ViaStain™ CMFDA (5-Chloromethylfluorescein diacetate) is chemically same molecule as CellTracker™ Green CMFDA (trademark of Invitrogen). CMFDA is a cell-permeable, non-fluorescent compound. Upon crossing the cell membrane, it is rapidly hydrolyzed by cellular esterases inside live cells converting it from a non-fluorescent to a strongly green fluorescing dye. Upon hydrolysis, the more hydrophilic CMFDA is trapped inside the cell until cell division. Upon cell division, approximately half of the fluorescent label is passed onto the daughter cell. After each successive cell division, the fluorescent intensity of the dye is reduced by half and the staining appears proportionately dimmer. The dye becomes difficult to detect after approximately 3-5 generations.
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