Cellometer Mini Automated Cell Counter
Affordable, accurate automated cell counting using trypan blue viability
Introduction: Cellometer Mini Bright Field Automated Cell Counter
The Cellometer Mini utilizes bright field imaging and pattern-recognition software to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Trypan blue cell count, concentration, diameter, and % viability are automatically calculated and reported in <10 seconds … in only 3 seconds with some computers!
Fast, Simple, Accurate Analysis
Select Assay, Count
Small Footprint, Compatible with Most Computers
The Cellometer Mini is designed to fit in even the smallest lab space. Cellometer software can be loaded on most computers* and linked to the Cellometer Mini with a special USB cable.
*Minimum Recommended Computer Specifications
2.2 GHz or higher
USB 2.0 port
1024 x 768 pixel display
Windows XP, Vista, Windows 7, or Windows 8
Cellometer Mini software is compatible with touchscreen laptops and computers.
Analyze Individual Cells in Clusters and Irregular-Shaped Cells
The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the trypan blue cell counted image of A549 cancer cells shown at right.
The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at right.
Cell Size Analysis & Size-based Counting
The Cellometer Mini automatically generates a cell size histogram based on cell diameter.
The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.
Counted Cell Images for Data Verification
Counted cells can be viewed on-screen for verification that cells in the sample are being imaged and analyzed properly.
Users can confirm that:
- cells are counted correctly, based on size and shape
- debris is excluded
- cells within clumps are being counted individually
Faster and More Precise than Manual Cell Counting
Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Mini reports count and concentration for live and dead cells and % viability in <10 seconds … more than 25 x faster than manual counting!
Improve Data Accuracy & Consistency
- Eliminate Wash Steps
- Eliminate Judgment Errors
- Eliminate Recording & Calculation Errors
- Reduce Inter-Operator Variability
- Standardize Data Reporting
- Reduce Counting Time … Run More Experiments
Counting Chambers – No Washing or Contamination
Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.
The disposable Cellometer Cell Counting Chambers offer several key advantages:
- Time savings – no washing
- No risk of cross-contamination
- Reduced biohazard risk to users
- Controlled sample volume
Easily Print & Save Data and Cell Images
Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the “Auto-Print” setting to automatically generate a report for each cell count.
Easily Save data and images to a network, local computer, or data stick. Choose to Auto-Save data, raw images, and/or counted images to a network, local computer, or data stick.
Dedicated On-line and On-site Applications Support
Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:
- Creation of new cell types
- Optimization of counting parameters
- Training of new users
- Installation of a new Cellometer Mini Cell Counter
All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or firstname.lastname@example.org
Cellometer Mini Applications
Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »
When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »
Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »
Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »
Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »
Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »
Performance of the Cellometer Mini Automated Cell Counter
Consistency and Accuracy Comparison to Hemacytometer
|N = 20||Hemacytometer||Cellometer Mini|
5 µm beads
|N= 20||Hemacytometer||Cellometer Mini|