Exploration of candidate genes responsible for esophageal squamous cell carcinoma (ESCC) pathogenesis may provide insight into the underlying signaling pathways to uncover novel therapeutic targets.
The ATR-CHK1 pathway plays a critical role as a replication checkpoint and is a promising target for emerging therapies.
Starting with a bioinformatics approach using publicly available databases, the authors were able to confirm that tissues from patients with lung adenocarcinoma had increased expression of BCCIP and that high expression levels of BCCIP were correlated with poor patient prognosis.
Solid tumor biology has become a critical step in accessing the potency of many cancer drugs. 3D spheroids and organoids can provide measurements such as size differences under drug conditions or metabolism and diffusion rates critical to drug development.
The development of targeted molecular therapies is an increasingly popular approach to combat and overcome the limitations of conventional treatments.
A fundamental understanding of the molecular mechanisms responsible for the development and progression of RCC will be pivotal in developing next-generation treatment protocols to improve patient outcomes.
During this webinar we will highlight a variety of assays to study the tumor microenvironment
At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]
Nexcelom has performed Cellometer image cytometry analysis on all NCI-60 cancer cell lines.
Establishment of Human Ultra-Low Passage Colorectal Cancer Cell Lines Using Spheroids from Fresh Surgical Specimens Suitable for In Vitro and In Vivo Studies
Human primary colon cancer cells digested from tumor used to establish ultra-low passage colorectal cancer cell lines. Cell count and viability were determined by the trypan blue dye exclusion method using a T4 Cellometer (Nexcelom Bioscience LLC, USA). […]