometry
X
X
X X
Performance Criteria
Spectrum Vision CBA Vision CBA (10x) Celigo BF Celigo 4 Channel Celigo 5 Channel
Analysis time
5-15 sec.
5-15 sec.
Cell size range
5-300µm
0.5-15µm
Sample volume
20 µl
20 µl
*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell-Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter