Practical Application of ISO Cell Counting Standards to Improve the Quality of Cell Counting Measurements
Biological samples range in complexities and require various considerations, formulations, and bioprocessing steps depending on intended utilization. As there are no ground truth reference materials for live cells, determining the accuracy of cell counting becomes challenging. Therefore, to increase the confidence of cell counting results, following the ISO cell counting standards is necessary to ensure the appropriate cell counting methods are selected and evaluated.
In this webinar, we will discuss six key factors impacting the selection of a fit-for-purpose cell counting method and the quality of measurements, as described in the ISO Cell Counting Standard ISO 20391-1:2018 Biotechnology – Cell counting – Part 1: General guidance on cell counting methods.
- Determine the intended use of the intended cell counting result
- Investigate to understand cell sample composition
- Methodologies of cell population identification
- Cell counting system considerations
- Cell counting process optimization
- Consistent and robust operator training
As a member of the US Technical Advisory Group, led by the National Institute of Standards and Technology (NIST) in collaboration with FDA, we have actively participated and contributed to the development of the ISO Cell Counting Standards 20391-1:2018 Part I and ISO 20391-2:2019 Part II: Experimental design and statistical analysis to quantify counting method performance.
Leo Chan, PhD
Technology Research and Development Manager | Nexcelom Bioscience
Leo Chan currently serves as the Technology Research and Development Manager at Nexcelom Bioscience LLC in Lawrence Massachusetts. For over ten years his research has involved the development of research, instrumentation, and applications for the Cellometer, Cellaca, and Celigo high-throughput image cytometry systems. His primary focus is on cell counting and education for image-based technologies for the detection and analysis of cell-based assays in the field of immuno-oncology, cell/gene therapy, regenerative medicine, protein science, and microbiology. He is a member of the American Association for Cancer Research (AACR) and The American Association of Immunologists (AAI).
Jean Qiu, PhD
Chief Technology Officer, Founder | Nexcelom Bioscience
Dr. Qiu earned her Ph.D. in Electrical Engineering from Purdue University in 1990 and conducted her postdoctoral research at 3M’s Corporate Research Lab. She is a pioneer of accurate cell counting methodologies, an inventor with 34 patents, and an accomplished researcher with over 45 publications in peer-reviewed academic journals. She is a member of the US Technical Advisory Group to the International Organization for Standardization (ISO) Technical Committee where she continuously contributes to the development of new cell counting and viability standards for a variety of challenging cell types in complex sample conditions.