///Cellometer Auto 1000 Automated Cell Counter
Cellometer Auto 1000 Automated Cell Counter 2018-06-26T16:35:38+00:00

Cellometer Auto 1000 Bright Field Cell Counter

Touchscreen automated cell counter for trypan blue viability

Why Auto 1000?

  1. Trypan Blue Viability in < 10 Seconds!
  2. Analysis of Clumpy & Irregular-Shaped Cells
  3. Cell Size Analysis & Size-based Counting
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The Cellometer Auto 1000 is fast and was easy to purchase with an on-time delivery for my experiments! Good quality cell counter. Our results are very reproducible and reliable!

Roshan Kumari, UTHSC

The Cellometer Auto 1000 is the best automatic cell counter because it always counts the cells consistently and accurately!

Margarete Hafley, UT MD Anderson Cancer Center

Our lab purchased a Cellometer Auto 1000 a year ago, and it has been a great pleasure ever since. Cell counting that could take a half day to do using the hemocytometer takes only half an hour.

Zhe Zhang, MIT

Our customers include:

 

Introduction to the Cellometer Auto 1000 Bright Field Cell Counter

Trypan Blue Viability in < 10 Seconds!

Cellometer Auto 1000 Trypan Blue Viability ResultsThe Auto 1000 utilizes bright field imaging to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Cell count, concentration, diameter, and % viability are automatically calculated and reported in < 10 seconds.

Simple, User-friendly Procedure

Pipette 20 µl

load sample use with Cellometer Auto 1000

Insert Slide

Insert Slide into the Cellometer Auto 1000

Select Assay, Count

Select Assay, Count - using the Cellometer Auto 1000

View Images & Results

View Images & Results with the Cellometer Auto 1000

Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cells identified with Cellometer Auto 1000

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the counted image of A549 cancer cells shown at left.

Irregular-shaped Cells identified with Cellometer Auto 1000

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at left.

Cell Size Analysis & Size-based Counting

Cell Size Analysis & Size-based Counting wih the Cellometer Auto 1000 graphThe Cellometer Auto 1000 automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.

Stand-Alone Instrument with Large Touch Screen

User-friendly touch screen on Cellometer Auto 1000

The Cellometer Auto 1000 is a convenient all-in-one system. The built-in computer can be lined to a network via USB cable or a USB drive can be used to automatically save counting data.

The large 10″ Auto 1000 touch-screen enables simple operation and user-friendly visualization of cell morphology and counted cells. Easily select saved assays with optimized counting parameters for specific cell types.

Counted Cell Images for Data Verification

Counted Cell Images for Data Verification using Cellometer Auto 1000No two cells are the same. With the Auto 1000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells can be viewed for further verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Cells within small clusters are being counted individually
  • Debris is being excluded

More than 20x Faster than Manual Counting

Hemacytometer under microscope - Cellometer Auto 1000 is more than 20x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 1000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in < 10 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Reduce Inter-Operator Variability
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer Auto 1000 cell counting chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Auto-Save Data and Cell Images

Print

Print directly from the instrument screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the Auto-Print setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network or USB drive. Activate the Auto-Save feature for data, raw images, and/or counted images. They will automatically be saved to a specified network location or USB drive following analysis of each sample.

Dedicated On-line and On-site Applications Support

Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Auto 1000 Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Applications for Cellometer Auto 1000 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Auto 1000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.03E+06 1.05E+06
STDEV 6.60E+04 7.76E+04
%CV 6.4% 7.4%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.07E+06 1.15E+06
STDEV 5.90E+04 6.35E+04
%CV 5.5% 5.5%

The Cellometer Auto 1000 is the best cell counter I have ever used! It does not require cell staining, easily and rapidly offers the cell concentrations and can also provide cell diameters. This machine is user-friendly and does not need complex steps. Also, it can store the data for many measured times. I love this cell counter and would recommend to friends in other labs!

Pengchao Zhang, Houston Methodist Research Institute

The Cellometer Auto 1000 is a great machine for cell counting and cell size analysis. It is very easy to save and transfer data with a USB drive

Joseph Liu, UTHSCSA Biotechnology

Our Cellometer Auto 1000 has greatly sped up our work! Cell counts load so much faster, and our favorite part was the set up. Nexcelom’s representatives came out multiple times to make sure all of our cell types could be counted efficiently and effectively. They were a pleasure to work with!

Talia Just, Northwestern University

The Cellometer Auto 1000 is great! Everyone in my lab loves to use it! We count everything from human cells to insect cells.

With the Cellometer Auto 1000, I can use the full amount of my precious samples to do more than just two assays! And with knowing the accurate cell count, I can give more of a precise count of levels of my target cell type.

The Cellometer Auto 1000 is fast and was easy to purchase with an on-time delivery for my experiments! Good quality cell counter. Our results are very reproducible and reliable! – Roshan Kumari, UTHSC

The Cellometer Auto 1000 is the best cell counter I have ever used! It does not require cell staining, easily and rapidly offers the cell concentrations and can also provide cell diameters. This machine is user-friendly and does not need complex steps. Also, it can store the data for many measured times. I love this cell counter and would recommend to friends in other labs! – Pengchao Zhang, Houston Methodist Research Institute

The Cellometer Auto 1000 is an excellent cell counter with an integrated touch screen computer. Works great, east to use, and very convenient to transfer data!

The Cellometer Auto 1000 is great! We use it for counting all types of cells and to also look at it’s quick phenotype!

We had a demo with the Cellometer Auto 1000, and it worked great. Overall, it is a lot faster then our other reader and has a larger range of acceptable concentrations. It is robust enough to keep up with our labs needs.

Our Cellometer Auto 1000 has greatly sped up our work! Cell counts load so much faster, and our favorite part was the set up. Nexcelom’s representatives came out multiple times to make sure all of our cell types could be counted efficiently and effectively. They were a pleasure to work with! – Talia Just, Northwestern University

The Cellometer Auto 1000 is the best automatic cell counter because it always counts the cells consistently and accurately! -Margarete Hafley, UT MD Anderson Cancer Center

The Cellometer Auto 1000 has made my ability to count cells much quicker with more accuracy and consistency.

Our lab is currently using the Cellometer Auto 1000. It is very convenient, quick and easy to count cells on live preview with a touchscreen.

The Cellometer Auto 1000 is a great machine for cell counting and cell size analysis. It is very easy to save and transfer data with a USB drive. -Joseph Liu, UTHSCSA Biotechnology

Our lab purchased a Cellometer Auto 1000 a year ago, and it has been a great pleasure ever since. Cell counting that could take a half day to do using the hemocytometer takes only half an hour. Besides, viability test and size measurement also becomes very simple and easy. This automated cell counter really increased the efficiency and accuracy of our cell counting. – Zhe Zhang, MIT

We’re using Cellometer Auto 1000 for regular cell counting. Now it becomes so easy to do this. Just with seconds, it’s done. Great instrument!