“Biological variation” is an easy explanation to reach for when repeated measurements don’t match up very well. It is vague enough to excuse inconsistency in nearly any biological experiment, and in many cases, it is difficult to disprove.
Nexcelom Bioscience provides additional details on how your laboratory can maintain guidelines, meet standards, and ensure proper method selection of counting cells based on your experimentation.
Is your trypan blue rupturing dead or dying immune cells leading to an over-estimation of cell viability?
It is important to select the most accurate cell counting method that is fit-for-purpose where cell number and viability significantly affect treatment efficacy.
The use of modern automation has largely eliminated many of the hemocytometer's sources of error, increasing the accuracy and efficiency of cell counting today.
Nexcelom founder and CTO, Dr. Jean Qiu, describes 3 major reasons why we partner with global and local research organizations to streamline CAR-T workflow.
Q & A regarding the crucial need for accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.