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  5. Assay Protocol: Automated (Auto T4) vs. Manual Cell Counting

For researchers performing manual cell counting, a key factor involved in evaluation of an automated cell counter is comparison to their current method. Based on our experience supporting thousands of Cellometer Auto T4 customers in the field, we have designed the following protocol to make comparison of the Cellometer Auto T4 Cell Counter to manual hemacytometer results as accurate as possible. For the comparison to be valid, Auto T4 and hemacytometer counts must be done at the same time using aliquots from the same cell sample.

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