Channou Aing, Author at Nexcelom Bioscience

Poster: Cellometer for Autophagy Detection in Living Cells

Download the Poster A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells Leo L. Chan, Dee Shen, Alisha R. Wilkinson, Wayne Patton, Ning Lai, Eric Chan, Dmitry Kuksin, Bo Lin, and Jean Qiu Autophagy is an important cellular catabolic process that plays a variety of important roles, including maintenance of the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearance of intracellular microbes. Currently employed autophagy detection methods include fluorescence microscopy, biochemical measurement, SDS-PAGE, and Western blotting, but they are time-consuming, labor-intensive, and require much experience for accurate interpretation. More recently, development of novel [...]

By Channou Aing|2021-03-31T01:59:48+00:00March 31st, 2021|0 Comments

Poster: Using Cellometer to Eliminate RBC-Induced Counting Error

Download the Poster Accurate PBMC Concentration Measurement using Image Cytometry to Eliminate RBC-Induced Counting Error Leo L. Chan, Daniel J. Laverty, Tim Smith, Parham Nejad, Hillary Hei, Roopali Gandhi, Dmitry Kuksin, and Jean Qiu Peripheral blood mononuclear cells (PBMCs) have been widely researched in the fields of immunology, infectious disease, oncology, transplantation, hematological malignancy, and vaccine development. Specifically, in immunology research, PBMCs have been utilized to monitor concentration, viability, proliferation, and cytokine production from immune cells, which are critical for both clinical trials and biomedical research. The viability and concentration of isolated PBMCs are traditionally measured by manual counting with [...]

By Channou Aing|2021-03-31T01:54:33+00:00March 31st, 2021|0 Comments

Poster: Cellometer for detection of physiological and metabolic changes in Saccharomyces cerevisiae

Download the Poster Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiae Leo L. Chan, Alexandria L. Kury, Alisha Wilkinson, Charlotte Berkes, Alnoor Pirani The study and monitoring of physiological and metabolic changes in Saccharomyces cerevisiae (S. cerevisiae) has been a key research area for the brewing, baking and biofuels industries, which rely on these economically important yeasts to produce their products. Specifically for breweries, physiological and metabolic parameters such as viability, vitality, glycogen, neutral lipid, and trehalose content can be measured to better understand the status of S. cerevisiae during fermentation. Traditionally, these physiological [...]

By Channou Aing|2021-03-31T01:50:37+00:00March 31st, 2021|0 Comments

Poster: Cellometer Analysis Tool for Visual Verification of Gated Cell Populations

Download the Poster Cellometer Image Cytometry as a Complementary Analysis Tool to Flow Cytometry for Visual Verification of Gated Cell Populations Dmitry Kuksin, Christina Arieta Kuksin, Leo L. Chan, Bo Lin, Jean Qiu, and Peter Y. Li Traditionally, many cell-based assays that analyze cell populations and functionalities have been performed using flow cytometry. However, flow cytometers remain relatively expensive, and require highly trained operators for routine maintenance and data analysis. Flow cytometer can process and generate a large number of events, but the data gating strategies are often complex and are performed without the visual confirmation of the cells processed, [...]

By Channou Aing|2021-03-31T01:47:48+00:00March 31st, 2021|0 Comments

Poster: Automated Quantification of Budding Saccharomyces cerevisiae using Cellometer

Download the Poster Automated Quantification of Budding Saccharomyces cerevisiae using a Novel Image Cytometry Method Leo L. Chan, Daniel J. Laverty, Alexandria L. Kury, Dmitry Kuksin, Alnoor Pirani, and Kevin Flanagan The measurements of concentration, viability, and budding percentages of Saccharomyces cerevisiae are performed on a routine basis in the biofuel and brewing industries. Generation of these parameters is of great importance in a manufacturing setting, where they can aid in the estimation of product quality, quantity, and fermentation time of the manufacturing process. Specifically, budding percentages can be used to estimate the reproduction rate of yeast populations, which directly [...]

By Channou Aing|2021-03-31T01:46:55+00:00March 31st, 2021|0 Comments

Poster: Cellometer comparison of trypan blue and fluorescence-based cellular viability

Download the Poster Morphological observations using image cytometry for the comparison of trypan blue and fluorescence-based cellular viability Leo L. Chan, Dmitry Kuksin, Daniel J. Laverty, Jean Qiu, and Bo Lin Determining cell viability is an essential component in many biological experiments ranging from standard cell culture to immunology and oncology research. The traditional method for cell viability measurement is the trypan blue exclusion assay, which utilizes a hemacytometer to manually enumerate trypan blue stained cells. However, trypan blue exclusion assays canbe time-consuming, tedious, and have user-dependent variation. Studies have also shown that long incubation with trypan blue can induce [...]

By Channou Aing|2021-03-31T01:39:32+00:00March 31st, 2021|0 Comments

Poster: GFP Reporters in Primary Articular Chondrocyte Cultures using Cellometer

Download the Poster Discriminating Multiplexed GFP Reporters in Primary Articular Chondrocyte Cultures using Cellometer Image Cytometry Leo L. Chan, Jianping Huang, Yusuke Hagiwara, Leonardo Aguila, and David Rowe Flow cytometry has become a standard tool for defining a heterogeneous cell population based on surface expressed epitopes or GFP reporters that reflect cell types or cellular differentiation. The introduction of image cytometry raised the possibility of adaptation to discriminate GFP reporters used to appreciate cell heterogeneity within the skeletal lineages. The optical filtersand LEDs were optimized for the reporters used in transgenic mice expressing various fluorescent proteins. In addition, the need [...]

By Channou Aing|2021-03-31T01:34:50+00:00March 31st, 2021|0 Comments

Poster: Automation Method to Increase Efficiency in Cell Line Development

Download the Poster Automation Method to Increase Efficiency in Cell Line Development Sarah Kessel, Leo L. Chan, and Olivier Dery Development of monoclonal cell lines is essential in research and for the production of recombinant protein therapeutics. Due to the increasing number of biologic treatments, monoclonal antibody (mAb) producing cell lines are under increasing regulatory scrutiny, and therefore, ensuring monoclonality is becoming an essential step in the cell line development process. Some challenges in producing clones remain and relate to cell plating, identification and outgrowth of single cells for monoclonality.Researchers have used limiting dilution techniques and microscope visualization to ensure [...]

By Channou Aing|2021-03-31T01:31:34+00:00March 31st, 2021|0 Comments

Poster: Cellometer Analysis of Fluorescent Viability and Vitality Staining Methods for Saccharomyces Cerevisiae

Download the Poster Image-Based Cytometric Analysis of Fluorescent Viability and Vitality Staining Methods for Saccharomyces Cerevisiae Leo L. Chan, Dan Driscoll, Stephanie Saldi, and Dmitry Kuksin Saccharomyces cerevisiae has been an essential component for the production of beer for centuries. The viability and vitality of yeast during standard brewing process is especially important for proper cell growth,consistent production of flavor, and optimal yield for fermentation. Viability refers to the ability of the yeast to live and continue dividing, while the vitality refers to the metabolic activity of the yeast. Yeast may be viable and dividing, while not vital and allowing [...]

By Channou Aing|2021-03-31T01:27:08+00:00March 31st, 2021|0 Comments

Poster: Cellometer for Measuring Concentration and Viability of Primary Cells used in Cellular Therapy

Download the Poster Rapid Image Cytometry Method for Measuring Concentration and Viability of Primary Cells used in Cellular Therapy Leo L. Chan, James Lee, Dmitry Kuksin, Afshin Shirazian, and Jean Qiu Cellular therapy has become a major clinical research field that creates tailor-made medical treatments for many human diseases. Primary cells obtained from patients and mouse models often contain nonspecific particles such as red blood cells (RBC), platelets, and cellular debris, which canmake the cell sample analysis difficult. To remove nonspecific particles that can interfere with analysis, a ficoll gradient separation or RBC lysis is routinely performed. Measurements of concentration [...]

By Channou Aing|2021-03-31T01:26:51+00:00March 31st, 2021|0 Comments

About Channou Aing