About Jeff Ellis

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So far Jeff Ellis has created 4 blog entries.

App Note: Rapid cell count and intactness assessment of pre- and post-bead Ruptor Elite Bead Mill Homogenized neuroprogenitor cells using the Cellometer K2 cell counter

Download the App Note Proper cell sample preparation is a critical step for any cell-based assay. Accurate cell count and viability results are required to prepare cells for downstream assays, and for analysis of intracellular analytes such as DNA/RNA, intracellular proteins, and small molecules, an appropriate cell lysis procedure is needed. Traditional cellular lysis methods can be time-consuming and heavily reliant on reagents such as enzymes or detergents. Download our application note to learn how the Cellometer® K2 Fluorescent Cell Counter was used to accurately determine cell count and viability of neuroprogenitor cells pre-and post-homogenization with the Bead Ruptor Elite Bead [...]

By |2023-07-10T17:41:03+00:00July 7th, 2023|0 Comments

eBook: 10 Key Cell Counting Considerations

Download the eBook As cell and gene therapies have progressed in the last decade, the quality requirements for cell samples have increased because they are involved directly with patient treatment. This necessitates the replacement of traditional cell counting methods with modern, high quality alternatives.Cell counting is crucial for products reviewed by multiple FDA centers, as well as all phases of cell and gene therapy and regenerative medicine drug evaluation. Modernization of cell counting measurements is critical to support the development and manufacturing of these advanced biological products.This Ebook provides best practices for selecting, comparing, and evaluating cell counting methods as [...]

By |2023-07-07T17:12:54+00:00June 15th, 2023|0 Comments

Cellaca PLX Quick Start Guide

Download the Guide Quick Start Guide: Cellaca PLX Instrument must be placed on a level surface and plugged directly into apower outlet. Use of a surge protector is recommended. Ensure all cablesare free from tangles prior to powering on the Cellaca PLX. Follow all equipment safety protocols and keep the area around instrumentclean both during and post operation. Do not position the device so that it isdifficult to disconnect from power main.

By |2023-05-24T19:03:33+00:00May 24th, 2023|0 Comments

Cellaca PLX User Manual

Download the Manual User Manual: Cellaca PLX The Cellaca® PLX Image Cytometry System with Matrix software provides a benchtop solution for performingsimple yet sensitive cell counts, viability readouts and multiplex fluorescence cell-based assay analysis in seconds.Pre-defined assays and report templates, together with dedicated reagents and consumables, enable flow cytometry-like data analysis with no need for titrations, extra incubations, washes or decontamination.

By |2023-05-24T19:03:53+00:00May 24th, 2023|0 Comments

App Note: Best Practices for scRNAseq – Reducing your scRNAseq Sequencing Costs

Download the Application Note Cellometer® K2 Flourescence Cell Counter & HIVE™ scRNAseq Solution There is a fine line between liberating single cells from their sample matrix and compromising cell integrity. Single cell RNAseq is more sensitive to sample preparation and storage than traditional sequencing, so even seasoned researchers may underestimate the importance of QC for cell viability and sample purity. The Cellometer® K2 Fluorescent Cell Counter measures viability and purity more reliably than trypan blue1,2 and brightfield,3 so you can quickly optimize your sample prep without wasting your sequencing budget. Using the Cellometer® K2 Cell Counter to count cells optimizes [...]

By |2022-11-15T22:58:14+00:00November 15th, 2022|0 Comments

Cellaca: Brightfield vs Fluorescence Cell Counting

Download the Flyer Flyer: Cell Counting - Are You Using the Right Method? Brightfield vs Fluorescence. Fit-for-purpose methodologies are critical to researchers seeking accurate results. Inaccurate results fromchoosing the wrong cell counting method can lead to consequences such as lower quality of products, rejectedpublications, repeated experiments, and lost time. U.S. Congress as well as the National Institute of Standards and Technology (NIST), and other stakeholders, haveidentified that cell counting and viability measurement assurance are important standards for improving thequality of cell therapy products.

By |2022-07-12T15:15:16+00:00July 11th, 2022|0 Comments

T cell immunotherapy workflow improvements for clinical-grade manufacturing

Cell-based treatments are life-saving therapies with the potential to offer cures for cancers and other diseases.

Poster: High-throughput chemotherapeutic drug screening of tumor spheroids with individual spheroid results using image cytometry

Download the Poster High-throughput chemotherapeutic drug screening of tumor spheroids with individual spheroid results using image cytometry Jordan Bell, Shilpaa Mukundan, Matthew Teryek, Bo Lin, Biju Parekkadan, Sun Yung, Suzanne Riches, and Leo Li-Ying ChanThree-dimensional cancer models have gained popularity for in vitro studies of chemotherapeutic compounds by providing a more physiologically relevant analog of gas, nutrient, and drug diffusion throughout the tumor microenvironment. Some 3D assays are performed to study individual spheroids over time, where most of these assays rely on maintaining a single spheroid in each well of a 96-well round-bottom ultra-low attachment plate, limiting the number of [...]

By |2023-01-03T13:51:20+00:00May 16th, 2022|0 Comments
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