Cellometer Archives | Page 4 of 5

Cellometer assists in search to combat tumor radio-resistance

At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]

By Polly Mitchell|2021-06-15T19:18:34+00:00December 29th, 2015|Categories: Cellometer, Cellometer Application News, Instrument, Oncology / Tumor Microenvironment|Tags: Cell Counting, Cellometer, Trypan Blue|0 Comments

Cellometer used in study to examine secondary effects of MAO inhibitors

In the “catecholaldehyde hypothesis” of Parkinson’s disease neurodegeneration, cytoplasmic dopamine (DA) is converted to 3,4-dihydroxyphenylacetaldehyde (DOPAL) by the enzyme monoamine oxidase-A (MAO-A). DOPAL is a toxic substance, generating free radicals and inhibiting mitochondrial function. Scientists at NIH wanted to compare the abilities of currently available MAO-A and MAO-B inhibiting drugs at decreasing endogenous DOPAL levels. Using PC12 cells, six drugs were tested for their effects on DOPAL: MAO-A inhibitor clorygyline, three reversible MAO-A inhibitors, and the MAO-B inhibitors selegiline and rasagiline. The Cellometer was used to count the cells throughout experimentation. Clorgyline, rasagiline, and selegiline decreased DOPAL levels in both [...]

By Polly Mitchell|2021-06-15T19:43:09+00:00December 22nd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer, MAO inhibitors, NIH, Parkinson's|0 Comments

Cellometer assists in characterization of adipocytic extracellular vesticles

Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]

By Polly Mitchell|2021-06-15T19:43:21+00:00December 17th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: adipocytes, Cell Counting, Cellometer, obesity, Trypan Blue|0 Comments

Cellometer participates in study to ascertain rotenone mechanism of action

A common hypothesis concerning the pathogenesis of Parkinson’s neurodegeneration suggests that cytoplasmic dopamine (DA) is converted to the auto-toxic 3,4-dihydroxyphenylacetaldehyde (DOPAL), which is neutralized by aldehyde dehydrogenase (ALDH). NIH researchers studied whether rotenone, a substance typically used to create in vitro models of Parkinson neurodegeneration, inhibits ALDH function. PC12 cells were exposed to various concentrations of rotenone in the presence or absence of F-dopamine. Catecholamine metabolism was then measured. The Cellometer maintained cell counts throughout experimentation. Rotenone increased DOPAL while decreasing dopamine levels in cell culture, but in a test tube, the DOPAL to DOPAC conversion by ALDH was not [...]

By Polly Mitchell|2021-06-15T19:43:33+00:00December 15th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer, NIH, Parkinson's|0 Comments

Cellometer helps to elucidate underlying mechanism of hypomyelination

Vanderbilt University researchers sought to evaluate the role of mTor signaling during myelination. Other work suggests that myelin abnormalities may be distinct from cortical lesions, and these scientists investigated the underlying mechanisms of hypomyelination to support or refute the other data. Primary astrocytes from knock out mouse pups were isolated to ascertain whether the astroglyosis seen in these mouse models is a specific or non-specific consequence of altered oligodendrocyte structure. Cellometer analyzed spherical size of these cells during experimentation. Although the findings suggest it is a nonspecific response, more study is clearly needed. These findings also suggest a dissociation between [...]

By Polly Mitchell|2021-06-15T19:43:40+00:00December 10th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: cell size, Cellometer|0 Comments

Cellometer investigates new treatment paradigm for advanced endometrial cancer

University of North Carolina researchers investigated different techniques for inhibiting the catalytic activity of protein hTERT – a marker of advanced stage endometrial cancer. Endometrial cancer cell lines ECC-1 and Ishikawa were exposed to either siRNA or a small molecule pharmacological inhibitor BIBR1532, in addition to the drug paclitaxel, to see whether inhibiting hTERT provided additional efficacy against these cancer cells. The Cellometer, in combination with propidium iodide and Annexin-V FITC, calculated apoptosis in the various treatment conditions. The hTERT inhibition plus paclitaxel did prove synergistic, reducing cell growth and invasion more than paclitaxel alone. Furthermore, BIBR1532 antagonized cell invasion [...]

By Polly Mitchell|2021-06-15T19:44:51+00:00November 23rd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Annexin-V, Cancer Cell Lines, Cellometer, Image Cytometer, image cytometry, Imaging Cytometer, Imaging Cytometry, Propidium Iodide|0 Comments

Cellometer Used in study to Establish Baseline Values of Autism Biomarkers in Saliva

University of Minnesota scientists analyzed the saliva of twelve “neurotypical” adult subjects for the levels of sixteen autism-associated biomarkers (glutamine, glutamic acid, CD-26, C4B, IFN-γ, MT-2, testosterone, IL-12, Carnitine, GSH, GSSG, cystine, GABA, serotonin, cortisol and melatonin) in the hopes that establishing baseline levels of these molecules in a control population might help identify autism-associated changes in non-neurotypical subjects in the future. The Cellometer was used to establish accurate cell counts throughout the experiment. Ten biomarkers were successfully measured and the sample collection protocol proved to be non-stressful and easy enough to use on a broad range of subjects. You [...]

By Polly Mitchell|2021-06-15T19:45:57+00:00November 18th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autism, Cell Counting, Cellometer|0 Comments

Researchers in Turkey use Cellometer for Trypan Blue Viability

Here’s another great example of how the Cellometer instruments are used to test viability of cells prior to plating. Standard therapy zoledronic acid (ZA) is typically used for metastatic bone disease in breast cancer patients, but it’s mechanism of action is not clear. Here, Turkish scientists investigated zoledronic acid with and without serine-threonine inhibitors in human MCF-7 and MDA-MB-231 breast cancer cells. Cellometer was used in combination with tryan blue to assess cell viability prior to plating. After plating, the cells were treated with various combinations of therapeutic agents. ZA with either one of the serine-threonine inhibitors examined proved more [...]

By Polly Mitchell|2021-06-15T19:46:40+00:00November 10th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Breast Cancer Drugs, Cellometer, Trypan Blue|0 Comments

UNM College of Pharmacy uses Cellometer to perform TB and AO/PI Viability

Here's a great example of how the Cellometer is being used by researchers in toxicology. The University of New Mexico College of Pharmacy investigated the mechanisms by which environmentally-relevant concentrations of inorganic arsenic contribute to genotoxicity. Inorganic arsenic is present in the air, food, and drinking water in many countries and has been linked to various cancers, skin lesions, and immune system disorders, although the exact mechanisms are not yet clear. To study arsenic’s genotoxic mechanisms, the researchers employed Nexcelom’s Cellometer to assess cell number and viability of primary mouse thymus cells and the murine thymus pre-T cell line, D1 [...]

By Polly Mitchell|2021-06-15T19:48:26+00:00October 19th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer, toxicology, Trypan Blue|0 Comments

Cellometer Training Webinars available on YouTube!

Nexcelom hosts free, live monthly training webinars for our Cellometer instruments. Our webinars cover a variety of instruments, cell types and applications. We have taken our recorded webinars and added them to our YouTube channel! We've created a playlist entitled "Cellometer Training Webinars" and the list will continue to grow as we release more. This is a great resource for you and new lab team members to get additional training on your Cellometer instrument. We host new training webinars each month, so revisit our YouTube channel from time to time to see new content as it's produced and added.

By Polly Mitchell|2014-12-10T10:41:41+00:00December 10th, 2014|Categories: Cellometer Application News|Tags: Cellometer, Cellometer Auto 1000, Cellometer Mini, Cellometer Vision, Cellometer X2, training, webinar|0 Comments