About Leo Chan

Dr Leo Chan currently serves as the Technology R&D Manager at Nexcelom Bioscience LLC, Lawrence, MA. His research involves in the development of instrument and applications for the Celigo and Cellometer image cytometry system for detection and analysis of cells for oncology and immunology research. He is a member of the American Association of Cancer Research and the American Association of Immunologists. He received his B.S., M.S., and Ph.D. in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign (2000-2008).

Poster: Celigo Imaging Cytometry for Cell Permeability

Download the Poster Development of an image-based HCS compatible method for endothelial barrier function assessment Oleksii Dubrovskyi, Erica Hasten, Steven M. Dudek, Michael T. Flavin, and Leo Li-Ying Chan Acute respiratory distress syndrome (ARDS) is a serious condition with high mortality rate that has increased in the recent years due to the COVID-19 pandemic. Currently, effective pharmacological therapies have yet been discovered for ARDS, despite decades of laboratory and clinical studies. ARDS onset typically generates an increase in the endothelial permeability causing the development of pulmonary edema that leads to respiratory failure during the primary event. In this work, we [...]

By |2022-06-13T15:58:15+00:00March 8th, 2022|0 Comments

Poster: Instrument to Instrument Consistency for Cell Therapy Applications

Download the Poster Large-Batch Evaluation of Cell Counter Instrument-to-Instrument Consistency for Cell Therapy Applications Jordan Bell, Yongyang Huang, Cecelia Williamson, Jacob Chartrain, Sun Yung, Suzanne Riches and Leo Li-Ying Chan Cell therapy has been heralded as a medical revolution, and its rise has only increased the importance of accuracy and precision in cell counting. Ensuring the identity, purity, concentration, and viability of cell therapy products is critical for increasing efficacy and avoiding potential harmfulside effects. A significant portion of this burden now rests on sample analysis by automated cell counters. As cell therapies move from experimentation to production, the need [...]

By |2022-06-13T15:58:44+00:00March 8th, 2022|0 Comments

Is your 2D assay giving superficial information?

Perform simultaneous imaging and analysis to rapidly capture and quantify spheroid images in every well with advanced software segmentation.

Poster: Apoptosis Analysis of Jurkat Cells using Cellometer Vision

Download the Poster Apoptosis Analysis of Jurkat Cells using the Cellometer Vision Leo L. Chan, Tim Smith, Alnoor Pirani, Emily Lyettefi, Ning Lai, Jean Qiu, and Bo Lin We demonstrate a rapid and cost-effective method for apoptosis analysis at various stages of Jurkat cells using the Cellometer® Vision. This method has the ability to eliminate many known issues caused by manual hemacytometer and flow cytometer. By using Cellometer® Vision, the assay time for obtaining apoptosis result is greatly reduced, which is significant for research development in academia and industry. The current methods for apoptosis analysis utilizes standard flow cytometry, but [...]

By |2022-06-13T16:00:51+00:00March 31st, 2021|0 Comments

Poster: A Novel Imaging Cytometry Method for Immunophenotyping

Download the Poster A Novel Imaging Cytometry Method for Immunophenotyping Leo L. Chan, Xuemei Zhong, Jean Qiu, Peter Y. Li, Bo Lin The ability to detect and enumerate cells with specific surface markers is essential for clinical diagnosis and biomedical research. The traditional cell analysis instrumentation for immunophenotyping involves the use of fluorescence microscopy, confocal fluorescence scanning, and flow cytometry. However, these systems are often expensive to purchaseand maintain. In this work, we demonstrate a novel low-cost device, Cellometer® system, which combines both microscopic imaging and cytometry methods with comparable detection sensitivity. This method utilizes both bright-field and fluorescence imaging [...]

By |2022-06-13T16:00:51+00:00March 31st, 2021|0 Comments

Poster: A Rapid Alternative Method for Cell Cycle Analysis Using Cellometer Vision

Download the Poster A Rapid Alternative Method for Cell Cycle Analysis Using Cellometer Vision Leo L. Chan, Xuemei Zhong, Jean Qiu, Peter Y. Li, and Bo Lin Traditional cell cycle analysis instrumentation involves the use of fluorescence microscopy, laser scanning cytometry, and flow cytometry. In this work, we demonstrate new applications for the Cellometer Vision, which utilizes an imaging cytometry method for cell cycle analysis. This method employs both bright-field and fluorescence imaging of a disposable cell counting chamber to quickly provide concentration and percentages of cell subpopulations. Signal measurement and data analysis could be performed less than fivemin. Cell [...]

By |2022-06-13T16:00:57+00:00March 31st, 2021|0 Comments

Cold-chain Storage Optimization for Umbilical Cord Human Mesenchymal Stem Cells

Effective long-term cryopreservation of human mesenchymal stem cells is an essential component of regenerative medicine and tissue engineering applications.

Improving the Gene Therapy Development Process using Image Cytometry

Many factors and considerations must be optimized to develop the appropriate cell-based assays for gene therapy development.

How to count cells using a hemocytometer

View these simple step-by-step instructions for counting cells using a hemoctyometer and microscope. Includes the formula for calculating the dilution factor.

Is your trypan blue rupturing dead or dying immune cells leading to an over-estimation of cell viability?

It is important to select the most accurate cell counting method that is fit-for-purpose where cell number and viability significantly affect treatment efficacy.

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