Cell Cycle Reagents
Principle of the ViaStain™ PI Cell Cycle Assay
Cell cycle analysis is used to determine the proportion of cells in each stage of the cell cycle for a given cell population based on variations in DNA content. In this assay, each cell is stained with propidium iodide (PI), a fluorescent nuclear staining dye that intercalates with DNA. Because the dye cannot enter live cells, the cells are first fixed (with ethanol or methanol), and then stained with the cell cycle reagent. Cells preparing for division will contain increasing amounts of DNA and display proportionally increased fluorescence. Differences in fluorescence intensity are used to determine the percentage of cells in each phase of the cell cycle.
ViaStain™ PI Cell Cycle Kit (for Celigo)
Stain, Incubate, and Image: All within your well – Perfect for multiplexing your assays:
The ViaStain PI Cell Cycle reagent is designed for quantitative analysis of cellular DNA using propidium iodide (PI) on the Celigo Image Cytometer. Ribonuclease A (RNaseA) is included within the solution.
- This cell cycle kit is formulated to work on adherent cell lines. This eliminates trypsinization as well as sample prep for flow cytometry. (Also works with suspension cell lines)
- The reagent already contains RNase and PI at the required concentration for easy cell staining and imaging on the Celigo image cytometer.
- Predefined staining, imaging, and analysis protocols are provided with the kit. This allows for easy execution of the assay.
Whole well image of adherent cells is acquired and each cell in automatically identified by the software. The total fluorescence intensity with each cell is exported to FCS express where the data is analyzed and plotted.
Data in FCS express is gated on single cells and cell cycle histogram is generated using the mathematical model. The mathematical modeling allows for consistent data output for both control and treated samples.
| G1 Mean | G1 CV | % G1 | G2 Mean | G2 CV | % G2 | % S | G2/G1 |
|---|---|---|---|---|---|---|---|
| 9215.18 | 8.90 | 45.76 | 19353.45 | 7.66 | 11.92 | 42.33 | 2.13 |
Adherent Plate-based Cell Cycle with ViaStain using Celigo Cell Cycle PI Reagent
ViaStain™ PI Cell Cycle Kit (for Cellometer K2, Vision CBA, and Spectrum)
The ViaStain™ PI Cell Cycle Kit is designed for fast, simple determination of the cell cycle distribution for a cell population using image cytometry. Each kit contains ready-to-use PI/RNase staining solution sufficient for 100 tests along with a detailed instruction manual outlining cell staining, imaging, and cell cycle data analysis.
| Cell Population | % of Gated Cells | CV | Concentration (10^6 cells/mL) |
|---|---|---|---|
| Total | 100 | 9.2 | |
| Sub G1 | 1.2 | 5.1 | 0.1 |
| G0/G1 | 63.4 | 10.5 | 5.8 |
| S | 13.9 | 11.3 | 1.3 |
| G2/M | 21.0 | 8.2 | 1.9 |