I cannot see anything in the fluorescent channel images. They are completely black/green, what’s happening?
If you cannot see anything in the fluorescent channels, first check the Assay Editor to make sure the correct lamps/modules are selected. Make sure the exposure time is set appropriately. If you see images that are too bright, reduce the exposure time incrementally until the ideal setting is found. If you have a sample of the fluorescent beads (shipped with all fluorescent instruments), prepare a slide of the beads. This is useful for confirming the instrument channels are working appropriately.