Cellometer User Publications Archives | Page 4 of 5

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By Leo Chan|2021-06-15T20:35:02+00:00January 29th, 2015|Categories: Cellometer User Publications, Immuno-oncology|Tags: Apoptosis, Cell Cycle, Cellometer Vision, Ovarian Cancer|0 Comments

Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

By Leo Chan|2021-06-15T20:35:18+00:00January 22nd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autophagosomes, Autophagy, Cell Cycle, Cellometer Vision, Cyto-ID, Proliferation|0 Comments

Cellometer Vision CBA image cytometry reveals the utility of the immunocompetent mouse model “Glowing Head” in assessing preclinical anti-tumor therapies

Introduction Preclinical models to evaluate therapeutic efficacy in the area of oncology have proven to be a challenge. The standard of preclinical assessment as always been immunosuppressed mice, but that model has not reliably predicted clinical efficacy [1]. Immunocompetent genetically engineered mice with tumor allograft models seem more promising, and yet reporter tags such as GFP and luciferase are targeted by competent immune systems, which may interfere with tumor growth and the response to therapeutics. Here, a collaborative group of researchers has created a reporter-tolerized genetically engineered mouse model, dubbed “Glowing Head” (GH), by targeting a luciferase-GFP reporter into the [...]

By Leo Chan|2021-06-15T20:35:29+00:00January 15th, 2015|Categories: Animal Models, Cellometer, Cellometer User Publications, Instrument|Tags: anti-tumor therapies, Cellometer Vision CBA, Glowing Head, Luciferase-GFP reporter|0 Comments

Iron oxide-based nanoparticles conjugated NuBCP-9 increase MCF-7 apoptosis as assessed by Cellometer Vision CBA Image Cytometer

Introduction The B-cell lymphoma-2 (BCL-2) family of proteins includes many key regulators of cell survival, cell death, and apoptosis. The deregulation of certain BCL-2 proteins is one of the first steps towards tumorigenesis and subsequent therapeutic resistance [1-4]. Targeting these BCL-2 family members is therefore an important area of oncology research [2, 4, 5]. Here, a collaboration of researchers has explored the targeting of these BCL-2 proteins with a BCL-2 binding peptide known as NuBCP-9 (FSRSLHSLL), which induces a conformational change in BCL-2 that alters the protein’s function from cell protection to cell killing. Researchers paired NuBCP-9 with iron oxide-based [...]

By Leo Chan|2021-06-15T20:35:46+00:00January 8th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Apoptosis, Cellometer Vision CBA|0 Comments

Evaluation of Nanoparticle-Based Therapy for Triple-Negative Breast Cancer using Cellometer Vision CBA

Introduction to Nanoparticle-Based Therapy for Triple-Negative Breast Cancer Tia Harmon and Dr. Ruben Gonzalez-Perez from the Morehouse School of Medicine and Emory University utilized the imaging and analysis capabilities of the Cellometer Vision to investigate leptin peptide receptor antagonist-conjugated nanoparticles in order to inhibit leptin signaling, a key pathway that promotes growth and survival of triple-negative breast cancer (TNBC) cells. It was hypothesized that these nanoparticles would first stop the expression of leptin’s downstream target Notch, and would thereby increase the effectiveness of standard chemotherapeutic compounds in limiting tumor cell survival [1-4]. Materials and Methods A human ER+ cell line (MCF-7) [...]

By Leo Chan|2021-06-15T20:36:25+00:00December 8th, 2014|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|0 Comments

Establishment of Human Ultra-Low Passage Colorectal Cancer Cell Lines Using Spheroids from Fresh Surgical Specimens Suitable for In Vitro and In Vivo Studies

Human primary colon cancer cells digested from tumor used to establish ultra-low passage colorectal cancer cell lines. Cell count and viability were determined by the trypan blue dye exclusion method using a T4 Cellometer (Nexcelom Bioscience LLC, USA). […]

By Polly Mitchell|2021-06-15T20:48:29+00:00July 27th, 2012|Categories: Cellometer User Publications, Oncology / Tumor Microenvironment|0 Comments

Triptolide Inhibits the Proliferation of Prostate Cancer Cells and Down-Regulates SUMO-Specific Protease 1 Expression

LNCaP and PC-3 cells Cells were gently trypsinized and staining with trypan blue dye. The viable cells were counted using cell counting chamber every 24 h for 7 days. 48 h after transfection, cells were treated with Triptolide for another 48 h. After stained with trypan blue, viable cells were counted using cellometer Auto T4 automated cell counter. […]

By Polly Mitchell|2021-06-15T20:48:38+00:00July 27th, 2012|Categories: Cellometer User Publications, Oncology / Tumor Microenvironment|0 Comments

Telomerase Efficiently Elongates Highly Transcribing Telomeres in Human Cancer Cells

To analyze cell viability, cells were stained with 0.2% trypan blue (Sigma) and counted using the Cellometer Auto T4 instrument (Nexcelom Bioscience). […]

By Polly Mitchell|2021-06-15T20:48:46+00:00July 27th, 2012|Categories: Cellometer User Publications, Oncology / Tumor Microenvironment|0 Comments

Down-Regulation of Yes Associated Protein 1 Expression Reduces Cell Proliferation and Clonogenicity of Pancreatic Cancer Cells

BxPC-3, PANC-1, and HPDE6 cells were washed with DPBS, trypsinized, and then counted on Cellometer AutoT4 (Nexcelom Bioscience, Lawrence, MA) […]

By Polly Mitchell|2021-06-15T20:49:02+00:00July 27th, 2012|Categories: Cellometer User Publications, Immuno-oncology|0 Comments

Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium

A portion of the GFP-labled or unlabeled UCB MSCs (umbilical cord blood mesenchymal stem / stromal cells) were incubated with trypan blue and counted using a Cellometer Auto T4 cell counter (Nexcelom Bioscience LLC, Lawrence,MA) to determine the number of viable cells. […]

By Polly Mitchell|2021-06-15T20:49:12+00:00July 27th, 2012|Categories: Cellometer User Publications, Stem Cell/Regenerative Medicine|0 Comments