Download the Application Note Cellometer® K2 Flourescence Cell Counter & HIVE™ scRNAseq Solution There is a fine line between liberating single cells from their sample matrix and compromising cell integrity. Single cell RNAseq is more sensitive to sample preparation and storage than traditional sequencing, so even seasoned researchers may underestimate the importance of QC for cell viability and sample purity. The Cellometer® K2 Fluorescent Cell Counter measures viability and purity more reliably than trypan blue1,2 and brightfield,3 so you can quickly optimize your sample prep without wasting your sequencing budget. Using the Cellometer® K2 Cell Counter to count cells optimizes [...]
Poster: Cell identity, count, and viability for critical quality attributes using the Cellaca® PLX image cytometer
Cell identity, count, and viability for critical quality attributes using the Cellaca® PLX image cytometer Carolina Franco Nitta, Mackenzie Pierce, Tim Smith, Marek Dobrowolski,Srinivas Gundimeda, Bo Lin, Dmitry Kuksin, and Leo Li-Ying Chan Cell and Gene therapy is one of the fastest growing fields of cancertherapeutics that heavily relies on robust and consistentinstrumentations and technologies to verify and validate fit-for purpose critical quality attributes (CQA). Some of the keyparameters required for satisfying CMC (Chemistry Manufacturingand Controls) criteria standards for cellular therapeutic products arecell identity, count, viability, purity, potency, stability, andmicrobiological quality, which may be routinely performed usingflow cytometry. Some of [...]
Download the Flyer Flyer: Cell Counting - Are You Using the Right Method? Brightfield vs Fluorescence. Fit-for-purpose methodologies are critical to researchers seeking accurate results. Inaccurate results fromchoosing the wrong cell counting method can lead to consequences such as lower quality of products, rejectedpublications, repeated experiments, and lost time. U.S. Congress as well as the National Institute of Standards and Technology (NIST), and other stakeholders, haveidentified that cell counting and viability measurement assurance are important standards for improving thequality of cell therapy products.
Cell-based treatments are life-saving therapies with the potential to offer cures for cancers and other diseases.
Poster: High-throughput chemotherapeutic drug screening of tumor spheroids with individual spheroid results using image cytometry
Download the Poster High-throughput chemotherapeutic drug screening of tumor spheroids with individual spheroid results using image cytometry Jordan Bell, Shilpaa Mukundan, Matthew Teryek, Bo Lin, Biju Parekkadan, Sun Yung, Suzanne Riches, and Leo Li-Ying ChanThree-dimensional cancer models have gained popularity for in vitro studies of chemotherapeutic compounds by providing a more physiologically relevant analog of gas, nutrient, and drug diffusion throughout the tumor microenvironment. Some 3D assays are performed to study individual spheroids over time, where most of these assays rely on maintaining a single spheroid in each well of a 96-well round-bottom ultra-low attachment plate, limiting the number of [...]