The Institute of Clinical Biochemistry (Hanover, Germany) hypothesized that the pro-inflammatory cytokine environment seen in obese patients and those with obesity-related diabetes promotes the dysregulation of brown adipose tissues (BAT), which in turn intensifies diabetes progression. With a murine non-differentiated brown adipocyte cell line, researchers examined how exposure to pro-inflammatory cytokines impacted these cells. Cell density calculations were performed on the Cellometer Auto T4. The pro-inflammatory cytokines negatively impacted the cells’ viability, markedly increased reactive oxygen species production, and down-regulated markers specific to BAT such as UCP-1 and β-Klotho. The scientists concluded that pro-inflammatory cytokines lead to BAT death and dysfunction, as well as an increase in oxidative stress.
Cellometer Auto T4 investigates dysfunction to pro-inflammatory cytokine toxicity and reactive oxygen species
By Polly Mitchell|2021-06-15T19:11:35+00:00March 1st, 2016|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: adipocytes, Cellometer Auto T4, cytokines, diabetes|0 Comments
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