Rapid Detection of ABC Transporter Interaction: Potential Utility in Pharmacology
Journal of Pharmacological and Toxicological Methods, November 2010. Robey RW, Lin B, Qiu J, Chan LL, Bates SE
We have developed a method to characterize the function and inhibition of ABC transporters using an automated cell counter with fluorescence detection capability. The assay was performed using stably-transfected HEK293 cells expressing P-gp, MRP1, or ABCG2 and examining transport of fluorescent substrates in the presence or absence of known inhibitors and compared to results obtained with a flow cytometer.