Cellometer Mini Automated Cell Counter

Affordable, accurate automated cell counting using trypan blue viability

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15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and u...

more
Purdue University
Oct 25, 2018

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and usually, it takes a lot of time. I can only count 2 cell lines at a time using a hemocytometer. But using the [Mini] automated cell counter I have been able to count 15 different cell lines in less than 10 minutes.
I absolutely love this product, and recommend it for cell culture use!
Purdue University
Oct 25, 2018

Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least...

more
Sep 19, 2018

Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least variance across individuals. Reduction in manual error translates to greater reliability for the results.
Sep 19, 2018

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier ...

more
Sep 14, 2018

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier especially when working with multiple different cells lines. This is a great machine to train new students and who are new to cell counting.
Sep 14, 2018

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments....

Walter Reed Army Institute of Research
Jun 27, 2018

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments.
Walter Reed Army Institute of Research
Jun 27, 2018

Introduction: Cellometer Mini Bright Field Automated Cell Counter

The Cellometer Mini utilizes bright field imaging and pattern-recognition software to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Trypan blue cell count, concentration, diameter, and % viability are automatically calculated and reported in <10 seconds … in only 3 seconds with some computers!

Fast, Simple, Accurate Analysis

Load Sample

Loading Sample for trypan blue cell counting

Insert Slide

Inserting Slide for trypan blue cell counting

Select Assay, Count

Select Assay for trypan blue cell counting

View Images

View counted images for trypan blue cell counting

Get Results

Get Results from trypan blue cell counting


Small Footprint, Compatible with Most Computers

The Cellometer Mini is designed to fit in even the smallest lab space. Cellometer software can be loaded on most computers* and linked to the Cellometer Mini with a special USB cable.

*Minimum Recommended Computer Specifications
2.2 GHz or higher
4GB RAM
USB 2.0 port
1024 x 768 pixel display
Windows XP, Vista, Windows 7, or Windows 8

Small computer for trypan blue cell counting

Cellometer Mini software is compatible with touchscreen laptops and computers.


Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cell image for trypan blue cell counting

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the trypan blue cell counted image of A549 cancer cells shown at right.

Irregular-shaped cells - trypan blue cell counting

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at right.


Cell Size Analysis & Size-based Counting

Cell size histogram from trypan blue cell countingThe Cellometer Mini automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.


Counted Cell Images for Data Verification

Counted Cell Images shows live and dead cells during trypan blue cell countingCounted cells can be viewed on-screen for verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • cells are counted correctly, based on size and shape
  • debris is excluded
  • cells within clumps are being counted individually

Faster and More Precise than Manual Cell Counting

Hemacytometer under microscope during trypan blue cell counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Mini reports count and concentration for live and dead cells and % viability in <10 seconds … more than 25 x faster than manual counting!

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Eliminate Recording & Calculation Errors
  • Reduce Inter-Operator Variability
  • Standardize Data Reporting
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer cell counting chambers for trypan blue cell counting

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Easily Print & Save Data and Cell Images

Print

Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the “Auto-Print” setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network, local computer, or data stick. Choose to Auto-Save data, raw images, and/or counted images to a network, local computer, or data stick.


Dedicated On-line and On-site Applications Support

Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Mini Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Cellometer Mini Applications

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Mini Automated Cell Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Mini
Average 1.03E+06 1.04E+06
STDEV 6.60E+04 9.49E+04
%CV 6.4% 9.1%

5 µm beads

N= 20 Hemacytometer Cellometer Mini
Average 1.07E+06 1.12E+06
STDEV 5.90E+04 6.04E+04
%CV 5.5% 5.4%

Cellometer Mini Customer Testimonials

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and u...

more
Purdue University
Oct 25, 2018

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and usually, it takes a lot of time. I can only count 2 cell lines at a time using a hemocytometer. But using the [Mini] automated cell counter I have been able to count 15 different cell lines in less than 10 minutes.
I absolutely love this product, and recommend it for cell culture use!
Purdue University
Oct 25, 2018

Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least...

more
Sep 19, 2018

Consistent results

It [Cellometer Mini] is a faster, higher throughput way of getting consistent results with the least variance across individuals. Reduction in manual error translates to greater reliability for the results.
Sep 19, 2018

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier ...

more
Sep 14, 2018

Great machine to train new students

I have used the Cellometer Mini in my lab for about 6 months and my workload has become much easier especially when working with multiple different cells lines. This is a great machine to train new students and who are new to cell counting.
Sep 14, 2018

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments....

Walter Reed Army Institute of Research
Jun 27, 2018

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments.
Walter Reed Army Institute of Research
Jun 27, 2018

More of what our customers have to say about Nexcelom products –

Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great....

Feb 25, 2019

Would definitely recommend

Cellometer Auto T4 has made my life easy. Now I don't have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. Cellometer is very easy to use, fast and accurate. The best thing is that I can optimize the settings for counting different cells on the basis ...

Dec 10, 2018

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we used to do to count our cells. We count everything from CHO-k1, PMBCs, and mast cells using this machine. We particularly find this method of counting cells is far superior to any other method we have tr...

Dec 10, 2018

I was using a hemocytometer…

I was using a hemocytometer...the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images....

Aurora Fabry-Wood
Dec 7, 2018

Utmost confidence

Using the X2 has revolutionized our ability to monitor our overall yeast health and performance. Allowing us to accurately harvest and re-pitch our yeast strains for successive generations with the utmost confidence....

Dec 5, 2018

Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable option for the precision being offered. This instrument is a great addition to any lab!...

Dec 5, 2018

This machine has transformed the way I culture

This machine [Cellometer K2] has transformed the way I culture. No longer do I have to spend hours out of my day to manually could cells. It has changed the way I see cell culture from a menial chore to something I look forward to every day. Cheers NEXCELOM for a job well done!!!!...

Nov 29, 2018

Very easy to use

The Auto T4 is very easy to use and the software is very friendly. I save a lot of time when I count number of cells and more when I am using more than one cell line....

Nov 29, 2018

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a daily basis. We mainly use it to count live or dead cells in a population and to maintain viable levels of NK92mi cells in the desired concentration range....

Nov 27, 2018

Allowed us to enter a new field of cell culture

The K2 has allowed us to enter a new field of cell culture with best practices already in place. Not having to optimize the use of a cell counter will save us a lot of time....

Nov 21, 2018

The customer service is good too

We use [Cellometer] to count PBMC and tumor cells, Auto T4 is easy and good to use for measuring. The customer service is good too, willing to come and help set the optimization of the instrument. We recommend not only these machines, but this company also....

Nov 14, 2018

An awesome cell counter

I use the Auto 2000. It is an awesome cell counter. Very easy to use and fast. My cell counts are more accurate with the Cellometer compared to other cell counters. I also found the webinars and training/support very accessible and helpful....

Nov 12, 2018

Very accurate, reliable, and easy to use

The Cellometer Auto 2000 has completely replaced the Vi-Cell and Countess in my workflow. I primarily use it for counting and checking the PBMCviability of primary cells and PBMCs using the AO/PI function. I have found it to be very accurate, reliable, and easy to use....

Nov 8, 2018

Very good alternative to flow cytometer

Cellometer K2 is very compact, reliable and easy to use. Our lab uses this counter to count live and dead cells for our experiments. It is a very good alternative to flow cytometer for cell cycle and apoptosis studies....

Stanford University
Oct 26, 2018

15 different cell lines in less than 10 minutes

This product is such a time saver!
I do experiments involving many different cell lines and usually, it takes a lot of time. I can only count 2 cell lines at a time using a hemocytometer. But using the [Mini] automated cell counter I have been able to count 15 different cell lines in less tha...

Purdue University
Oct 25, 2018

Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks, as well as contributing to our research studies. It is very straightforward to use....

Dec 11, 2018

Reproducible results

In comparison to the standard method for manual cell counting, Cellometer K2 helps me in counting cells efficiently and quickly with reproducible results. I use it mainly for assessing cell vitality by trypan blue exclusion assay....

Dec 11, 2018

Great

It is easy to operate. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. It also provides cell sizes and distribution that are very informative to help you know your cells better. This instrument is alr...

Dec 12, 2018

A welcome addition

The Nexcelom [Cellometer] X2 unit we received is a welcome addition to our microbiology lab. It was very easy to set up, and a tech came out and walked us through proper usage, and set up assays for our house yeast strains. We are very happy to not have to rely on a hemocytometer and time-consuming ...

Zachary Foster
Feb 25, 2019

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality....

Feb 25, 2019

Highly recommended from a colleague

The Auto T4 Cellometer is easy to set up and intuitive to use. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. This counter came highly recommended from a colleague and so we had to get one for ourselves. It has made counting cells far m...

Feb 25, 2019

A time-saving machine

Cellometer [T4] has greatly reduced the time it takes to count the cells. There is a large library of settings for different type of cells. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine....

Jan 17, 2019

Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a lot of valuable time that we now can dedicate to other work. We love the Auto 2000!...

Jan 17, 2019

User-friendly

Good Purchase. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly....

Jan 10, 2019

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