Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer
Alterations in apoptosis are relevant to a large number of disease states, including cancer, heart disease, neurological disorders, infectious disease, and more [1]. One of the earliest steps in the apoptosis pathway, the loss of mitochondrial membrane potential, is often an indication of disorder and is, therefore, a useful target for those investigating the mechanism of disease in vitro. Mitochondrial membrane potential can be easily and efficiently monitored using a number of commonly available fluorescent dyes.