Cell Counting Archives | Page 4 of 5

Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells

Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]

By Polly Mitchell|2021-06-15T19:14:39+00:00February 4th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: AO/PI, Cell Counting, Cellometer Auto 2000, UCMSCs, umbilical cord cells|0 Comments

Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale

Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]

By Polly Mitchell|2021-06-15T19:16:05+00:00January 19th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: Cell Counting, Cell Viability, Cellometer Auto 2000, hMSC, regenerative medicine|0 Comments

Cellometer helps with culturing optimization necessary for future cell-based therapies

EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]

By Polly Mitchell|2021-06-15T19:16:26+00:00January 14th, 2016|Categories: CAR T / Cell Therapy, Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, cell-based therapy, Cellometer, mesenchymal stem cells, Stem Cell|0 Comments

Cellometer T4 work in Adoptive Cell Transfer examines gender differences in pathology after stroke

Oregon Health & Science University researchers continued their studies into the gender-related differences in peripheral immune system response after stroke that produce greater downstream damage in males than females, and what role the spleen may play in those differences. The goal of this work was to uncover which subset of immune system cells may contribute to those pathogenic effects after a stroke and how they vary by gender. Using primary leukocytes from transgenic mice and adoptive T-cell transfer, scientists injected specific cell types into splenectomized male and female mice 24 hours after stroke was induced. The Cellometer Auto T4 maintained [...]

By Polly Mitchell|2021-06-15T19:17:51+00:00January 7th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, Cellometer Auto T4, immune system, stroke|0 Comments

Cellometer Auto T4 evaluates the cytotoxic potential of mouth rinses

How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]

By Polly Mitchell|2021-06-15T19:18:14+00:00January 5th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, cell size, Cell Viability, Cellometer Auto T4, gingiva fibroblasts, mouthwash, Viability|0 Comments

Cellometer assists in search to combat tumor radio-resistance

At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]

By Polly Mitchell|2021-06-15T19:18:34+00:00December 29th, 2015|Categories: Cellometer, Cellometer Application News, Instrument, Oncology / Tumor Microenvironment|Tags: Cell Counting, Cellometer, Trypan Blue|0 Comments

Cellometer assists in characterization of adipocytic extracellular vesticles

Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]

By Polly Mitchell|2021-06-15T19:43:21+00:00December 17th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: adipocytes, Cell Counting, Cellometer, obesity, Trypan Blue|0 Comments

Cellometer Used in study to Establish Baseline Values of Autism Biomarkers in Saliva

University of Minnesota scientists analyzed the saliva of twelve “neurotypical” adult subjects for the levels of sixteen autism-associated biomarkers (glutamine, glutamic acid, CD-26, C4B, IFN-γ, MT-2, testosterone, IL-12, Carnitine, GSH, GSSG, cystine, GABA, serotonin, cortisol and melatonin) in the hopes that establishing baseline levels of these molecules in a control population might help identify autism-associated changes in non-neurotypical subjects in the future. The Cellometer was used to establish accurate cell counts throughout the experiment. Ten biomarkers were successfully measured and the sample collection protocol proved to be non-stressful and easy enough to use on a broad range of subjects. You [...]

By Polly Mitchell|2021-06-15T19:45:57+00:00November 18th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autism, Cell Counting, Cellometer|0 Comments

Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation

It’s White Paper Wednesday! This month’s featured white paper: Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation The hemacytometer persists as the gold standard for laboratory cell counting. First utilized in 18th century France as a means to analyze patient blood samples, the hemacytometer has gone through a series of major developments over the past hundreds of years, creating a modern instrument that is more accurate and easier to use than its predecessors. The hemacytometer remains an integral part of all cell-based research, and yet sources of error inherent in its design and utilization persist. Those sources [...]

By Leo Chan|2021-06-15T20:36:59+00:00November 12th, 2014|Categories: Cell Counting Leadership, Cellometer Application News|Tags: Cell Counting, hemacytometer, Hemocytometer|0 Comments

Rapid Image Cytometry Method for Cell Counting and Viability Measurement for Cellular Therapy

Patients with cancers such as multiple myeloma, leukemia, lymphoma, and other metastatic cancers have tumor cells with unique immunological targets that when exploited, can lead to the complete destruction of the mass and a lasting remission from disease. Clinicians attack those targets using cell therapy, also known as “targeted immunotherapy”. Cell therapy is the programming of a patient’s own immune cells to target that patient’s tumor cells for destruction. One application of cell therapy, Adoptive Cell Transfer (ACT), employs select methods of genetic manipulation and propagation of those immune cells ex vivo so that the newly programmed cells can be [...]

By Leo Chan|2021-06-15T20:37:41+00:00October 13th, 2014|Categories: CAR T / Cell Therapy, Cellometer Application News, Instrument|Tags: Cell Counting, Cellular Therapy, image cytometry|0 Comments