Antibody-Dependent Receptor Internalization Assay
To measure the level of receptor internalization induced by known antibody
HPC proliferation measurement using Ki-67 cellular marker
Celigo will be able to perform rapid, whole-well imaging and analysis of Ki-67 expression levels to compare between groups.
Kinetic apoptosis using Caspase 3/7
By measuring the number of Caspase 3/7 positive cells, we can determine the counts of apoptotic cells in the population
Kinetic viability using PI
Determine the counts of PI-positive cells kinetically at 24, 48 and 72 hours
Endpoint Viability using Propidium Iodide and Hoechst
Perform endpoint viability assay on MDA-MB-231 and K562 cells treated with Benzethonium for 24, 48 and 72 hours
High-throughput suspension cell count and viability using AO/PI
Measure Jurkat cell concentration and viability at titrations of concentrations and different mixture of cell viability, which can demonstrate the high-throughput capability of Celigo image cytometer
Kinetic viability using DRAQ7
Drug treatment will increase the percentage of DRAQ7-positive cells over time
Endpoint viability using DRAQ7 and Hoechst
Perform endpoint viability assay on MDA-MB-231 and K562 cells treated with Benzethonium for 24, 48 and 72 hours
Endpoint apoptosis using Caspase 3/7 with Hoechst
By measuring the number of Caspase 3/7 positive cells, we can determine the percent of apoptotic cells in the population
Counting of Patient-Derived IDC Organoids
To image and count whole well populations of organoids in a rapid manner without having to take multiple, time consuming Z-stacked images