Nexcelom Bioscience


User Feedback on Cellometer Auto 2000

We really like our Cellometer Auto 2000! The faster, move accurate cell counts have improved our data and analysis! - Leila Mulder, Colorado State University
We love using our Cellometer Auto 2000 with the live/dead assay to determine viability! - Steven Frisch, West Virginia University
The Cellometer Auto 2000 from Nexcelom is very robust and useful for counting PBMCs. I have used Cedex and Vi-Cell in the past, which took a lot of time while waiting for samples to be counted. With the Auto 2000, my cells don't need to sit in a tube while waiting for cell count results!
I have just joined the Immune Monitoring lab at Human Longevity and they have the Cellometer Auto 2000 and it is amazing! It cuts cell counting from about 30 minutes down to about 2 minutes or so! This really speeds up the down stream applications! I have used it for both primary and immune cells with equal success and reliability. - Cheryl Andreoli, Human Longevity, Inc
We love the Cellometer Auto 2000! It is very effective, convenient and saves my time! It is very easy to use with the touch screen!
The Cellometer Auto 2000 gives very reliable cell counts, much superior to the competition! - Satinder Dahiya, CHOP
We have been using the Cellometer Auto 2000 to count splenocyte samples without RBC lysis. The Cellometer Auto 2000 and the AOPI Viastain gives us the ability to get an accurate count of our live splenocytes while excluding the red blood cells.
The Cellometer Auto 2000 RULES! Whether I have one sample or 50 samples to count, it is fast and accurate. Almost no matter what your cell concentration, the Auto 2000 will give you the concentration. And not only do you get live cell count, you also get percent viability and cell diameter. I use the Auto 2000 every time I count! - Amy Coddington, Kyowa Kirin Research
The Cellometer Auto 2000 has changed the way we count cells. It saves time and takes away worries about inaccuracy! We can do a lot more in a day because of this machine.
The Cellometer Auto 2000 has a beautiful interface that is easy to use. We process whole blood, and our viability counts have gone up now that the RBCs are excluded.
The Cellometer Auto 2000 is very reliable and easy to use! Counting cells is much simpler with this instrument!
The Cellometer Auto 2000 has provided a robust method for counting cell samples without tech-tech variability. It's great! - Victoria Groysberg, UCLA Health
The Cellometer Auto 2000 counter has been an incredible addition to our workflow at UCLA Immunogenetics Center. We routinely count PBMCs and cryopreserve them at a specific number per vial. Since adding the Auto 2000 to the lab, our samples have become more reliable in terms of viability and accuracy of counts. I would highly recommend this to any biorepository lab! - Tiffany Sidwell, UCLA
The Cellometer Auto 2000 makes it easier to get an accurate count on my cells compared to flow cytometry. I am comfortable with it and trust the data that I obtain!
The Cellometer Auto 2000 has changed the way my lab works! It is a streamlined workflow and is extremely user friendly!
Our Cellometer Auto 2000 has increased the speed and ease of cell counting. Using it with the AOPI dye has made our cell count and viability measurements much more consistent and accurate than Trypan Blue for morphologically varied tissue homogenates (e.g. mouse small intestines).
The Cellometer Auto 2000 saves so much time! Experimental data is a lot more reliable when using the Cellometer Auto 2000 than trying to count lavage cells from 20 different samples on hemacytometer.
The Cellometer Auto 2000 is easy to program, troubleshoot, and use. It is so fast and user friendly that it speeds up the research process tremendously! - Renata Linardi, UPENN
The Cellometer Auto 2000 has greatly aided the development of our tissue dissociation assays. The unit allows fast quantification of viable white blood cells from a heterogeneous population following spleen, thymus, lymph node, etc. dissolution which can then quickly proceed to downstream flow-based analysis. The time saved by this method has been extremely valuable! - Stephen Grossman, Takeda Pharmaceuticals
We use the Cellometer Auto 2000 daily to count cells and assess viability of cells derived from a variety of sources. For example, we perform a lot of cell line work, and need accurate counts and viability assessments before using the cells in downstream assays. We work with frozen human PBMC cells as well and require accurate counts and, even more importantly, detailed viability information on the cells that we have thawed, cultured or manipulated in some way. Thus, we use the AOPI reagent to get very accurate counts and viability measurements on our cultured/manipulated/stimulated cells. I've personally compared the results using our Cellometer vs. other counting instruments, and have concluded that the Cellometer is superior to all other devices tested! - David DiLillo, Regeneron Pharmaceuticals
The Cellometer Auto 2000 has streamlined our Biorepository Core PBMC counting procedures allowing for a quicker, more precise and more reliable/reproducible cell count. The Auto 2000 has become a cornerstone to our daily Biorepository/ Bio-banking process! - Joseph Keir, Oklahoma medical Research Foundation
My colleague and I have been using the Cellometer Auto 2000 for a year now and absolutely love it! We are doing in vitro stimulation culturing of PBMCs followed by both intracellular cytokine staining and Elispot. The cell counter has facilitated and streamlined our work. Upon receiving the PBMCs, we generally count and then begin culturing the cells under differing growth conditions. After a 14-day culture, we harvest the cels and then set up our assays. The Auto 2000 makes the counting step so much easier! While the differing cultures are in the final wash/spin, we count the cells. This generally takes about 90 seconds for each growth condition, so that by the time the last spin is done, we already know the cell count and have determined what volume they need to be re-suspended in to achieve a linear cell concentration within our assays. No more looking in the microscope and trying to determine whether a cells is dead or not, whether it is a lymphocyte or erythocyte, etc. And the results are consistent! - Chris Elly, Human Longevity, Inc
The Cellometer Auto 2000 is very accurate, easy to use, and there is no need to repeat your count! - Hui Xu, HD Biosciences
The Cellometer Auto 2000 has greatly increased our productivity for in vitro experiments. The automated counting saves hours of time each week compared to manual counting with a hemacytometer! Also being able to quickly access viability of cells is great!
I like using the Cellometer Auto 2000 when counting T cells stained with AOPI. - Bonnie Le, TCR2
The Cellometer Auto 2000 works well, and it has helped speed up the cell counting process compared to manually counting.
The Cellometer Auto 2000 has made functional testing of T cells a much quicker and simpler process than before! When the cell concentration of each population in a 96 well plate must be determined, it is hard to imagine having to count the cells by hand and doing this in a timely manner. Great product!
The Cellometer Auto 2000 is a must-have piece of equipment in the lab! It makes life easy and saves a lot of time counting cells. It's easy to use and very portable piece of equipment which does not require a lot of space in the lab. If and when a technical issue arises, the tech team will eagerly support you. The team does not hesitate to visit your lab right away if they are in the vicinity of your lab. If not, they will immediately make arrangements to visit you to resolve your issue at the earliest. This is one company, to my experience, that stands by you and their product at all times. - Sanat Dave, UT MD Anderson Cancer Center
I like working with the Cellometer Auto 2000 because I can get quick, consistent results! Before the Auto 2000, our lab was manually counting cells with a hemacytometer. Manual counting can be time-consuming and inconsistent. As a result of this time-saving equipment, we can focus more of our time and energy on what matters most - our research!
I like the Cellometer Auto 2000 in general, I like the accuracy and the ability to use it for different cell types!
On the Cellometer Auto 2000, the AOPI stain is great for routine cell counting, and much more accurate than Trypan Blue. I am grateful the touchscreen is not capacitive, as we wear gloves when using it. - Luis Rodriguez, Regeneron Pharmaceuticals
I use the AOPI viability dye with the Cellometer Auto 2000 to count primary murine and human cells, as well as cell lines. The cell counts are more accurate than traditional Typan Blue method.
We are addicted to using the Cellometer Auto 2000 over grid counting cells. The instant calculation of dilutions, cell diameter, cell viability and a count in each population has helped us tremendously.
The ability to use AOPI dyes in cell counting has greatly increased our ability to count potentially difficult cell populations, such as PBMCs. When the separation has a higher amount of debris present, I feel that I am able to get a more accurate count using this machine, rather than just with Trypan Blue stains!
No more second guessing when counting cells as with the hemacytometer. The Cellometer Auto 2000 is fast, easy, and most of all - accurate! The computer even counts the volume you need for future experiments. The images can be saved as a reference too. The Cellometer makes the whole process very enjoyable! - Carole Grenier, Duke University Medical Center
I did my Postdoctoral work in the Kipins Lab at UVA. As part of a well-funded group looking to do cutting-edge science, I was involved in a lot of the purchasing decisions. We had a gorgeous confocal, a 2-Photon microscope, great FACS capabilities, you name it --- but I swear the best thing I recommended was our Nexcelom cell counter! The Cellometer Auto 2000 was easily the most-used piece of equipment in the lab. Super-reliable, accurate, and fantastic support on the rare occasion we needed something adjusted. Now that I've moved on to the next phase of my career, I did not even want to see machines from anyone else. HIGHLY recommended! - Noel Derecki, Janssen
The Cellometer Auto 2000 has been a huge help to our lab! Before purchasing, we used to count on the old fashioned glass hemacytometer, always with some variability. Now, all that we do is mix our cells with the AOPI, put them on the slide, and count. It has been great help!
With the Cellometer Auto 2000, our cell viability assay take about 25% of the time it used to take using flow cytometry!
The lab I am in uses the Cellometer Auto 2000. It is so much faster than manually counting cells, and the count is every bit as accurate. It is extrememly easy to use and saves everyone in the lab a lot of time!
We use our Cellometer Auto 2000 daily for our cell culture counting and viability assays. It's very easy to use, with user-friendly software, quick and accurate cell counting, proper record keeping, and cheap maintenance. It's a small treasure for our GMP lab and I highly recommend it!
We just recently purchased the Cellometer Auto 2000 system and routinely use it for mesenchymal stem cell count and viability. I like that it's fully automated, and all the analysis takes less than a minute! It's more accurate than to manually count with a hemacytometer.
The speed and simplicity of the Cellometer Auto 2000 is great. Such a time-saver over the old-fashioned hemacytometer. Easier and less messy to use than the Beckman Vi-Cell.
The Cellometer Auto 2000 is a great cytometer! It works well counting PBMCs isolated from human blood. We tested the counts in comparison to our old cytometer and by counts made by eye, and the numbers were accurate and reliable!
Our Cellometer Auto 2000 has been a staple in multiple of our projects. We use it on a daily basis and have been very happy with its performance. It is reliable and a huge time saver!
I have used different models of the Nexcelom Cellometers in various labs and it is definitely the standard for cell counting. Results are consistent time and again, the reliability of which is great when performing duplicate or triplicate counts. One of the best things about the Cellometer Auto 2000 in particular is the ability to distinguish dead cells from live ones based on different color staining. It allows for visual confirmation of what the machine is already telling us, which is great when it comes to really low cell counts that can be quite demoralizing. I also enjoy the ability to establish several different settings based on the type of cell, and also appreciate Nexcelom's customer service! -Melissa Yao, Cold Spring Harbor Laboratory
The Cellometer Auto 2000 is very reliable in operation. It responds quickly between functions and usages without rebooting. It has great consistency between different functions/modes in cell counting.
Thanks to the Cellometer Auto 2000, I can do my flow cytometry and HSC transplantation faster and better, while giving a very repeatable reading!
I absolutely trust the Cellometer Auto 2000 cell count results for primary and immune cells. It's easy to use and provides the accuracy I need for my experiments. It is one of the best lab purchases! All I know is that I'm never counting primary cells manually again! The customer service is great as well! -Joeseph Castillo, Genentech
Our Cellometer Auto 2000 has greatly improved the speed and accuracy of our cell counting and has allowed us to analyze our cell samples in a variety of ways. The AOPI feature has been integral in generating cell viable data for a few of our new projects. The customer service team is also amazing! They are very knowledgeable about their products and technology and are always available to help us troubleshoot.
The Cellometer Auto 2000 is a very efficeint way to quickly count cells. We have been using it for every cell culture experiment and pass, making that step of the process pretty seamless.
The Cellometer Auto 2000 system has greatly simplified the way my company does cell counting and has become a very essential piece of equipment. We use it for both high and low RBC cell counting for viability purposes to accurately culture large volumes of cells. It has cut down on the amount of time we take to do cell counts and allows us to complete our processes in a very timely fashion. Truly a great product! - Derek Grice, Cognate BioServices, Inc.
We purchased a Cellometer Auto 2000 cell viability counter. It is great for cell counting and viability recordings. The touch screen feature and cell imaging features are great.
My colleague and I have purchased a Cellometer Auto 2000 cell counter back in January of this year. We are using it now and it has facilitated our work greatly. We routinely process PBMCs from both fresh whole blood and from frozen stock. The Cellometer has made it much easier to get cell numbers and viability percentages for use in downstream applications such as IVS and Elispot. -Christian Elly, Human Longevity, Inc
I would cry if I had to work without the Cellometer Auto 2000. -Victoria Groysberg, UCLA
Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom! -Tiffany Sidwell, UCLA Immunogenetics Center
Our lab bought a Cellometer Auto 2000. We regularly grow primary human T cells, and with a lot of samples per experiment (20-30+), it has been a lot of work to keep track of growth/expansion and viability prior to having the Cellometer. With the Auto 2000, it is much easier, and much more reliable to count each set of samples and get reliable data on expansion and viability, and we are able to take samples for counts every other day, rather than once a week.
I have used the Cellometer Auto 2000 in my previous lab extensively for counting mouse primary cells. I cannot be thankful enough to you guys for making such a great product. I liked it so much that I convinced my boss at my new lab to buy the Cellometer Auto 2000 in spite of already having a cell counter from different company.
I like the Cellometer Auto 2000 because it eliminates manual counting and our counts are consistent between users. We count cells from primary samples and I like that the RBCs are not counted when we use the AO/PI stain. - Jeff Painter, Moffitt Cancer Center
Using the Cellometer Auto 2000 has been a walk in the park! The instrument is flawless and the digital display is exquisite! This machine has saved me so much time and has allowed me to complete all my work in a timely fashion. Thanks guys! Keep up the good work! - Dylan Johnson, Burleson Research Technologies
The Cellometer Auto 2000 has made it possible to work faster, more efficiently and with more precision. - Maria Agarwal, Johns Hopkins University
I like my Cellometer Auto 2000 because it saves my images that I may go back and review. I also like the histogram and the ability to export to Excel.
We have the Cellometer Auto 2000. This instrument has streamlined our experiments. We have several animal studies that require various cell counts. Given that these studies take place over several months, the Cellometer has given us a mode by which to keep out counts consistent (as opposed to manual counting, which would require the same person to count over the course of experiments and is relatively subjective anyhow). We love how quickly we can obtain our data and feel confident that the instrument is giving us accurate results. It's also convenient that the Cellometer works with a variety of dyes (AO/PI, Trypan Blue, etc.).
We purchased a Cellometer Auto 2000 in late May and have been very pleased with the way it has already changed the work-flow for almost all of our cell-based projects. We are finding it much easier to generate quantitative data that we trust at a rate of progress well beyond what was possible in our lab before by conventional imaging and image analysis approaches. - Rob Bellin, Holy Cross College

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