Cellometer Auto 1000 Bright Field Cell Counter

Touchscreen automated cell counter for trypan blue viability

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Introduction to the Cellometer Auto 1000 Bright Field Cell Counter

Trypan Blue Viability in Less than 10 Seconds!

The Auto 1000 utilizes bright field imaging to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Cell count, concentration, diameter, and % viability are automatically calculated and reported in < 10 seconds.

  • Improve counting of live/dead cells, individual cells within clusters, and irregular-shaped cells with Proprietary Cellometer software
  • Convenient stand-alone system with a large, user-friendly touch screen
  • View, print, and save counted cell images, data reports, and cell size histograms:
    Connect to a network or use a USB drive

Cellometer Auto 1000 Trypan Blue Viability Results

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Brightfield cell counting is recommended only for cell lines or purified/homogenous primary samples. Fluorescence cell counting is recommended for PBMCs, mouse splenocytes, hepatocytes, and other similar primary nucleated cell samples. To see our excellent line of fluorescent cell counters, please visit our fluorescent cell counters page.

Simple, User-friendly Procedure

Pipette 20 µl
load sample use with Cellometer Auto 1000

Insert slide
Insert Slide into the Cellometer Auto 1000

Select assay
Select Assay, Count - using the Cellometer Auto 1000

View images & data
View Images & Results with the Cellometer Auto 1000

Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cells identified with Cellometer Auto 1000

Irregular-shaped Cells identified with Cellometer Auto 1000

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the counted image of A549 cancer cells shown at left.

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at left.

Cell Size Analysis & Size-based Counting

The Cellometer Auto 1000 automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.

Cell Size Analysis & Size-based Counting wih the Cellometer Auto 1000 graph

Stand-Alone Instrument with Large Touch Screen

User-friendly touch screen on Cellometer Auto 1000

The Cellometer Auto 1000 is a convenient all-in-one system. The built-in computer can be lined to a network via USB cable or a USB drive can be used to automatically save counting data.

The large 10″ Auto 1000 touch-screen enables simple operation and user-friendly visualization of cell morphology and counted cells. Easily select saved assays with optimized counting parameters for specific cell types.

Counted Cell Images for Data Verification

No two cells are the same. With the Auto 1000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells can be viewed for further verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Cells within small clusters are being counted individually
  • Debris is being excluded

Counted Cell Images for Data Verification using Cellometer Auto 1000

More than 20x Faster than Manual Counting

Hemacytometer under microscope - Cellometer Auto 1000 is more than 20x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 1000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in < 10 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Reduce Inter-Operator Variability
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer Auto 1000 cell counting chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Auto-Save Data and Cell Images

Print

Print directly from the instrument screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the Auto-Print setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network or USB drive. Activate the Auto-Save feature for data, raw images, and/or counted images. They will automatically be saved to a specified network location or USB drive following analysis of each sample.

Dedicated On-line and On-site Applications Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Auto 1000 Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Support

Applications for Cellometer Auto 1000 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Auto 1000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.03E+06 1.05E+06
STDEV 6.60E+04 7.76E+04
%CV 6.4% 7.4%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.07E+06 1.15E+06
STDEV 5.90E+04 6.35E+04
%CV 5.5% 5.5%

Customer Testimonials

Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.


Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.


Cynthia Noguera

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.

Cynthia Noguera

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.


BennuBio Inc.

Aurora Fabry-Wood

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.

BennuBio Inc.

Aurora Fabry-Wood

Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.


Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.

More of what our customers have to say about Nexcelom products –

The customer service is good too

We use [Cellometer] to count PBMC and tumor cells, Auto T4 is easy and good to use for measuring. The customer service is good too, willing to come and help set the optimization of the instrument. We recommend not only these machines, but this company also.


Great

It is easy to operate. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. It also provides cell sizes and distribution that are very informative to help you know your cells better. This instrument is already trained for many commonly used cells in the lab. Great.


A vast improvement

We are in the process of validating the instrument [Cellometer Auto 2000] prior to going live. However, the instrument is very easy to use and is a vast improvement over our previous manual counting method.


Donald Hudspeth

Cord For Life, Inc.

Very satisfied

Using the Auto 2000 has saved us a lot of time that would have been wasted on using traditional counting methods. The counter has proven to be very reliable when doing counts in duplicates. Overall, we are very satisfied with this equipment.


Consistent and dependable

We use Cellometer K2 for cell counting. This is so convenient and easy to use. Each slide can be used for two different cell lines and the results show the total number of cells, the number of live cells and dead cells. So we can use this for a lot of experiments which are based on cell numbers and make our results more consistent and dependable.


Shan Lu

Laboratory of Cellular & Molecular Radiation Oncology, MGH

Worth the investment

This instrument [Cellometer K2] is definitely worth the investment. It has a plethora of uses and now serves as the go-to instrument for not only counting cells but for measuring the titer of baculovirus-infected insect cells. Additionally, the customer service representatives are very helpful.


Very convenient

This instrument [Cellometer Auto 2000] is very convenient especially for counting the cell number of multiple samples. However, I find that it is a bit difficult to uncover the cover membrane of the slide.


The Jackson laboratory

Save a lot of time

We’ve been able to save a lot of time in our yeast counting protocol, especially when working with multiple samples. Prepping and counting 5 samples doesn’t take much longer than doing just one and being able to automatically save the data makes record keeping a breeze.


Visualize

The AO/PI live/dead dye used with the Cellometer Auto 2000 is a great way to see and measure the proportion of live and dead cells in suspension. You can visualize if the dead cells are dispersed evenly or if clumping is a factor in cell viability.


Reproducible results

In comparison to the standard method for manual cell counting, Cellometer K2 helps me in counting cells efficiently and quickly with reproducible results. I use it mainly for assessing cell vitality by trypan blue exclusion assay.


Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks, as well as contributing to our research studies. It is very straightforward to use.


Would definitely recommend

Cellometer Auto T4 has made my life easy. Now I don’t have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. Cellometer is very easy to use, fast and accurate. The best thing is that I can optimize the settings for counting different cells on the basis of size and it can even count clumpy cell lines. I would definitely recommend this instrument to everyone.


Shweta Singh

Allowed us to enter a new field of cell culture

The K2 has allowed us to enter a new field of cell culture with best practices already in place. Not having to optimize the use of a cell counter will save us a lot of time.


Zachary Schools

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a daily basis. We mainly use it to count live or dead cells in a population and to maintain viable levels of NK92mi cells in the desired concentration range.


Very easy to use

The Auto T4 is very easy to use and the software is very friendly. I save a lot of time when I count number of cells and more when I am using more than one cell line.


This machine has transformed the way I culture

This machine [Cellometer K2] has transformed the way I culture. No longer do I have to spend hours out of my day to manually could cells. It has changed the way I see cell culture from a menial chore to something I look forward to every day. Cheers NEXCELOM for a job well done!!!!


Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable option for the precision being offered. This instrument is a great addition to any lab!


Brett Haines

Utmost confidence

Using the X2 has revolutionized our ability to monitor our overall yeast health and performance. Allowing us to accurately harvest and re-pitch our yeast strains for successive generations with the utmost confidence.


Hangar 24 Craft Brewing

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.


BennuBio Inc.

Aurora Fabry-Wood

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we used to do to count our cells. We count everything from CHO-k1, PMBCs, and mast cells using this machine. We particularly find this method of counting cells is far superior to any other method we have tried when counting cells from a tissue digest. Would highly recommend this machine.


You can trust your results

I enjoy the ease of use of the Auto 2000 – everyone in the lab uses it and we have consistent results when counting samples. It is also helpful to see which cells are counted as dead and alive, so you can trust your results.


User-friendly

Good Purchase. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly.


Very impressed

After testing this cell counter and comparing it to other counting methods, we have seen that the Cellaca MX produces tighter/accurate counts and saves us time. I was very impressed with the Field Service Representatives from Nexcelom for their willingness to come out to teach and troubleshoot any issues we were having. Big shoutout to Andrea Love and Charles Hernandez.


Kimberly Sandy

Pfizer

Accuracy we require

The Cellaca MX high-throughput cell counter has streamlined the development efforts of our cell therapy product. The instrument combines the accuracy we require for cell viability measurements using the dual live/dead fluorescent dyes with the automation needed for intensive characterization studies. The support team at Nexcelom has been very supportive in getting our instrument up and running, with our product-specific requirements. The time savings for our team with the increased speed and automation will easily justify the cost of the instrument.


Will Heaton

DiscGenics

Customer Publications using Cellometer Auto 1000

Our customers include: