Video: Cell Counting Method Development for CAR-T Cell Therapy
Topics covered in this webinar include cell counting is an important step for CAR T cell therapy that is often overlooked, trypan blue is the traditional method for cell counting, but has 2 inherent issues (RBC contaminations can over estimate cell count and viability, trypan blue affecting dead cell morphology under estimating dead cells), using nuclear FL stains AOPI on Cellometer can eliminate the issues caused by trypan blue, there are guidelines to qualifying cell counting method, which we have adopted for our technologies.