Video: Validating and optimizing single cell sorting of FACS using Celigo image cytometry
This presentation by John Tigges and Eric Zigon from BIDMC (Beth Israel Deaconess Medical Center, Harvard Medical School) includes an introduction to understanding the accuracy and efficiency of your FACS sorter, current method for validating single cell sorting, and Celigo image cytometry method, followed by initial single cell sorting validating using beads, validation of single cells in 96-well plates using Celigo, and finally a summary.