How can you quickly set up the exposure time of different channels in the scan tab?
For the Celigo Image Cytometer: Setup the focus for the channel that has content in every well first, e.g. brightfield or hoechst stained cells. Then switch to another channel setup the focus offset, then use the pixels in the objects to determine if the exposure needs to be increased or decreased. The pixel range is 0 (black) to 255 (white). Ideally, object pixels should be between 50-90% of that range (135-250). Repeat this for each of the channels. Save this as an experiment/projcet for settings to be automatically applied in the next scan.