Nexcelom’s CTO on the Future of CAR T Workflow
Nexcelom founder and CTO, Dr. Jean Qiu, describes 3 major reasons why we partner with global and local research organizations to streamline CAR-T workflow.
Advantages of a Standardized Automated Cell Counting Method for Cell Therapy
Q & A regarding the crucial need for accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.
Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay
Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.
Video Workflow-at-a-Glance: Seahorse™ Normalization Assay
Celigo Image Cytometer provides a fast, effective, and cell stress-free technique to count cells plated onto uncoated or coated culture plates.
Normalizing Seahorse™ XF Data with Label-Free Cell Counting
How to use Celigo image cytometry to normalize Seahorse™ XF data and eliminate time-consuming protein-based process.
Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision Automated cell counting methods can monitor yeast concentration and viability throughout fermentation to ascertain cell health and the amount of yeast to be pitched or repitched, all of which contributes to the quality and flavor consistency of the final product [1,2,3]. Analyzing physiological and metabolic characteristics of the yeast cells permits operators to efficiently monitor yeast viability and vitality for quality control purposes, which impacts long-term storage and other physiological stresses. Download our white [...]
A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy. Download our white [...]
Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation
Over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools
Using the Cellometer Vision Image Cytometer for Immunophenotyping
It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]
Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer
It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »