Why do you suggest a 0.1% trypan blue final concentration on the Cellometer Auto T4?
Any higher concentration (0.2% and greater) can cause cell viability to decrease quicker, as a result of the cytotoxicity of the dye. Also, higher concentrations of trypan blue also causes a darker background image that can give viable cells the appearance of dead cells. 0.1% trypan blue will still stain the dead cells very clearly.