Cellometer Mini Automated Cell Counter

Affordable, accurate automated cell counting using trypan blue viability

Save $500 – Buy Online
Request a Quote

Introduction: Cellometer Mini Bright Field Automated Cell Counter

The Cellometer Mini utilizes bright field imaging and pattern-recognition software to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Trypan blue cell count, concentration, diameter, and % viability are automatically calculated and reported in <10 seconds … in only 3 seconds with some computers!

Fast, Simple, Accurate Analysis

Load Sample

Select Assay, Count

View Image

Insert Slide

Get Results

fl

Brightfield cell counting is recommended only for cell lines or purified/homogenous primary samples. Fluorescence cell counting is recommended for PBMCs, mouse splenocytes, hepatocytes, and other similar primary nucleated cell samples. To see our excellent line of fluorescent cell counters, please visit our fluorescent cell counters page.


Small Footprint, Compatible with Most Computers

The Cellometer Mini is designed to fit in even the smallest lab space. Cellometer software can be loaded on most computers* and linked to the Cellometer Mini with a special USB cable.

*Minimum Recommended Computer Specifications
2.2 GHz or higher
4GB RAM
USB 2.0 port
1024 x 768 pixel display
Windows XP, Vista, Windows 7, or Windows 8

Small computer for trypan blue cell counting

Cellometer Mini software is compatible with touchscreen laptops and computers.


Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cell image for trypan blue cell counting

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the trypan blue cell counted image of A549 cancer cells shown at right.

Irregular-shaped cells - trypan blue cell counting

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at right.


Cell Size Analysis & Size-based Counting

Cell size histogram from trypan blue cell countingThe Cellometer Mini automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.


Counted Cell Images for Data Verification

Counted Cell Images shows live and dead cells during trypan blue cell countingCounted cells can be viewed on-screen for verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • cells are counted correctly, based on size and shape
  • debris is excluded
  • cells within clumps are being counted individually

Faster and More Precise than Manual Cell Counting

Hemacytometer under microscope during trypan blue cell counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Mini reports count and concentration for live and dead cells and % viability in <10 seconds … more than 25 x faster than manual counting!

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Eliminate Recording & Calculation Errors
  • Reduce Inter-Operator Variability
  • Standardize Data Reporting
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer cell counting chambers for trypan blue cell counting

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Easily Print & Save Data and Cell Images

Print

Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the “Auto-Print” setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network, local computer, or data stick. Choose to Auto-Save data, raw images, and/or counted images to a network, local computer, or data stick.


Dedicated On-line and On-site Applications Support

Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Mini Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Cellometer Mini Applications

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Mini Automated Cell Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Mini
Average 1.03E+06 1.04E+06
STDEV 6.60E+04 9.49E+04
%CV 6.4% 9.1%

5 µm beads

N= 20 Hemacytometer Cellometer Mini
Average 1.07E+06 1.12E+06
STDEV 5.90E+04 6.04E+04
%CV 5.5% 5.4%

Cellometer Mini Customer Testimonials

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and usually, it takes a lot of time. I can only count 2 cell lines at a time using a hemocytometer. But using the [Mini] automated cell counter I have been able to count 15 different cell lines in less than 10 minutes.
I absolutely love this product, and recommend it for cell culture use!


Purdue University

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and usually, it takes a lot of time. I can only count 2 cell lines at a time using a hemocytometer. But using the [Mini] automated cell counter I have been able to count 15 different cell lines in less than 10 minutes.
I absolutely love this product, and recommend it for cell culture use!

Purdue University

Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least variance across individuals. Reduction in manual error translates to greater reliability for the results.


Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least variance across individuals. Reduction in manual error translates to greater reliability for the results.

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier especially when working with multiple different cells lines. This is a great machine to train new students and who are new to cell counting.


Craig Sweet

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier especially when working with multiple different cells lines. This is a great machine to train new students and who are new to cell counting.

Craig Sweet

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments.


Walter Reed Army Institute of Research

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments.

Walter Reed Army Institute of Research

Read more testimonials from our customers

Our customers include:

Customer Publications Using Cellometer Mini