Cellometer Auto 1000 Bright Field Cell Counter
Touchscreen automated cell counter for trypan blue viability
Introduction to the Cellometer Auto 1000 Bright Field Cell Counter
Trypan Blue Viability in Less than 10 Seconds!
The Auto 1000 utilizes bright field imaging to quickly and accurately identify and count individual live cells and dead cells stained with Trypan Blue. Cell count, concentration, diameter, and % viability are automatically calculated and reported in < 10 seconds.
Simple, User-friendly Procedure
Pipette 20 µl
Select Assay, Count
View Images & Results
Analyze Individual Cells in Clusters and Irregular-Shaped Cells
The Cellometer pattern-recognition software identifies and counts individual cells within cell clusters for the most accurate cell counts. One example is the counted image of A549 cancer cells shown at left.
The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and skin cancer cells like the SK-MEL-2 cells shown at left.
Cell Size Analysis & Size-based Counting
The Cellometer Auto 1000 automatically generates a cell size histogram based on cell diameter.
The minimum and maximum cell diameter settings can be optimized to exclude debris or very large cells or to count specific cells in a sample.
Stand-Alone Instrument with Large Touch Screen
The Cellometer Auto 1000 is a convenient all-in-one system. The built-in computer can be lined to a network via USB cable or a USB drive can be used to automatically save counting data.
The large 10″ Auto 1000 touch-screen enables simple operation and user-friendly visualization of cell morphology and counted cells. Easily select saved assays with optimized counting parameters for specific cell types.
Counted Cell Images for Data Verification
No two cells are the same. With the Auto 1000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.
Counted cells can be viewed for further verification that cells in the sample are being imaged and analyzed properly.
Users can confirm that:
- Cells are counted correctly, based on size and shape
- Cells within small clusters are being counted individually
- Debris is being excluded
More than 20x Faster than Manual Counting
Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 1000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in < 10 seconds.
Improve Data Accuracy & Consistency
- Eliminate Wash Steps
- Eliminate Judgment Errors
- Reduce Inter-Operator Variability
- Eliminate Recording & Calculation Errors
- Reduce Counting Time … Run More Experiments
Counting Chambers – No Washing or Contamination
Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber.
The disposable Cellometer Cell Counting Chambers offer several key advantages:
- Time savings – no washing
- No risk of cross-contamination
- Reduced biohazard risk to users
- Controlled sample volume
Auto-Save Data and Cell Images
Print directly from the instrument screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Select the Auto-Print setting to automatically generate a report for each cell count.
Easily Save data and images to a network or USB drive. Activate the Auto-Save feature for data, raw images, and/or counted images. They will automatically be saved to a specified network location or USB drive following analysis of each sample.
Dedicated On-line and On-site Applications Support
Experienced Nexcelom Technical Support Specialists are available from 9 am to 5 pm EST for phone and online support and can assist with:
- Creation of new cell types
- Optimization of counting parameters
- Training of new users
- Installation of a new Cellometer Auto 1000 Cell Counter
All Cellometer users can submit online training requests at www.nexcelom.com/training or contact Nexcelom Technical Support at 978-327-5340 or firstname.lastname@example.org
Applications for Cellometer Auto 1000 Bright Field Cell Counter
Use Cellometer to perform cell-based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »
When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »
Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »
Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »
Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »
Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »
Performance of the Cellometer Auto 1000 Cell Viability Counter
Consistency and Accuracy Comparison to Hemacytometer
|N = 20||Hemacytometer||Cellometer Auto 1000|
5 µm beads
|N = 20||Hemacytometer||Cellometer Auto 1000|
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