Accurately Count PBMC and Measure Viability in Presence of Residual RBC

It’s White Paper Wednesday! This month’s featured white paper: Accurately Count PBMC and Measure Viability in Presence of Residual RBC In this work, we have developed an image cytometry method for detecting and monitoring the cell expansion and differentiation of articular chondrocytes in primary culture. First, the feasibility of utilizing image cytometry for detection of fluorescent is shown by comparing measured fluorescent positive cell populations to flow cytometry. Next, articular chondrocyte cultures were established in multi-well plates from either single or Cyan/eGFP double reporter mouse lines and grown for 20 days to test the utility of the fluorescence-based image cytometry system. [...]

Impact of RBC contamination in clinical samples

Bone marrow, cord blood, whole blood, and peripheral blood are routinely processed in many different laboratories. Whether for cryopreservation or for downstream isolation of specific nucleated cells, (such as stem cells, B-cells, or T-cells) accurately measuring cell concentration and viability is paramount to the overall success of the project. Many blood-based samples (whole blood, peripheral blood, bone marrow, PBMC, cord blood, etc…) may contain residual red blood cells even after RBC lysis. (See figure below) When samples are enumerated using manual counting, the presence of residual RBCs can lead to inaccurate cell concentration and viability readings. Nucleic acid dyes, acridine [...]

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