Leo Chan, Author at Nexcelom Bioscience

Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision Automated cell counting methods can monitor yeast concentration and viability throughout fermentation to ascertain cell health and the amount of yeast to be pitched or repitched, all of which contributes to the quality and flavor consistency of the final product [1,2,3]. Analyzing physiological and metabolic characteristics of the yeast cells permits operators to efficiently monitor yeast viability and vitality for quality control purposes, which impacts long-term storage and other physiological stresses. Download our white [...]

By Leo Chan|2016-03-09T10:00:07+00:00March 9th, 2016|Categories: Cellometer Application News|Tags: Breweries, Cellometer Vision, Yeast Fermentation, yeast viability, Yeast Vitality|0 Comments

A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy. Download our white [...]

By Leo Chan|2016-03-02T10:00:54+00:00March 2nd, 2016|Categories: Cellometer Application News|Tags: Cellometer Vision, Cytotoxicity, Naturan Killer Cells|0 Comments

Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation These studies reveal that over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools, although the studies did not speculate as to the reasoning behind the differences noted (Jones and Senft 1985; Mascotti et al. 2000). In this work, we perform a head-to-head comparison between cell viability as determined by TB and that determined by AO/PI using an automated image-based cytometer, the Cellometer. Image-based cytometry allowed us to examine the [...]

By Leo Chan|2016-02-24T10:00:48+00:00February 24th, 2016|Categories: Cellometer Application News|Tags: Cellometer Vision, Morphological Analysis, Trypan Blue vs AOPI, Viability Staining|0 Comments

Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By Leo Chan|2015-10-21T10:30:34+00:00October 21st, 2015|Categories: Cellometer Application News|Tags: B220, CD4, CD5, CD8, immunophenotyping, Surface marker, White Paper|0 Comments

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By Leo Chan|2015-10-07T10:30:08+00:00October 7th, 2015|Categories: Cellometer Application News|Tags: Aphidicolin, Cell Cycle, Etoposide, Nocodazole, White Paper|0 Comments

Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »

By Leo Chan|2015-09-23T10:30:58+00:00September 23rd, 2015|Categories: Cellometer Application News|Tags: Apoptosis, CCCP, Mitochondrial Potential, Necrosis, White Paper|0 Comments

Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer In this study, using the CHO cell line, the Celígo Imaging Cytometer was used to monitor transfection efficiency, identify passaging times, optimize media, identify single cells and track clone outgrowth, following previous studies detailing Celígo's performance in tracking cell lineage and confluence [3, 4]. After transfection, cells were allowed to stabilize, then imaged in bright field and fluorescence to monitor and evaluate transfection efficiencies. As wells grew asynchronously, bright field imaging was used to track which wells needed passaging. As [...]

By Leo Chan|2015-09-09T10:30:42+00:00September 9th, 2015|Categories: Celigo Application News|Tags: Cell Line Development, White Paper|0 Comments

Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements [12]. Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]

By Leo Chan|2015-08-19T10:30:23+00:00August 19th, 2015|Categories: Cellometer Application News|Tags: Stromal Vascular Fraction (SVF), Viability, White Paper|0 Comments

A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]

By Leo Chan|2015-08-05T10:30:08+00:00August 5th, 2015|Categories: Celigo Application News|Tags: Cell Proliferation, Drug toxicity, Label-free, White Paper|0 Comments

Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]

By Leo Chan|2018-06-20T20:32:04+00:00July 22nd, 2015|Categories: Cellometer Application News|Tags: B Cells, B1, B2, Cell Proliferation, White Paper|0 Comments

About Leo Chan