Nexcelom Bioscience

978-327-5340

Nexcelom Video and Webinar Library

Interested in seeing the Cellometer and Celigo in action? Here you can find product videos for our Cellometer automated cell counters as well as Cellometer and Celigo image cytometers. Product overviews as well as assay demonstrations are available. You can also learn about assay principals and Cellometer software features.

On demand informational webinars for both Cellometer and Celigo covering a wide range of topics are available to view on your schedule. View informational webinars »

Informational Webinars are regularly scheduled for both Cellometer and Celigo. Join us for one of our upcoming webinars »

Celigo Image Cytometer Videos

Video: Celigo Imaging Cell Cytometer

Introduction to the Celigo Image Cytometer

Celigo S Image Cytometer

A multi-channel bright field and fluorescence imaging cytometer for both attached and suspension cells.

Video: 3D Tumor Spheroid-Based Functional Assays - Invasion into Matrigel

3D Tumor Spheroid-Based Functional Assays - Invasion into Matrigel

Celigo Video Demo Series

Imaging and analysis of 3D tumor spheroid invasion into Matrigel using u-bottom 96-well ULA plates. Examine drug effects on tumor spheroids as a model system for tumor invasion.

Video: Celigo ADCC Assay

ADCC Assay by Direct Cell Counting

Celigo S Image Cytometer

A novel cytotoxicity assay using the Celigo imaging cytometer to directly count live, fluorescently labeled target cells has recently been introduced. Perform qualitative and quantitative analysis for ADCC, CDC, and cell-mediated cytotoxicity assays using a single platform.

Video: Celigo Plate Scan

Celigo Plate Scan

Celigo S Image Cytometer

Proprietary optics and scanning system enable fast imaging of entire well, while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.

Video: Celigo Key Features

Celigo Key Features

Celigo S Image Cytometer

The Celigo instrument has an easy work flow, image auto focus, four color acquisition, imaging & analysis of every cell to the well edge, fast image scanning & analysis, export data & images, and perform population histogram and 2D gating analysis.

Measure Apoptosis Using Caspase 3/7 for Adherent cells

Assay Show 1: Measure Apoptosis Using Caspase 3/7 for Adherent MDA-MB-231 Cells

Celigo S Image Cytometer

This video will show you how to perform an apoptosis assay on the Celigo image cytometer using caspase 3/7 and Hoechst reagents

Video: Apoptosis Suspension

Assay Show 2: Measure Apoptosis Using Caspase 3/7 for Suspension Jurkat Cells

Celigo S Image Cytometer

This video will show an apoptosis assay that was performed using suspension Jurkat cells that were treated with 3 micro molars staurosporine.

Video: Celigo Key Features

Assay Show 3: Kinetic Apoptosis Assay Using Adherent MDA-MB-231 Cells

Celigo S Image Cytometer

This video shows a kinetic apoptosis assay that was performed using an adherent breast cancer cell line MDA-MB-231 that was treated with 3 micromolar staurosporine.

Video: Kinetic Apoptosis Suspension

Assay Show 4: Kinetic Apoptosis Assay Using Caspase 3/7 and Suspension Jurkat Cells

Celigo S Image Cytometer

This video will show you how to perform a kinetic apoptosis assay on the Celigo image cytometer using caspase 3/7 detection reagent and suspension Jurkat cells.

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Vision and Vision CBA Image Cytometry Videos (including cell-based assay demos)

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Quickly and Easily Measure Caspase-8 Activity

Cellometer Vision CBA Analysis System

Activation of Caspase-8 is the first step in the extrinsic apoptosis pathway. Though apoptosis is still reversible at this stage, Caspase-8 activation is frequently used as an early indicator of apoptosis.

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Measure GFP-Positive Cells to Determine Transfection Efficiency

Cellometer Vision CBA Analysis System

With the Cellometer Vision CBA, just 20µl of sample is added to the Cellometer Counting Chamber. Imaging and analysis of GFP expression is completed in less than 60 seconds.

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Image-based Cell Cycle Analysis

Cellometer Vision CBA Analysis System

Perform cell cycle analysis with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain comprehensive plots and tables displaying cell population by phase. Easily switch between users and experiments with no set-up, washing, or potential clogging.

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Image-based Analysis of Apoptosis with Annexin V-FITC / PI

Cellometer Vision CBA Analysis System

Simplify apoptosis experiments with image-based analysis. Colorized data plots easily identify apoptotic and necrotic cells. Manually adjust gating with instant updates to data tables. View bright field and fluorescent images for confirmation of results. Easily switch between users and experiments with no set-up, washing, or potential clogging.

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Assessment of Mitochondrial Membrane Potential using Image-Based Cytometry

Cellometer Vision CBA Analysis System

Perform apoptosis analysis based on changes in mitochondrial membrane potential with only 20µl of sample. See how image-based analysis simplifies cell-based assays. In just a few clicks, acquire cell images and obtain colorized scatter plots and data tables. Easily compare control and experimental samples using a simple, accurate, image-based method.

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Viability of Fragile Hepatocytes using Image-Based Cytometry

Cellometer Vision Analysis System

Primary hepatocytes are a critical component of studies involving liver disease, drug metabolism, and toxicology. Because hepatocytes do not proliferate in culture and display a rapid decline in viability and loss of liver-specific functions under sub-optimal culture conditions, the ability to monitor hepatocytes using a small number of cells is critical. Obtain live/dead cell count, concentration, mean cell diameter and % cell viability for hepatocytes in just 60 seconds from only 20µl of sample using a gentle, non-fluidic analysis method for the most accurate results.

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Measuring Live Cell Concentration and Viability of Human PBMC

Cellometer Vision Analysis System

Cellometer Vision is a line of simple to use, automated cell analyzers with diverse functions. It combines bright field microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. This video demonstration features the Cellometer Vision to measure live cell concentration and viability of human PBMC.

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Yeast Cell Concentration & Viability in Corn Mash

Cellometer Vision Analysis System

Cellometer Vision is a line of simple to use, automated cell analyzers with diverse functions. It combines bright field microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. This video demonstration features the Cellometer Vision to measure yeast cell concentration and viability in a highly viscous corn mash sample.

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Cellometer K2 and X2 / X1 Videos

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The Cellometer K2 Image Cytometer for Cell Counting and Analysis

Cellometer K2 Image Cytometer

This video shows an example of a viability measurement performed with the Cellometer K2. 20 micro liters of PMBC sample is mixed with Acridine Orange and Propidium Iodide solution and then loaded into the Cellometer counting chamber.

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The Cellometer X2 Image Cytometer for Cell Counting and Analysis

Cellometer X2 / X1 Image Cytometer

This video shows an example of a viability measurement performed with the Cellometer X2. 20 micro liters of yeast sample is mixed with Propidium Iodide solution and then loaded into the Cellometer counting chamber and analyzed.

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1-step Yeast Concentration & Viability Determination

Automatically generate yeast concentration and viability data from a single sample. See how bright field and fluorescent imaging simplifies and enhances yeast fermentation monitoring with 1-click image capture and calculation of yeast concentration, % viability, and cell size. Save time and improve data quality and reproducibility vs. manual counting.

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Cellometer Auto X4: Measuring Algae Cell Concentration

Cellometer Auto X4 is a line of simple to use, automated cell counters with fluorescence detection. It combines bright field microscopy with fluorescence to identify selected cells. This video demonstration features the Cellometer Auto X4 to measure algae cell concentration from a pond water sample.

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Cellometer Auto 2000 Videos

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Cellometer Auto 2000 Cell Analyzer Demonstration

Cellometer Auto 2000 Cell Counter

This demonstration of the Cellometer Auto 2000 operation shows how quickly the automated system delivers total cell count, concentration and mean diameter from only 20µl of sample. Results also include a cell size histogram and percent viability.

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Overview of Cellometer Auto 2000 Assays

Cellometer Auto 2000 Cell Counter

Perform two-color fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. Accurately measure PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using sample-specific assays and a simple touch screen.

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Primary Cell Concentration & Viability using Cellometer Auto 2000 Cell Viability Counter

Cellometer Auto 2000 Cell Counter

Perform two-color fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. Accurately measure PBMCs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using sample-specific assays and a simple touch screen.

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Cellometer Auto T4, Auto 1000 and Mini Videos

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Cellometer Mini Cell Counter Demo

Cellometer Mini Cell Viability Counter

Demonstration of Cellometer Mini, the most affordable PC-based, automated 4x bright field for cell count, size, and Trypan Blue viability measurements of cell lines and purified primary cells.

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Cellometer Auto 1000 Cell Viability Counter Demo

Cellometer Auto 1000 Cell Viability Counter

Demonstration of Cellometer Auto 1000, the all-in-one, touch-screen instrument for automated 4x bright field analysis of cell count, size, and Trypan Blue viability in cell lines and purified primary cells.

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Improve Cell Counting Speed and Accuracy

Cellometer Auto T4 Cell Counter

Accurate cell concentration and viability calculations are critical for successful cell-based assays and flow cytometry analysis. The accuracy and reproducibility of the Cellometer Auto T4 Cell Counter have been demonstrated in thousands of labs, including the top ten pharmaceutical companies.

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Cellometer Assay Principle Videos

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Dual-fluorescent AO/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent AO/PI assay for cell concentration and viability. This assay is most suitable for primary cell samples which contain debris and unwanted non-nucleated cell types.

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Dual-fluorescent AO/EB Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent AO/EB assay for cell concentration and viability. This assay is most suitable for primary cell samples such as white blood cells, splenocytes and lymphocytes which contain debris and unwanted non nucleated cell types such as red blood cells.

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Dual-fluorescent Calcein AM/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent Calcein AM/PI assay for cell concentration and viability Calcine AM penetrates the cell membrane of both living and dead cells. Once inside a living cell, Calcine AM is hydrolyzed by cellular esterases into the cell membrane-impermeable Calcein. PI is only permeable to dead cells.

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Dual-fluorescent Fluorescein Diacetate (FDA)/PI Assay for Cell Concentration and Viability

Cellometer Assay Principal

A Cellometer assay principal illustration of a dual-fluorescent FDA/PI assay for cell concentration and viability.

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Cellometer Software Feature Videos

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Save and Export Data

Cellometer Features

Keeping records of your research is critical. With the click of a button, Cellometer instantly saves counting results, cell images, graphs and data to a clipboard, local or network location.

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Sample Adjustment Calculator

Cellometer Features

The Cellometer Sample Adjustment Calculator is a unique feature that saves time and reduces human error. This tool automatically calculates the number of cells in the sample and allows you to change the original sample volume to meet desired target cell concentration.

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Cell Size Measurement

Cellometer Features

Unlike manual counting, Cellometer measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.

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Import Cell Type

Cellometer Features

The Cellometer import cell type feature uses the Nexcelom Cell Library that contains optimized counting parameters for more than 300 difference cell types. Users can import any selected cell type by browsing the Nexcelom Cell Library. Cellometer application specialist will optimize counting parameters for your cell type, free of charge.

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Cell Type Wizard

Cellometer Features

Cell type wizard is designed to automatically set up cell counting parameters for any new cell lines.

Informational Webinars On Demand

Play Video

How to Measure Viability of Adipocytes

Cellometer

Are you a diabetes researcher, liposuction researcher, stem cell researcher or just interested in how image cytometery can be used to analyze adipocytes? Are you interested in learning the benefits of image cytometry? In this informational webinar, Dr. Dmitry Kuksin of Nexcelom will explore these topics and more.

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High Throughput Analysis for Viral Infection Assays

Celigo

In this informational webinar, Dr. Dmitry Kuksin, Nexcelom Senior Scientist examines specific virology related assays such as plaque and foci formation, as well as antibody neutralization. Additionally, this webinar will focus on how these assays are curently being conducted and the benefits of the Celigo image cytometer.

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AOPI vs. Trypan Blue: Differences Between These Two Methods for Cell Viability Measurement

All Cellometer

Are you using trypan blue for cell counting and viability? Are you interested in learning about the benefits of fluorescent staining methods?  In this informational webinar, Dr. Leo Chan of Nexcelom will explore these topics and more. 

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Improving Cell Counting and Viability Detection with a High-Throughput Method

Celigo

In this informational webinar, Nexcelom's Director of Applications and Emerging Technologies, Dr. Scott Cribbes, takes a look cell counting and viability assays. Key take aways include: A history of cell counting and viability. Review of current methods to count cells and detect viability. How image cytometry can be used for high-throughput cell counting and viability detection. Case Study: Dual fluorescent method for use with cell line development.

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Importance of Accuracy and Consistency for Viability and Concentration in the field of Adoptive Cell Transfer Therapy

All Cellometer

Are you performing immuno-oncology assays in the field of adoptive cell transfer therapy? Are you curious to learn about current methods used by other researchers, and see how an automated cell counter could benefit your work? In this informational webinar, Dr. Leo Chan of Nexcelom will explore these topics and more. Key take-aways for this webinar include: What is adoptive cell transfer therapy? What cells are commonly used in ACT? What are the counting and viability methods for these cell types? Where does the Cellometer automated cell counter fit into the workflow? Advanced assays showing direct NK cell-mediated cytotoxicity for in vitro functional assays.

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How to Perform High Throughput Cell Cycle Assays

Celigo

In this informational webinar, Nexcelom's Director of Applications and Emerging Technologies, Dr. Scott Cribbes, takes a look cell cycle. How is it currently examined? Is there a better way? What if cell cycle assays could be performed in a high throughput method? How might the Celigo image cytometer provide an alternative solution to biologists and researchers?

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2D and 3D Kinetic Apoptosis Assays for Cancer Research

Celigo

In this informational webinar, Dr. Scott Cribbes, Director of Applications and Emerging Technologies, takes a look at 2D and 3D apoptosis. Dr. Cribbes starts off with a joke about weight loss due to the apoptotic cells in your body before looking at what is apoptosis, what is the timeline and what are the mechanisms. He then will examine the current methods to perform apoptosis assays, before comparing using flow cytometry to image cytometry to review advantages and drawbacks of each method. Dr. Cribbes concludes by introducing the Celigo image cytometer as a solution for your 2D and 3D assays.

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How to Improve your Cell Cycle Analysis

Cellometer K2, Vision and Vision CBA

In this informational webinar, Assay Development Scientist, Dr. Dmitry Kuksin explores cell cycle analysis. Dr. Kuksin begins by looking at the importance of cell cycle research and detailing the basic biology of cell cycle and reviews a typical protocol for performing cell cycle. Dr. Kuksin then describes how cell cycle can easily be performed on a Cellometer image cytometry system and also how it compares to results that would be generated if it was performed on a flow cytometer.

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3D Organoid Models for Cancer Research

Celigo

In this informational webinar, Dr. Dmitry Kuksin, Assay Development Scientist, explores patient-derived organoids or PDOs. Dr. Kuksin starts be discussing what is a PDO and where do they come from, as well as how are they being used in cancer research. He then explores the advantages and challenges of using PDOs, as well as the challenges that currently exist to image and analyze PDOs - as they are often grown in Matrigel. Dr. Kuksin concludes by introducing the Celigo image cytometer as a solution to image and analyze PDOs.

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Improving your Primary Cell Analysis: Make it Accurate, Quick and Simple

Cellometer Auto 2000, K2, Vision and Vision CBA

In this informational webinar, Product Manager, Timothy Smith, takes a look at primary cell analysis. Primary cell samples such as PBMCs, bone marrow and cord blood, can often be difficult to count with traditional methods of manual counting with Trypan Blue staining. Tim examines these challenges, and presents an alternative with fluorescent counting using the Cellometer Auto 2000 and AO/PI viability staining. He reviews the instrument's capability and shares how one of our current users has incorporated the instrument into their workflow.

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Challenges and Solutions of Performing High-Throughput 2D Migration/Invasion Assays

Celigo

In this informational webinar, Dr. Dmitry Kuksin, Assay Development Scientist, examines high-throughput 2D migration and invasion assays. Dr. Kuksin begins by reviewing common methods such as Boyden, scratch assays, exclusion zone, etc. He then looks in great detail the various advantages and drawbacks for these current, common methods. From there, Dr. Kuksin presents the Celigo image cytometer as an alternative solution for wound healing assays and provides an example of using a direct cell counting method for a chemotaxis assay

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Can you Trust Automated Cell Counting?

All Cellometer

In this informational webinar, Product Manager, Timothy Smith, examines a commonly asked question - can automated cell counting be trusted? Tim covers aspects such as the instrument's accuracy, consistency and repeatability as means of building trust and confidence in the results. He also examines current challenges with manual counting methods. To wrap up this webinar, Tim reviews how to validate and perform verification on an automated system once you've brought one into your lab.

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Improving ADCC Assays using Image Cytometry

Celigo

In this informational webinar, Dr. Leo Chan, R&D Manager at Nexcelom Bioscience explores current, conventional methods to perform ADCC assays and explores image cytometry as an alternative, improved method. Dr. Chan begins by reviewing the current methods and provides some advantages of using image cytometry. Lastly, Leo provides real examples of assays performed with image cytometry including direct tumor cytotoxicity, direct cell-mediated cytotoxicity, ADCC and CDC.

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Is Automated Cell Counting Right For You?

Cellometer

In this informational webinar, Timothy Smith, Cellometer Product Manager at Nexcelom Bioscience takes a look at what labs should consider to help determine if automated cell counting is right for them. Tim begins by reviewing a sample list of 10 questions to ask before purchasing an automated cell counter and then reviews challenges presented by the golden standard method of manual counting with a hemacytometer. Tim then looks at various automated instruments available, compares them for cost, required sample volume needed, time per test and the type of data each produces. Finally, Tim examines the Cellometer systems against those same questions and important requirements, to see how the Cellometer automated cell counters stack up to the competition.

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3D Tumor Spheroids-Based Functional Assays on the Celigo Image Cytometer

Celigo

In this technical informational webinar, Dr. Olivier Dery, Director of Celigo Business at Nexcelom Bioscience will review a series of topics pertaining to 3D Tumor spheroid models. He starts with a quick overview of the Celigo image cytometer, an instrument provided by Nexcelom, that provides high throughput full well imaging and analysis with bright field and 4 channels of fluorescence, on adherent and suspension cells. From there, the rest of the webinar covers many aspects to 3D Tumor Models. 3D models have been demonstrated to be more predictive of effective clinical therapies and more reflective of tumor microenvironments. We then explore benefits and disadvantages of various methods to generate 3D tumor spheroids including: hanging drops, scaffolding, matrigel coated and ULA plates. We explore spheroid nomenclature - reviewing the types of spheres that can be formed: tight spheroids, compact aggregates and loose aggregates; and review cell types we've tested and which type of spheroid we are able to create with them. We take the time to review some key applications in 3D models that can be performed using the Celigo, with examples of work done on the Celigo by one of our customers (Vinci et al., BMC Biology 2012 / Vinci et al., Methods in Mol Biol 2013 / Vinci et al., JoVE 2015). In this section, we look at the experimental set up for each assay and also present actual data and images collected from using the Celigo. Experiments covered include: growth inhibition assays, migration assays with ECM, invasion into matrigel. Lastly, we look at how the Celigo can perform viability assays and drug combination treatment screening experiments on 3D tumor spheroids.

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3D Multicellular Tumor Spheres: How to get Started

Celigo

In this informational methods webinar, Dr. Scott Cribbes, Director of Applications and Emerging Markets at Nexcelom Bioscience will take a look at 3D models. It is a hot topic and he will explore why so many researchers are interested in working in 3D models. (Teaser: MCTS assays are more clinically relevant and predictive models than 2D assays) This presentation covers everything you need consider and evaluate if you're considering working in 3D models. Dr. Cribbes reviews and explore the confusing terminology out there surrounding 3D models - multicellular tumor spheroids (MCTS); tumorspheres (TS); tissue-derived tumor spheres (TDST); organotypic multicellular spheroids (OMS) - how are these different? Similar? Dr. Cribbes move on to explain the MCTS model and the current methods to generate 3D spheroids. There's discussion of considerations that need to be made prior to moving to a 3D model, to help ensure the greatest potential for success, and also examine ultra-low attachment u-bottom plates and discuss the significant advantages of using these when working with 3D MCTS. Finally, he'll review a few assays popular for MCTS including: drug effect assays (growth inhibition, viability and apoptosis screens). Lastly, Dr. Cribbes also speaks about the Celigo image cytometer as a solution for imaging and analysis of 3D MCTS kinetic and endpoint assays.

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Challenges Facing Immuno-Oncology Researchers with High-Throughput Cell Counting

Celigo

In this informational webinar, Dr. Dmitry Kuksin, Assay Development Scientist at Nexcelom Bioscience explores options for researchers to perform high-throughput cell counting through assays such as MTT, Cell-Titer Glo and manually counting. Each method is evaluated for advantages and disadvantages. Dr. Kuksin then discusses the importance of high-throughput cell counting for immuno-oncology researchers studying drug effects on cancer cells before presenting an alternative method with the Celigo imaging cytometer. Finally, highlights of researchers using the Celigo are presented.

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Rapid yeast viability detection method in complex brewing samples using the Cellometer X2 Image Cytometry

Cellometer X2

In this technical informational webinar, Dr. Leo Chan, R&D Manager at Nexcelom Bioscience will review yeast viability, and specifically address challenges that arise when working with complex or messy samples. This session was developed and presented with breweries and brewers in mind, to help discuss various solutions. He starts by looking at why it is important to determine yeast viability for fermentation and differentiates between simple and complex samples. Then Dr. Chan reviews various detection methods - both traditional methods and the Cellometer X2 image cytometry method, to see advantages/disadvantages of each. From there, the Acridine Orange and Propidium Iodide yeast viability method is explained, reviewed and discussed, before covering validation of the viability detection methods.

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Quick and Easy Way to Measure Yeast Concentration and Viability During Beer Fermentation

Cellometer X1/X2

In this technical informational webinar, Dr. Leo Chan, R&D Manager at Nexcelom Bioscience presents information specifically developed and presented with breweries and brewers in mind. Dr. Chan begins by presenting a brief introduction to Nexcelom, and also some information about image cytometry and the Cellometer systems. Then he reviews various detection methods - both traditional methods and the Cellometer X1 and X2 image cytometry methods, to see advantages/disadvantages of each. Dr. Chan also addresses how the Cellometer X2 can perform vitality assays on yeast samples before wrapping up the seminar by recapping how to determine if the Cellometer X1 or Cellometer X2 is the best solution for you.

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Isolating Primary Cells 101: Which viability method is best for your research?

Cellometer

In this webinar, AllCells and Nexcelom join together to answer some important questions, such as: What is the process for obtaining cells from bone marrow? What is the process for obtaining cells from peripheral blood? What is the best and most accurate cell counting and viability method for primary samples? How do we select the appropriate method for viability measurement? James Lee of AllCells kicks our session off, to discuss how they are able to provide a superior cell product, and then Dr. Leo Chan reviews different viability assays, and discusses how to find the best viability method for your research, based on your cells.

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