About Leo Chan

Dr Leo Chan currently serves as the Technology R&D Manager at Nexcelom Bioscience LLC, Lawrence, MA. His research involves in the development of instrument and applications for the Celigo and Cellometer image cytometry system for detection and analysis of cells for oncology and immunology research. He is a member of the American Association of Cancer Research and the American Association of Immunologists. He received his B.S., M.S., and Ph.D. in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign (2000-2008).

Is your 2D assay giving superficial information?

Perform simultaneous imaging and analysis to rapidly capture and quantify spheroid images in every well with advanced software segmentation.

Cold-chain Storage Optimization for Umbilical Cord Human Mesenchymal Stem Cells

Effective long-term cryopreservation of human mesenchymal stem cells is an essential component of regenerative medicine and tissue engineering applications.

Improving the Gene Therapy Development Process using Image Cytometry

Many factors and considerations must be optimized to develop the appropriate cell-based assays for gene therapy development.

How to count cells using a hemocytometer

View these simple step-by-step instructions for counting cells using a hemoctyometer and microscope. Includes the formula for calculating the dilution factor.

Is your trypan blue rupturing dead or dying immune cells leading to an over-estimation of cell viability?

It is important to select the most accurate cell counting method that is fit-for-purpose where cell number and viability significantly affect treatment efficacy.

Identify & Resolve Manual Cell Counting Errors through Automation

The use of modern automation has largely eliminated many of the hemocytometer's sources of error, increasing the accuracy and efficiency of cell counting today.

Nexcelom’s CTO on the Future of CAR T Workflow

Nexcelom founder and CTO, Dr. Jean Qiu, describes 3 major reasons why we partner with global and local research organizations to streamline CAR-T workflow.

Advantages of a Standardized Automated Cell Counting Method for Cell Therapy

Q & A regarding the crucial need for accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.

Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay

Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.

Video Workflow-at-a-Glance: Seahorse™ Normalization Assay

Celigo Image Cytometer provides a fast, effective, and cell stress-free technique to count cells plated onto uncoated or coated culture plates.

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