Do I need any special buffer or reagent for cell counting?
No special buffer is required. Cells suspended in growth media or PBS are routinely counted. Trypan blue or AO/PI (CS2-0106) is used for viability assays. For yeast counting and viability, the yeast cells need to be first diluted in a Yeast Dilution Buffer from Nexcelom prior to staining with Yeast AO/PI (CSK-0102).