Cellometer Auto 1000 Brightfield Cell Counter

This product is no longer manufactured. Nexcelom recommends the advanced capabilities of the Cellometer Auto T4 as an alternative.

Buy the Cellometer Auto T4 Online

Introduction to the Cellometer Auto 1000 Brightfield Cell Counter

Trypan Blue Viability in Less than 10 Seconds!

The Auto 1000 utilizes bright field imaging to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Cell count, concentration, diameter, and % viability are automatically calculated and reported in < 10 seconds.

  • Improve counting of live/dead cells, individual cells within clusters, and irregular-shaped cells with Proprietary Cellometer software
  • Convenient stand-alone system with a large, user-friendly touch screen
  • View, print, and save counted cell images, data reports, and cell size histograms:
    Connect to a network or use a USB drive

Cellometer Auto 1000 Trypan Blue Viability Results

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Brightfield cell counting is recommended only for cell lines or purified/homogenous primary samples. Fluorescence cell counting is recommended for PBMCs, mouse splenocytes, hepatocytes, and other similar primary nucleated cell samples. To see our excellent line of fluorescent cell counters, please visit our fluorescent cell counters page.

Simple, User-friendly Procedure

Pipette 20 µl
load sample use with Cellometer Auto 1000

Insert slide
Insert Slide into the Cellometer Auto 1000

Select assay
Select Assay, Count - using the Cellometer Auto 1000

View images & data
View Images & Results with the Cellometer Auto 1000

Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cells identified with Cellometer Auto 1000

Irregular-shaped Cells identified with Cellometer Auto 1000

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the counted image of A549 cancer cells shown at left.

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at left.

Cell Size Analysis & Size-based Counting

The Cellometer Auto 1000 automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.

Cell Size Analysis & Size-based Counting wih the Cellometer Auto 1000 graph

Stand-Alone Instrument with Large Touch Screen

User-friendly touch screen on Cellometer Auto 1000

The Cellometer Auto 1000 is a convenient all-in-one system. The built-in computer can be lined to a network via USB cable or a USB drive can be used to automatically save counting data.

The large 10″ Auto 1000 touch-screen enables simple operation and user-friendly visualization of cell morphology and counted cells. Easily select saved assays with optimized counting parameters for specific cell types.

Counted Cell Images for Data Verification

No two cells are the same. With the Auto 1000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells can be viewed for further verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Cells within small clusters are being counted individually
  • Debris is being excluded

Counted Cell Images for Data Verification using Cellometer Auto 1000

More than 20x Faster than Manual Counting

Hemacytometer under microscope - Cellometer Auto 1000 is more than 20x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 1000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in < 10 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Reduce Inter-Operator Variability
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer Auto 1000 cell counting chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Auto-Save Data and Cell Images

Print

Print directly from the instrument screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the Auto-Print setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network or USB drive. Activate the Auto-Save feature for data, raw images, and/or counted images. They will automatically be saved to a specified network location or USB drive following analysis of each sample.

Dedicated On-line and On-site Applications Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Auto 1000 Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Support

Applications for Cellometer Auto 1000 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Auto 1000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.03E+06 1.05E+06
STDEV 6.60E+04 7.76E+04
%CV 6.4% 7.4%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.07E+06 1.15E+06
STDEV 5.90E+04 6.35E+04
%CV 5.5% 5.5%

Customer Testimonials

Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.

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Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.

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High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.

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Cynthia Noguera

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.

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Cynthia Noguera

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.

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BennuBio Inc.

Aurora Fabry-Wood

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.

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BennuBio Inc.

Aurora Fabry-Wood

Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.

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Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.

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More of what our customers have to say about Nexcelom products –

Huge time savings

Huge time savings when counting large numbers of samples, very easy to use.

Jonathan Portman

Affinivax, Inc.

Very supportive staff

Ease of use is probably the most dominating feature [of the Cellometer Auto 2000]. Backed by a very supportive staff, using instruments or services through Nexcelom has been a pleasure.

Edgard Raymond

Very convenient and time-saving

The [Cellometer] Auto T4 we have in the lab is a very convenient and time-saving instrument, especially, when we have a lot of samples to quantify the cell number and cell viability. We like it very much!

Sherleen Adamson

Very satisfied

We have the [Cellometer] Mini and Vision and are very satisfied with both instruments.

Walter Reed Army Institute of Research

We are confident in the accuracy

This instrument is very helpful in our lab – we use it for AO/PI, trypan, and bright field staining for at least 5 different cell types and we are confident in the accuracy of the [Cellometer] Auto 2000.

Extremely useful

The Auto 2000 is extremely useful for our laboratory. We use one a lot of times during our day. Cellometer helps do experiments much faster than before.

Svetlana Gapon

Brigham and Women's Department of Pathology

Simple and intuitive

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Hanh Chi Do

Northwestern University

Fast and reliable

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Array Biopharma, Inc.

Quickly measure yeast viability

I love my Nexcelom X2 Cellometer! It allows me to quickly measure yeast viability in my lab and for my customers! No more tedious counting yeast cells under the microscope via methylene blue.

Sean Sleight

Sleight Beer Lab

Improve yeast health

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Lindsey Kingry

Very quick and efficient

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Speeds up my work significantly

The [Cellometer] Auto T4 is accurate and easy to use. It speeds up my work significantly. I love it!

Ningwu Huang

Riboscience LLC

Reproducible results

[Cellometer] Auto T4 is easy to use and allow us to get a reproducible result. We are satisfied with the function. It gets better if you have the autofocus function.

AWESOME!

So much faster than traditional cell counting!! I mostly do FBS tests & this requires counting many, many wells!!! I love that now I can do an hour and a half of painstaking cell counting in about 10 minutes!!! AWESOME!

University of Virginia

This machine works great!

This machine [Cellometer Auto 2000] works great! So much easier and more accurate than counting manually.

See my cell suspension prior to counting

I really love that I can use the Cellometer Auto 2000 to see my cell suspension prior to counting. If my cells look too dense and clumpy, I can further dilute my cell suspension and test another sample to see that they are suspended in sufficient volume to get accurate counts.

Everyone in the lab prefers the Cellometer

We have multiple counting instruments (flow cytometer, trypan blue, Scepter, Nucleocounter) but everyone in the lab prefers the Cellometer [Auto 2000] due to its reliability and ease of use.

Convenience and speed

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Stephanie Sieja

Unique features

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Alexei Kisselev

Auburn University

Fancy and compact

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Reliable machine

The instrument [Cellometer K2] is being used in our lab for counting different types of immune cells, mostly neutrophils (but enucleated cells as well). It has been a reliable machine, no problems in general.

The University of Alabama at Birmingham

Shortens the time of our protocol

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Marcus Davis

University of Alabama at Birmingham

Very amenable to customization

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Lindsay Sulzer

TeVido BioDevices

Ease of use

Ease of use is probably the most dominating feature. Backed by a very supportive staff, using instruments or services through Nexcelom has been a pleasure.

John Pleau

Customer Publications using Cellometer Auto 1000

Our customers include: