Cellometer Fluorescent Cell Counters for Mouse Samples
Obtain fast, accurate and consistent cell viability and concentration measurements of primary murine samples in <30 seconds using the Cellometer K2!
Perform quick & easy cell based assays using Cellometer Image Cytometer
Using Cellometer Image Cytometer to Perform Consistent and Accurate Cell Cycle, Apoptosis, and GFP Analysis Cellometer K2 and Vision instruments are capable of not only accurately and quickly measuring the cell concentration and viability, but also have the capability to perform multiple cell-based assays. Below we highlight three such assays: Cell Cycle Apoptosis GFP Performing Cell Cycle Assay Analyze and compare the effects of different cell cycle pharmacological agents. Provides researchers with the ability to visually review and confirm counted cells. Use the provided FCS Express™ templates for quick and easy data analysis and presentation. Data acquired on the Cellometer [...]
Impact of RBC contamination in clinical samples
Bone marrow, cord blood, whole blood, and peripheral blood are routinely processed in many different laboratories. Whether for cryopreservation or for downstream isolation of specific nucleated cells, (such as stem cells, B-cells, or T-cells) accurately measuring cell concentration and viability is paramount to the overall success of the project. Many blood-based samples (whole blood, peripheral blood, bone marrow, PBMC, cord blood, etc…) may contain residual red blood cells even after RBC lysis. (See figure below) When samples are enumerated using manual counting, the presence of residual RBCs can lead to inaccurate cell concentration and viability readings. Nucleic acid dyes, acridine [...]
Live Webinar: Isolating Primary Cells 101: Which Viability Method is Best for Your Research?
Cellometer Auto 2000 Cell Viability Counter Thursday, February 13, 12-12:30 PM EST Presented by: Leo Chan, Nexcelom Bioscience Technology R&D Manager James Lee, AllCells Laboratory Manager What process is used to obtain cells from bone marrow and normal peripheral blood? What is the best cell counting and viability method for primary cells? Nexcelom Bioscience, your cell counting experts, and AllCells, your primary cells research partner, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences. Join our Laboratory Scientists for this collaboration including a Q&A [...]
Video Presentation: Using Cellometer Image Cytometry to Detect and Measure GFP Expression Efficiency
This presentation will focus on using Cellometer image cytometry to effectively determine GFP or other fluorescent protein transduction/transfection efficiency and cell concentration, as well as detect and quantify dual expression in a cell population. Quantifying GFP can be done in four easy steps. First pipette 20 ul of target cell sample into a disposable counting chamber. Then insert the slide into the instrument, select your assay from the drop-down menu, focus and click count. Within seconds the instrument acquires images, identifies cells with and without fluorescence and automatically tabulates the results. The results include the number of cells counted in [...]
Nexcelom has performed Cellometer image cytometry on all NCI-60 cancer cell lines
Nexcelom has performed Cellometer image cytometry analysis on all NCI-60 cancer cell lines.
Automate Hemocytometer for Murine Primary Samples
Cellometer Bright Field and Fluorescent Cell Counters for Murine Primary Samples Obtain fast, accurate, and consistent cell viability and concentration measurements of primary murine samples in < 30 seconds — Tissue debris, cell debris, and red-blood cell contamination can lead to inconsistent cell counts from sample to sample when performing manual counting Performing fluorescent-based analysis using Acridine Orange/ Propidium Iodide (AO/PI) allows for the identification and enumeration of only nucleated cells Cell concentration and viability are two common measurements that are performed for great variety of primary murine samples: Mouse Tail Vein Bleeds, Splenocytes, Lymphocytes, BAL, Tissue Digests, Tumor Digests, [...]
Trypan Blue vs. Acridine Orange/Propidium Iodide to measure cell viability
Do you have red-blood cells, cell debris, or tissue debris in your samples? When your samples contain unwanted particles use: Acridine Orange/ Propidium Iodide (AO/PI) viability assay to accurately measure the concentration and viability of your samples! AO/PI Protocol Obtain Nexcelom AO/PI solution: Cat # CS2-0106-5ML Stain cell sample at 1:1 with AO/PI solution Load counting chamber slide with 20 µl of cells and analyze Report Accurate and Consistent concentration and viability Results Do you have clean cell samples without RBCs, or other cell debris? When your samples are free from unwanted particles, Use Trypan Blue exclusion assay to accurately [...]
AllCells Partners with Nexcelom using Cellometer Technology
AllCells is excited to announce our collaboration with Nexcelom, the clear leader and innovator in the field of image-based cytometry for cell analysis. At AllCells,we understand the importance of continually updating our research instruments with the most innovative in the field in order to provide our customers with the highest quality and technologically advanced products and services. Late last year, we began using Nexcelom’s Cellometer technology to more accurately count our cells in lab. […]
Using Image Cytometry for the Detection and Analysis of GFP Expression
Perform image-based GFP analysis WITHOUT all of the set-up, maintenance, and data manipulation required for flow. Measure GFP Expression Efficiency Using Image Cytometry — In just a few clicks, Cellometer Vision CBA generates brightfield and fluorescent cell images, and detailed data reports. Detection of GFP expression to quantify the transfection/transduction efficiency. Single assay for measuring GFP expression and viability Measurement of other fluorescent proteins Detection of GFP Expression to Quantify the Transfection/Transduction Efficiency Quantitative measurement of GFP expression using Cellometer Load only 20 µl sample into counting chamber Capture and analyze brightfield and fluorescent cell images automatically Data [...]