Cell Cycle Reagents
Instrument Compatibility for Cell Cycle Reagents
|Auto 2000||Auto X4||X1||X2||K2||Vision CBA||Celigo|
|ViaStain™ PI Cell Cycle Kit||X||X|
ViaStain™ PI Cell Cycle Kit
The ViaStain™ PI Cell Cycle Kit is designed for fast, simple determination of the cell cycle distribution for a cell population using image cytometry. Each kit contains ready-to-use PI/RNase staining solution sufficient for 100 tests along with a detailed instruction manual outlining cell staining, imaging, and cell cycle data analysis.
|Cell Population||% of Gated Cells||CV||Concentration (10^6 cells/mL)|
Cell cycle histogram (left) and data table (above) for a Jurkat cell control sample.
Principle of the ViaStain™ PI Cell Cycle Assay
Cell cycle analysis is used to determine the proportion of cells in each stage of the cell cycle for a given cell population based on variations in DNA content. In this assay, each cell is stained with propidium iodide (PI), a fluorescent nuclear staining dye that intercalates with DNA. Because the dye cannot enter live cells, the cells are fixed with ethanol or methanol, then all of the cells are stained. Cells preparing for division will contain increasing amounts of DNA and display proportionally increased fluorescence. Differences in fluorescence intensity are used to determine the percentage of cells in each phase of the cell cycle.