Channou Aing, Author at Nexcelom Bioscience

Poster: Automated Method to Determine Infectious Dose (TCID50)

Download the Poster Automated Method to Determine Infectious Dose (TCID50) Andrea Murphy PhD, and Catherine Swingler PhD Qualitative assessment of pathogenic infections, as well as the protective effects of candidate therapeutics, often relies on the observation of specificpathological changes within the host cell. These phenotypic changes, such ascell rounding, swelling/shrinking, granularity, etc., are known as a CytopathicEffect (CPE) and can be visualized via light microscopy. As the magnitude and localization of the CPE may vary considerably, careful examination of replicate samples at various titers is required for reliable, qualitative results. This subjective approach which is specific to the infectious agent [...]

By Channou Aing|2021-03-31T01:20:49+00:00March 31st, 2021|0 Comments

Poster: Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method

Download the Poster Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method Sarah Kessel, Eric Sincoff, and Olivier Dery Inhibition of cancer cell proliferation in drug discovery research has not translated well from traditional two-dimensional (2D) in vitro assays to in vivo studies. Most anti-cancer drug compound studies are performed in a tissue culture treated 2D assay format for the purpose of studying proliferation, viability, and apoptosis. Increasingly, scientific evidence is showing that growing cancer cells in the form ofthree-dimensional (3D) spheroids is more predictive of in vivo study outcomes than 2D cell culture formats. Typically, spheroids are created [...]

By Channou Aing|2021-03-31T01:47:27+00:00March 31st, 2021|0 Comments

Poster: Count and quantify fluorescent iPS colonies during 2° reprogramming using Celigo

Download the Poster Non-disruptively count and quantify fluorescent iPS colonies during 2° reprogramming: 7 min per 6-well plate, dual-fluorescence whole well imaging cytometry Scott Cribbes, Sarah Brightwell, and K Kaji Current methodologies for the detection of inducible Pluripotent Stem (iPS)reprogramming are either disruptive e.g. flow cytometry (FC) or low in throughput e.g. fluorescent microscopy (FM). Using the Celigo S Imaging Cytometer and secondary iPS reprogramming we have developed a methodology that combines the advantages of both flow cytometry and fluorescent microscopy. This approach is based on thefluorescent identification of iPS colonies that express the four reprogramming factors, Oct4, Sox2, Klf4 [...]

By Channou Aing|2021-03-31T01:14:43+00:00March 31st, 2021|0 Comments

Poster: Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Celigo

Download the Poster Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee Cytotoxicity assays play a central role in studying the function of immune effector cells such as cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Traditionally, cytotoxicity assays have been performed using51Chromium (51Cr) and Calcein release assays. The assays involve labeling tumor cells (target) with radioisotope or fluorescent dyes, when the target cells are subjected to cytolysis by CTLs or NK cells (effector), they releases the entrapped labels into the [...]

By Channou Aing|2021-03-31T01:11:28+00:00March 31st, 2021|0 Comments

Poster: Antitumor Effect on Ovarian Cancer Cells using Cellometer Imaging Cytometry

Download the Posterhttps://go.nexcelom.com/poster-anti-tumor-effect-ovarian-cancer-cells Measuring Antitumor Effect of c-Myc-Max heterodimerizationinhibitor 100258-F4 on Ovarian Cancer Cells using Cellometer Imaging Cytometry Leo L. Chan, Jiandong Wang, Xiaoli Ma, Hannah M. Jones, Fang Song, Weiyuan Zhang, Victoria L. Bae-Jump, and Chunxiao Zhou Epithelial ovarian carcinoma is the most lethal gynecological cancer due to its silent onset and recurrence with resistance to chemotherapy. Overexpression of oncogene c-Myc is one of the most frequently encountered events present in ovarian carcinoma. Disrupting the function of c-Myc and its downstream target genes is a promising strategy for cancer therapy. In this work, we aimed to evaluate the potential [...]

By Channou Aing|2021-03-31T01:08:48+00:00March 31st, 2021|0 Comments

Poster: Quantification of Natural Killer Cell-Mediated Cytotoxicity using Celigo

Download the Poster Quantification of Natural Killer Cell-Mediated Cytotoxicity using Celigo Imaging Cytometry Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee Cytotoxicity assays play a central role in studying the function of immune effector cells such as cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Traditionally, cytotoxicity assays have been performed using51Chromium (51Cr) and Calcein release assays. The assays involve labeling tumor cells (target) with radioisotope or fluorescent dyes, when the target cells are subjected to cytolysis by CTLs or NK cells (effector), they release the entrapped labels into the media upon lysis. The [...]

By Channou Aing|2021-03-31T01:05:48+00:00March 31st, 2021|0 Comments

Poster: A rapid 3D tumor spheroid analysis method using the Celigo Imaging Cytometry

Download the Poster A rapid 3D tumor spheroid analysis method using the Celigo Imaging Cytometry Leo L. Chan, Scott Cribbes, Sarah Kessel, Olivier Dery, Catherine Swingler, Dmitry Kuksin, Tim Smith, Jean Qiu, Maria Vinci, Lisa Patterson, and Sue Eccles The current 2D methods for cancer drug discovery have had some difficulty in identifying potential drug candidates that can be used for clinical testing. To overcome this challenge, there has been an increase in research of 3D tissue culture, which has facilitated the development of new in-vitro tumor model assays. Traditional 2D and 3D analysis method relied heavily on visual observation [...]

By Channou Aing|2021-03-31T01:02:58+00:00March 31st, 2021|0 Comments

Poster: Cellometer for Quantification of Canine Stromal Vascular Fraction Cells

Download the Poster A Rapid Image Cytometry Method for Quantification of Canine Stromal Vascular Fraction Cells Leo L. Chan, Donald A. Cohen, Dmitry Kuksin, Benjamin D. Paradis, and Jean Qiu In recent years, the lipoaspirate collected from adipose tissue has been seen as a valuable source of adipose-derived mesenchymal stem cells for autologous cellular therapy. For multiple applications, adipose-derived mesenchymal stem cells are isolated from the stromal vascular fraction (SVF) of adipose tissue. Because the fresh stromal vascular fraction typically contains a heterogeneous mixture of cells, determining cell concentration and viability is a crucial step in preparing fraction samples for [...]

By Channou Aing|2021-03-31T00:59:44+00:00March 31st, 2021|0 Comments

Poster: Celigo for Validation and Monitoring of 2° reprogrammed iPSC Colonies

Download the Poster A Rapid Fluorescent Image Cytometry Method for Validation and Monitoring of 2° reprogrammed iPSC Colonies Leo L. Chan, Scott Cribbes, Sara Brightwell, and Keisuke Kaji Flow cytometry (FC) and fluorescent microscopy (FM) have been commonly used for the detection of induced Pluripotent Stem Cell (iPSC) reprogramming, which often have limitations, where flow cytometry requires disruption of adherent iPSCs by trypsinization, and fluorescent microscopy requires manual qualitative analysis that is low throughput. In the recent years, image-based cytometry systems have been utilized to perform direct whole well cell-based assays in microplates without trypsinization and with comparable sensitivity as [...]

By Channou Aing|2021-03-31T00:56:49+00:00March 31st, 2021|0 Comments

Poster: A rapid image cytometric analysis method for phagocytosis using Celigo Imaging Cytometer

Download the Poster A rapid image cytometric analysis method for phagocytosis using Celigo Imaging Cytometer Leo L. Chan, Scott Cribbes, Sarah Kessel, Olivier Dery, Dmitry Kuksin, and Jean Qiu Phagocytosis is an essential process of the immune system to eliminate cellular debris and pathogens. It is a specific form of endocytosis involving vesicular internalization. Phagocytic cells such as macrophages can beattracted to pathogens or cellular debris and engulf the material to be trapped in an internal vesicle called phagosome. The phagosome would fuse with the lysosome to form phagolysosome, where enzymes and toxicperoxides can digest the pathogen or cellular debris [...]

By Channou Aing|2021-03-31T00:53:50+00:00March 31st, 2021|0 Comments

About Channou Aing