Polly Mitchell, Author at Nexcelom Bioscience

Product Insert: ViaStain PI

Download the Product Insert PI (propidium iodide) staining solution for staining of dead nucleated cells CS1-0109-5mL The ViaStain™ PI Staining Solution enables the user to quantitatively distinguish live and dead cells in pure cultures using the Cellometer system. Propidium iodide (PI) is a membrane exclusion nucleic acid dye that only enters cells with compromised membranes (dead cells). When cell counting is performed using both bright-field and fluorescent imaging, cells that fluoresce in red are counted as dead, while the bright-field image provides the total cell count. The Cellometer software utilizes the total cell count and dead cell count to calculate [...]

By Polly Mitchell|2021-03-31T13:39:07+00:00March 30th, 2021|0 Comments

Product Insert: ViaStain AO

Download the Product Insert AO (acridine orange) staining solution for staining of nucleated cells CS1-0108-5mL The ViaStain™ AO Staining Solution enables the user to quantitatively determine total cell count in both pure cultures and primary samples using the Cellometer system. Acridine Orange (AO) is a nucleic acid dye that enters cells and binds to the DNA inside the nucleus. When cell counting is performed using fluorescent imaging, all cells that fluoresce green are counted, while the debris or non-cell particles are excluded, enabling accurate determination of concentration using the Cellometer system. Learn more about Cell Counting / Viability Reagents

By Polly Mitchell|2021-03-31T13:38:10+00:00March 30th, 2021|0 Comments

Product Insert: ViaStain AO/PI

Download the Product Insert AO/PI (acridine orange / propidium iodide) staining solution for live/dead Mammalian nucleated cells CS2-0106-5mLCS2-0106-25mL The ViaStain™ AOPI Staining Solution in PBS enables the user to quantitatively distinguish live and dead nucleated cells from a variety of primary mammalian cell samples, even in the presence of a high background of non-lysed red blood cells, platelets and/or debris using the Cellometer system. This formulation has been optimized to work with whole blood, peripheral blood mononuclear cells (PBMC), bone marrow, splenocytes, thymocytes, lymph nodes and hepatocytes but also works in various other digested tissues and cultured cell lines. The solution contains [...]

By Polly Mitchell|2021-03-31T13:37:06+00:00March 30th, 2021|0 Comments

Assay Protocol: Automated (Auto 2000) vs. Manual Cell Counting

Download the Assay Protocol Recommended Procedure for Comparison of Cell Concentration & Viability using the Cellometer Auto 2000 Automated Cell Viability Counter and a Manual Hemacytometer For researchers performing manual cell counting, a key factor involved in evaluation of an automated cell counter is comparison to their current method. Based on our experience supporting thousands of Cellometer customers in the field, we have designed the following protocol to make comparison of the Cellometer Auto 2000 Cell Viability Counter to manual hemacytometer results as accurate as possible. For the comparison to be valid, Auto 2000 and hemacytometer counts must be done [...]

By Polly Mitchell|2021-03-30T18:57:37+00:00March 30th, 2021|0 Comments

Assay Protocol: Automated (Auto T4) vs. Manual Cell Counting

Download the Assay Protocol Recommended Procedure for Comparison of Cell Concentration & Viability using the Cellometer Auto T4 Automated Cell Counter and a Manual Hemacytometer For researchers performing manual cell counting, a key factor involved in evaluation of an automated cell counter is comparison to their current method. Based on our experience supporting thousands of Cellometer Auto T4 customers in the field, we have designed the following protocol to make comparison of the Cellometer Auto T4 Cell Counter to manual hemacytometer results as accurate as possible. For the comparison to be valid, Auto T4 and hemacytometer counts must be done [...]

By Polly Mitchell|2021-03-30T18:54:39+00:00March 30th, 2021|0 Comments

Assay Protocol: Yeast Dilution Buffer

Download the Assay Protocol Yeast Dilution Buffer The ViaStain™ Yeast Dilution Buffer in combination with the ViaStain™ Yeast Live / Dead Cell staining solution enables the user to quantitatively distinguish live and dead yeast in pure cultures and in cultures containing debris such as corn mash using the Cellometer system. The Dilution Buffer is used to dilute the yeast sample in order to promote and optimize live / dead fluorescent staining of the yeast cells.

By Polly Mitchell|2021-03-30T18:43:20+00:00March 30th, 2021|0 Comments

Assay Protocol: Yeast Viability

Download the Assay Protocol AO/PI Yeast Viability Kit containing dilution buffer and fluorescent dye mixture for staining of live and dead Yeast cells The ViaStain™ Yeast Live / Dead Cell Counting Kit enables the user to quantitatively distinguish live and dead yeast in pure cultures and in cultures containing debris such as corn mash using the Cellometer system. The kit contains a solution of the green-fluorescent nucleic acid stain, acridine orange, and the red-fluorescent nucleic acid stain, propidium iodide, and a dilution buffer. Propidium iodide is a membrane exclusion dye that only enters cells with compromised membranes while acridine orange [...]

By Polly Mitchell|2021-03-30T18:36:40+00:00March 30th, 2021|0 Comments

Assay Protocol: Yeast Vitality

Download the Assay Protocol The ViaStain™ CFDA-AM Yeast Vitality Stain enables breweries to detect metabolically active Lager and Ale yeast at different fermentation stages The ViaStain™ CFDA-AM Yeast Vitality Stain enables the user to quantitatively distinguish metabolically active Lager and Ale yeast in pure cultures and in cultures containing debris such as beer slurries using the Cellometer system. The stain contains a solution of a cell-permeant esterase substrate, CFDA-AM (5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester), that fluoresces green when hydrolyzed by enzymatically active Lager and Ale yeast. The percent of yeasts that are actively fermenting during production can be determined and used [...]

By Polly Mitchell|2021-03-30T18:31:41+00:00March 30th, 2021|0 Comments

Assay Protocol: Mammalian Nucleated Cells

Download the Assay Protocol AO/PI (acridine orange / propidium iodide) Staining Solution for live/dead Mammalian nucleated cells The ViaStain™ AO/PI Staining Solution in PBS enables the user to quantitatively distinguish live and dead nucleated cells from a variety of primary mammalian cell samples, even in the presence of a high background of non-lysed red blood cells, platelets and/or debris using the Cellometer system. This formulation has been optimized to work with whole blood, peripheral blood mononuclear cells (PBMC), bone marrow, splenocytes, thymocytes, lymph nodes and hepatocytes but also works in various other digested tissues and cultured cell lines. The solution [...]

By Polly Mitchell|2021-03-30T18:26:31+00:00March 30th, 2021|0 Comments

Assay Protocol: Dead Nucleated Cells

Download the Assay Protocol PI (propidium iodide) Staining Solution for staining of dead nucleated cells The ViaStain™ PI Staining Solution enables the user to quantitatively distinguish live and dead cells in pure cultures using the Cellometer system. Propidium iodide (PI) is a membrane exclusion nucleic acid dye that only enters cells with compromised membranes (dead cells). When cell counting is performed using both bright-field and fluorescent imaging, cells that fluoresce in red are counted as dead, while the bright-field image provides the total cell count. The Cellometer software utilizes the total cell count and dead cell count to calculate percent [...]

By Polly Mitchell|2021-03-30T18:21:01+00:00March 30th, 2021|0 Comments

About Polly Mitchell