/Leo Chan
Leo Chan

About Leo Chan

Dr Leo Chan currently serves as the Technology R&D Manager at Nexcelom Bioscience LLC, Lawrence, MA. His research involves in the development of instrument and applications for the Celigo and Cellometer image cytometry system for detection and analysis of cells for oncology and immunology research. He is a member of the American Association of Cancer Research and the American Association of Immunologists. He received his B.S., M.S., and Ph.D. in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign (2000-2008).

Identify & Resolve Manual Cell Counting Errors through Automation

The use of modern automation has largely eliminated many of the hemocytometer's sources of error, increasing the accuracy and efficiency of cell counting today.

Nexcelom’s CTO on the Future of CAR T Workflow

Nexcelom founder and CTO, Dr. Jean Qiu, describes 3 major reasons why we partner with global and local research organizations to streamline CAR-T workflow.

By |2019-01-15T20:49:37+00:00January 15th, 2019|Categories: Accurate Cell Counting, Latest News|Tags: , , , , |0 Comments

Advantages of a Standardized Automated Cell Counting Method for Cell Therapy

Q & A regarding the crucial need for accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.

By |2018-12-13T19:46:26+00:00December 13th, 2018|Categories: Accurate Cell Counting, Latest News|Tags: , |0 Comments

Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay

Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.

Video Workflow-at-a-Glance: Seahorse™ Normalization Assay

Celigo Image Cytometer provides a fast, effective, and cell stress-free technique to count cells plated onto uncoated or coated culture plates.

Normalizing Seahorse™ XF Data with Label-Free Cell Counting

How to use Celigo image cytometry to normalize Seahorse™ XF data and eliminate time-consuming protein-based process.

By |2018-11-20T22:36:09+00:00November 20th, 2018|Categories: Celigo Application News, Latest News|Tags: , , , |0 Comments

Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision Automated cell counting methods can monitor yeast concentration and viability throughout fermentation to ascertain cell health and the amount of yeast to be pitched or repitched, all of which contributes to the quality and flavor consistency of the final product [1,2,3]. Analyzing physiological and metabolic characteristics of the yeast cells permits operators to efficiently monitor yeast viability and vitality for quality control purposes, which impacts long-term storage and other physiological stresses.     Download our white [...]

A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy.     Download our white [...]

By |2019-10-25T00:53:34+00:00March 2nd, 2016|Categories: Cellometer Application News|Tags: , , |0 Comments

Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By |2015-10-21T10:30:34+00:00October 21st, 2015|Categories: Cellometer Application News|Tags: , , , , , , |0 Comments