Nexcelom Bioscience
Nexcelom Videos

Nexcelom Video Library

Welcome to the Nexcelom video library. Our collection of videos is always being updated so be sure to check back for the latest.


Cellometer Celigo

Celigo S Image Cytometer Videos

Video: Celigo Imaging Cell Cytometer

Introduction to the Celigo Image Cytometer

Celigo S Image Cytometer

A multi-channel brightfield and fluorescence imaging cytometer for both attached and suspension cells.

Video: 3D Tumor Spheroid-Based Functional Assays - Invasion into Matrigel

3D Tumor Spheroid-Based Functional Assays - Invasion into Matrigel

Celigo Video Demo Series

Imaging and analysis of 3D tumor spheroid invasion into Matrigel using u-bottom 96-well ULA plates. Examine drug effects on tumor spheroids as a model system for tumor invasion.

Video: Celigo ADCC Assay

ADCC Assay by Direct Cell Counting

Celigo S Image Cytometer

A novel cytotoxicity assay using the Celigo imaging cytometer to directly count live, fluorescently labeled target cells has recently been introduced. Perform qualitative and quantitative analysis for ADCC, CDC, and cell-mediated cytotoxicity assays using a single platform.

Video: Celigo Plate Scan

Celigo Plate Scan

Celigo S Image Cytometer

Proprietary optics and scanning system enable fast imaging of entire well, while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.

Video: Celigo Key Features

Celigo Key Features

Celigo S Image Cytometer

The Celigo instrument has an easy work flow, image auto focus, four color acquisition, imaging & analysis of every cell to the well edge, fast image scanning & analysis, export data & images, and perform population histogram and 2D gating analysis.

Video: Celigo Key Features

Assay Show: Apoptosis Endpoint Assay

Celigo S Image Cytometer

This video will show you how to perform an apoptosis assay on the Celigo image cytometer using caspase 3/7 and Hoechst reagents

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Vision and Vision CBA Image Cytometry Videos (including cell-based assay demos)

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Quickly and Easily Measure Caspase-8 Activity

Cellometer Vision CBA Analysis System

Activation of Caspase-8 is the first step in the extrinsic apoptosis pathway. Though apoptosis is still reversible at this stage, Caspase-8 activation is frequently used as an early indicator of apoptosis.

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Measure GFP-Positive Cells to Determine Transfection Efficiency

Cellometer Vision CBA Analysis System

With the Cellometer Vision CBA, just 20┬Ál of sample is added to the Cellometer Counting Chamber. Imaging and analysis of GFP expression is completed in less than 60 seconds.

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Image-based Cell Cycle Analysis

Cellometer Vision CBA Analysis System

Perform cell cycle analysis with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain comprehensive plots and tables displaying cell population by phase. Easily switch between users and experiments with no set-up, washing, or potential clogging.

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Image-based Analysis of Apoptosis with Annexin V-FITC / PI

Cellometer Vision CBA Analysis System

Simplify apoptosis experiments with image-based analysis. Colorized data plots easily identify apoptotic and necrotic cells. Manually adjust gating with instant updates to data tables. View brightfield and fluorescent images for confirmation of results. Easily switch between users and experiments with no set-up, washing, or potential clogging.

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Assessment of Mitochondrial Membrane Potential using Image-Based Cytometry

Cellometer Vision CBA Analysis System

Perform apoptosis analysis based on changes in mitochondrial membrane potential with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain colorized scatter plots and data tables. Easily compare control and experimental samples using a simple, accurate, image-based method.

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Viability of Fragile Hepatocytes using Image-Based Cytometry

Cellometer Vision Analysis System

Primary hepatocytes are a critical component of studies involving liver disease, drug metabolism, and toxicology. Because hepatocytes do not proliferate in culture and display a rapid decline in viability and loss of liver-specific functions under sub-optimal culture conditions, the ability to monitor hepatocytes using a small number of cells is critical. Obtain live/dead cell count, concentration, mean cell diameter and % cell viability for hepatocytes in just 60 seconds from only 20µl of sample using a gentle, non-fluidic analysis method for the most accurate results.

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Measuring Live Cell Concentration and Viability of Human PBMC

Cellometer Vision Analysis System

Cellometer Vision is a line of simple to use, automated cell analyzers with diverse functions. It combines bright field microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. This video demonstration features the Cellometer Vision to measure live cell concentration and viability of human PBMC.

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Yeast Cell Concentration & Viability in Corn Mash

Cellometer Vision Analysis System

Cellometer Vision is a line of simple to use, automated cell analyzers with diverse functions. It combines bright field microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. This video demonstration features the Cellometer Vision to measure yeast cell concentration and viability in a highly viscous corn mash sample.

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Cellometer K2 and X2 / X1 Videos

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The Cellometer K2 Image Cytometer for Cell Counting and Analysis

Cellometer K2 Image Cytometer

This video shows an example of a viability measurement performed with the Cellometer K2. 20 micro liters of PMBC sample is mixed with Acridine Orange and Propidium Iodide solution and then loaded into the Cellometer counting chamber.

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The Cellometer X2 Image Cytometer for Cell Counting and Analysis

Cellometer X2 / X1 Image Cytometer

This video shows an example of a viability measurement performed with the Cellometer X2. 20 micro liters of yeast sample is mixed with Propidium Iodide solution and then loaded into the Cellometer counting chamber and analyzed.

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1-step Yeast Concentration & Viability Determination

Automatically generate yeast concentration and viability data from a single sample. See how brightfield and fluorescent imaging simplifies and enhances yeast fermentation monitoring with 1-click image capture and calculation of yeast concentration, % viability, and cell size. Save time and improve data quality and reproducibility vs. manual counting.

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Cellometer Auto X4: Measuring Algae Cell Concentration

Cellometer Auto X4 is a line of simple to use, automated cell counters with fluorescence detection. It combines bright field microscopy with fluorescence to identify selected cells. This video demonstration features the Cellometer Auto X4 to measure algae cell concentration from a pond water sample.

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Cellometer Auto 2000 Videos

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Cellometer Auto 2000 Cell Analyzer Demonstration

Cellometer Auto 2000 Cell Counter

This demonstration of the Cellometer Auto 2000 operation shows how quickly the automated system delivers total cell count, concentration and mean diameter from only 20µl of sample. Results also include a cell size histogram and percent viability.

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Overview of Cellometer Auto 2000 Assays

Cellometer Auto 2000 Cell Counter

Perform two-color fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. Accurately measure PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using sample-specific assays and a simple touch screen.

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Primary Cell Concentration & Viability using Cellometer Auto 2000 Cell Viability Counter

Cellometer Auto 2000 Cell Counter

Perform two-color fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. Accurately measure PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using sample-specific assays and a simple touch screen.

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Cellometer Auto T4, Auto 1000 and Mini Videos

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Cellometer Mini Cell Counter Demo

Cellometer Mini Cell Viability Counter

Demonstration of Cellometer Mini, the most affordable PC-based, automated 4x bright field for cell count, size, and Trypan Blue viability measurements of cell lines and purified primary cells.

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Cellometer Auto 1000 Cell Viability Counter Demo

Cellometer Auto 1000 Cell Viability Counter

Demonstration of Cellometer Auto 1000, the all-in-one, touch-screen instrument for automated 4x bright field analysis of cell count, size, and Trypan Blue viability in cell lines and purified primary cells.

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Improve Cell Counting Speed and Accuracy

Cellometer Auto T4 Cell Counter

Accurate cell concentration and viability calculations are critical for successful cell-based assays and flow cytometry analysis. The accuracy and reproducibility of the Cellometer Auto T4 Cell Counter have been demonstrated in thousands of labs, including the top ten pharmaceutical companies.

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Cellometer Assay Principle Videos

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Dual-fluorescent AO/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent AO/PI assay for cell concentration and viability. This assay is most suitable for primary cell samples which contain debris and unwanted non-nucleated cell types.

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Dual-fluorescent AO/EB Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent AO/EB assay for cell concentration and viability. This assay is most suitable for primary cell samples such as white blood cells, splenocytes and lymphocytes which contain debris and unwanted non nucleated cell types such as red blood cells.

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Dual-fluorescent CalceinAM/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent CalceinAM/PI assay for cell concentration and viability Calcein-AM penetrates the cell membrane of both living and dead cells. Once inside a living cell, Calcein-AM is hydrolyzed by cellular esterases into the cell membrane-impermeable Calcein. PI is only permeable to dead cells.

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Dual-fluorescent Fluorescein Diacetate (FDA)/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent FDA/PI assay for cell concentration and viability.

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Cellometer Feature Videos

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Save and Export Data

Cellometer Features

Keeping records of your research is critical. With the click of a button, Cellometer instantly saves counting results, cell images, graphs and data to a clipboard, local or network location.

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Sample Adjustment Calculator

Cellometer Features

The Cellometer Sample Adjustment Calculator is a unique feature that saves time and reduces human error. This tool automatically calculates the number of cells in the sample and allows you to change the original sample volume to meet desired target cell concentration.

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Cell Size Measurement

Cellometer Features

Unlike manual counting, Cellometer measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.

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Import Cell Type

Cellometer Features

The Cellometer import cell type feature uses the Nexcelom Cell Library that contains optimized counting parameters for more than 300 difference cell types. Users can import any selected cell type by browsing the Nexcelom Cell Library. Cellometer application specialist will optimize counting parameters for your cell type, free of charge.

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Cell Type Wizard

Cellometer Features

Cell type wizard is designed to automatically set up cell counting parameters for any new cell lines.

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