Repurposing immune cells to develop new therapies

For the development of new drugs and the advancement of new cell therapies, pharmaceutical companies have recurred to cytotoxicity assays as a quick way to assess the viability of cell lines in response to an external stimulus.

Establishing a High-throughput Screening Platform for NK mediated Cytotoxicity using Image Cytometry

Researchers highlight the need for large-scale screening of cytotoxic cells and their ability to kill target cell lines in both a 2D and 3D fashion.

Direct CAR NK Therapeutic Targeting for High-Risk Neuroblastoma

Detection of novel CD276-CAR NK-92 mediated cytotoxicity of neuroblastoma tumor spheroids using plate-based image cytometry

Improving the Gene Therapy Development Process using Image Cytometry

Many factors and considerations must be optimized to develop the appropriate cell-based assays for gene therapy development.

Webinar on Demand: Immuno-Oncology Studies

Syngeneic tumor models and human immune cell-engrafted mice are two of the popular preclinical tools in immuno-oncology studies.

Webinar On-Demand: Cell Therapy Development for Solid Tumors

In this webinar, we highlight a variety of assays to support the development of cell therapies for solid tumors.

Nexcelom’s CTO on the Future of CAR T Workflow

Nexcelom founder and CTO, Dr. Jean Qiu, describes 3 major reasons why we partner with global and local research organizations to streamline CAR-T workflow.

Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay

Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.

Poster: High-throughput immune complex analysis method for CAR T cell-mediated cytotoxicity using the Celigo Image Cytometer

Download our Poster Abstract: Cancer immunotherapy has been gaining momentum in the field of cancer research. Specifically, Chimeric Antigen Receptor (CAR) T cell technology have introduced new methods to combat cancer. Direct cell-mediated cytotoxicity assays are required to assess the killing capability of the engineered CAR T cells. Traditionally, these assays are conducted by measuring the amount of released Chromium, calcein AM, or LDH molecules after the target cancer cells are killed with CAR T cells. These methods require a large amount of target cells which may not be ideal when working with donor primary samples. Additionally, they cannot [...]

A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy.     Download our white [...]

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