Whole-well imaging analysis for quality control of cell-based assays in AAV vector design
Extensive cell characterization is critical for gene therapy research and development. Accurate and precise cell characterization is necessary for optimizing experimental conditions, manufacturing regulation, and eventual product release. To ensure precise results are produced, cell-based assay conditions must have quality control.
The AAV vector development process is complex and tedious (Figure 1). To accurately perform high-quality cell-based assays many factors must be optimized to avoid end-product variations including host-cell quality, quantity, viability, passage number, and cell preparation procedures.
What parameters should be monitored for optimizing the process to produce AAV2-transgene vectors?