I have a Cellometer Vision with a VB-535-402 and VB-595-502 filter set and I am using AO/PI. What formula should I use for viability?
The correct formula is: F1/F2 x 100%. The reason for using this formula is because the Green channel (VB-535-402) will show live cells and red channel (VB-595-502) channel will show all cells. Thus the formula: F1 (Live)/F2 (Total) x 100%. For our other dual fluorescent instruments (Cellometer Auto 2000, Cellometer X2, Cellometer K2) that have fixed VB-535-402 and VB-660-XXX, the formula for viability when using AO/PI is F1/(F1+F2) x 100%