Antibody-Dependent Drug Uptake Assay
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations
To analyze transfection efficiency over a 4 day period when treated with various amounts of a transfection compound.
In this experiment, we demonstrate the capability of Celigo to measure cell count on microcarrier beads and infected cells positively staining for AlexaFluor488-labeled antibody again the viral protein.
To measure the level of receptor internalization induced by known antibody
Celigo will be able to perform rapid, whole-well imaging and analysis of Ki-67 expression levels to compare between groups.
By measuring the number of Caspase 3/7 positive cells, we can determine the counts of apoptotic cells in the population
Determine the counts of PI-positive cells kinetically at 24, 48 and 72 hours
Perform endpoint viability assay on MDA-MB-231 and K562 cells treated with Benzethonium for 24, 48 and 72 hours
Measure Jurkat cell concentration and viability at titrations of concentrations and different mixture of cell viability, which can demonstrate the high-throughput capability of Celigo image cytometer
Drug treatment will increase the percentage of DRAQ7-positive cells over time