Cellometer Auto 1000 Bright Field Cell Counter

Touchscreen automated cell counter for trypan blue viability

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Introduction to the Cellometer Auto 1000 Bright Field Cell Counter

Trypan Blue Viability in Less than 10 Seconds!

The Auto 1000 utilizes bright field imaging to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Cell count, concentration, diameter, and % viability are automatically calculated and reported in < 10 seconds.

  • Improve counting of live/dead cells, individual cells within clusters, and irregular-shaped cells with Proprietary Cellometer software
  • Convenient stand-alone system with a large, user-friendly touch screen
  • View, print, and save counted cell images, data reports, and cell size histograms:
    Connect to a network or use a USB drive

Cellometer Auto 1000 Trypan Blue Viability Results

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Brightfield cell counting is recommended only for cell lines or purified/homogenous primary samples. Fluorescence cell counting is recommended for PBMCs, mouse splenocytes, hepatocytes, and other similar primary nucleated cell samples. To see our excellent line of fluorescent cell counters, please visit our fluorescent cell counters page.

Simple, User-friendly Procedure

Pipette 20 µl
load sample use with Cellometer Auto 1000

Insert slide
Insert Slide into the Cellometer Auto 1000

Select assay
Select Assay, Count - using the Cellometer Auto 1000

View images & data
View Images & Results with the Cellometer Auto 1000

Analyze Individual Cells in Clusters and Irregular-Shaped Cells

Clumpy Cells identified with Cellometer Auto 1000

Irregular-shaped Cells identified with Cellometer Auto 1000

Clumpy Cells

The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the counted image of A549 cancer cells shown at left.

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at left.

Cell Size Analysis & Size-based Counting

The Cellometer Auto 1000 automatically generates a cell size histogram based on cell diameter.

The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.

Cell Size Analysis & Size-based Counting wih the Cellometer Auto 1000 graph

Stand-Alone Instrument with Large Touch Screen

User-friendly touch screen on Cellometer Auto 1000

The Cellometer Auto 1000 is a convenient all-in-one system. The built-in computer can be lined to a network via USB cable or a USB drive can be used to automatically save counting data.

The large 10″ Auto 1000 touch-screen enables simple operation and user-friendly visualization of cell morphology and counted cells. Easily select saved assays with optimized counting parameters for specific cell types.

Counted Cell Images for Data Verification

No two cells are the same. With the Auto 1000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells can be viewed for further verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Cells within small clusters are being counted individually
  • Debris is being excluded

Counted Cell Images for Data Verification using Cellometer Auto 1000

More than 20x Faster than Manual Counting

Hemacytometer under microscope - Cellometer Auto 1000 is more than 20x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 1000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in < 10 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Reduce Inter-Operator Variability
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments

Counting Chambers – No Washing or Contamination

Cellometer Auto 1000 cell counting chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume

Auto-Save Data and Cell Images

Print

Print directly from the instrument screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the Auto-Print setting to automatically generate a report for each cell count.

Save

Easily Save data and images to a network or USB drive. Activate the Auto-Save feature for data, raw images, and/or counted images. They will automatically be saved to a specified network location or USB drive following analysis of each sample.

Dedicated On-line and On-site Applications Support

Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Cellometer Auto 1000 Cell Counter

All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or support@nexcelom.com

Support

Applications for Cellometer Auto 1000 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Performance of the Cellometer Auto 1000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.03E+06 1.05E+06
STDEV 6.60E+04 7.76E+04
%CV 6.4% 7.4%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 1000
Average 1.07E+06 1.15E+06
STDEV 5.90E+04 6.35E+04
%CV 5.5% 5.5%

Customer Testimonials

Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.


Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.


Cynthia Noguera

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.

Cynthia Noguera

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.


BennuBio Inc.

Aurora Fabry-Wood

I was using a hemocytometer…

I was using a hemocytometer…the [Cellometer] Auto 1000 increased both my productivity and the quality of my data! Additionally, I really appreciate having a digital copy of both the counts and the images.

BennuBio Inc.

Aurora Fabry-Wood

Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.


Saves us valuable time and effort

The Cellometer Auto 1000 works quickly and efficiently, saving us valuable time and effort.

More of what our customers have to say about Nexcelom products –

Huge time savings

Huge time savings when counting large numbers of samples, very easy to use.


Jonathan Portman

Affinivax, Inc.

The Cellaca is great!

The Cellaca is great! We have the 5 color one and use it with the standard assays (mainly AO/PI), along with creating some of our own for specialized needs. We run so many samples each day/week and it’s a total time saver. I will never go back to counting individual samples with trypan blue again….

Alaina Puleo

10X Genomics

Makes cell counting easy!

The Cellaca makes cell counting easy!


Evgeny Kiner

Immunai Inc.

Confidence in our data

The Cellaca is a very useful instrument in our lab. It is very easy to use and we are able to count our samples quickly and accurately so that we can move onto our next steps. This saves on time and labor and gives us more confidence in our data. When we have issues arise, the technical help we have…

Katherine Henderson

Allen Institute for Immunology

Accuracy we require

The Cellaca MX high-throughput cell counter has streamlined the development efforts of our cell therapy product. The instrument combines the accuracy we require for cell viability measurements using the dual live/dead fluorescent dyes with the automation needed for intensive characterization studies…

Will Heaton

DiscGenics

Very impressed

After testing this cell counter and comparing it to other counting methods, we have seen that the Cellaca MX produces tighter/accurate counts and saves us time. I was very impressed with the Field Service Representatives from Nexcelom for their willingness to come out to teach and troubleshoot any i…

Kimberly Sandy

Pfizer

Best cell counter on the market

The ease of use and fast, high-throughput capabilities definitely make the Cellaca MX the best cell counter on the market, even over the Beckman Vi-Cell which I’ve used for the past decade. Also, the follow-up with their FAS has been great, making sure that the product still works well with ou…

Jasmine Ta

Eli Lilly

So amazing!

So amazing! No instrument fluidics are to be maintained, it analyses cell lines or primary cells like T cells, PBMCs, and tumor digest, incorporate robotic with API compatible instrument. It [Cellaca MX] is fast enough to count 24 samples in less than 2.5 minutes. There is value for money in general…

Isabella Akwi

Ernest Cook Mengo

Superfast

Superfast counting, easy to handle, accurate results [with Cellaca MX].


Edward Yeh

Level Biotech

10x Faster than Manual Counting

Easy to use, the effectiveness is high enough. Results are reproducible that is important for experiments. This instrument [Cellaca MX] greatly increases the consistency of our experiments. And 10x faster than manual counting, no need for washing or contamination, so it will be excellent for decreas…

Ayman Al-Labban

Qualipharm

Accurate

[Cellaca MX] Super easy to use and fairly high throughput. Great value and accurate cell counts!!!


Tanja Smith

Laboratory Manager

Amazing

Amazing cell counting in a few minutes, many samples collected at the same time [using Cellaca MX].


Dipica Haribhai

AbbVie

Solid, reliable

We were rapidly able to use the Cellaca MX for pipeline PBMC immunology assays integrating both flow cytometry and single-cell genomics platforms. The nuclear staining was useful to exclude RBCs from the cell count. The ability to run a batch of 24 samples in a single plate has been integrated into …

Julian Reading

Allen Institute for Immunology

Wonderful tool

The Celigo has increased our research output and has dramatically sped up our discovery process because of its ease and simplicity of use. It is a wonderful tool for our institution to have.


Franciscan University

Daniel Kuebler

Accuracy

The Celigo has allowed us to implement a Calcein AM cell killing test that uses less transgenic T cells than our previous assays. These cells are precious so using less cells per test means we can implement more experimental conditions than ever before. The accuracy of the cell counting capability h…

Robert Chain

Unmatched & irreplaceable

The excellent cell segmentation abilities of the Celigo are unmatched. Our trust in the Celigo has lead it to be an irreplaceable part of our workflow, and has saved us a huge amount of time.


Tech support was great

I am new to cell culture but using [Cellometer] Auto 1000 was very easy and intuitive. When I did have some issues with saving data, the tech support was great.


A welcome addition

The Nexcelom [Cellometer] X2 unit we received is a welcome addition to our microbiology lab. It was very easy to set up, and a tech came out and walked us through proper usage, and set up assays for our house yeast strains. We are very happy to not have to rely on a hemocytometer and time-consuming …

Hop Valley Brewing Co.

Zachary Foster

High quality

Great product, price, and quality. We have used this product for a long time in our lab and we have no complaints about it. The product shows high quality.


Cynthia Noguera

Highly recommended from a colleague

The Auto T4 Cellometer is easy to set up and intuitive to use. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. This counter came highly recommended from a colleague and so we had to get one for ourselves. It has made counting cells far m…

Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a lot of valuable time that we now can dedicate to other work. We love the Auto 2000!


UCLA

A time-saving machine

Cellometer [T4] has greatly reduced the time it takes to count the cells. There is a large library of settings for different type of cells. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine.


User-friendly

Good Purchase. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly.


You can trust your results

I enjoy the ease of use of the Auto 2000 – everyone in the lab uses it and we have consistent results when counting samples. It is also helpful to see which cells are counted as dead and alive, so you can trust your results.


Customer Publications using Cellometer Auto 1000

Our customers include: