Nexcelom Bioscience

Cellometer Cell Counting and Image Cytometry Applications

Nexcelom offers a range of automated cell counters and image cytometers coupled with state of the art image analysis software to address a variety of cell types and applications from basic cell counting and viability to fluorescence cell-based assays. With over 500 peer-reviewed studies published by Cellometer and Celigo users and over 1,600 different cell lines verified on the equipment, we have the experience necessary to solve your cytometry needs. We actively partner with our customers and collaborate with academia and industry professionals to develop novel assays, produce new publications, and create original conference presentations in areas such as oncology, immunology, regenerative medicine, environmental biology, brewing yeast fermentation, and renewable energy including biofuels. Browse through our application gallery to see how Cellometer can be put to use in your lab. For more applications, please visit our user publication page.



Adipocytes

Adipocytes

Automatically measure cell size of freshly isolated adipocytes and plot size histogram. DNA staining fluorescence dyes are used to identify cells from lipid droplets. »

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Apoptosis

Apoptosis

Automatically detect and analyze Caspase3 and 8, JC-1, and Annexin V apoptotic events using the Cellometer image cytometery. »

Apoptosis

Image Cytometry for Surface Marker and Intracellular Protein Detection

Use the Cellometer Vision CBA to quickly and easily measure and analyze cell populations based on surface and intracellular proteins expressions. »

Image cytometry for autophagy detection

Image Cytometry for Autophagy Detection

Analyze autophagy activity in living cells using CytoID® Green dye and GFP-labeled LC3 marker. Fluorescence histograms are used to measure mean fluorescence intensity of live cells, and then used to determine the autophagy activity factors in the population. »

Cell Cycle

Image Cytometry for Cell Cycle Analysis

Automatically measure the cell cycle of mammalian cells. Generated cell cycle histogram allows for easy data analysis and presentation. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Viability and Necrosis

Cell Viability and Necrosis

Cell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »

Blood Samples

TNC Concentration & Viability for Clinical (Blood) Samples

Analyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

GFP Transfection Efficiency

Quantitative Measurement of GFP Transfection

Rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection automatically. »

Hepatocytes

Fresh & Cryo Preserved Primary Hepatocytes

Automatically measure live hepatocyte concentration and viability using dual fluorescent nuclear stains, for human, rat, mouse and horse. »

Immunology

Immunology Research

Automatically quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, slpenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

PBMC

Peripheral Blood Mononuclear Cells (PBMC)

Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »

Platelets

Platelets

Automatically measure mouse and human platelet concentration using brightfield only method. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Yeast Viability

Yeast Concentration & Viability

Live yeast cell concentration and viability are measured automatically based on the membrane permeability of the fluorescent dye Oxonol. Multiple strains of yeast with viability ranging from 88 to 5% were measured. »

WBSc in Whole Blood

WBCs in Whole Blood

Automatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »

WBSc in Whole Blood

Cell Counting with a Hemacytometer

Learn how to count cells manually with a hemacytometer. Cell viability using trypan blue, yeast viability using methylene blue and crystal violet protocol for total nuclei counting are also covered »

Request a Demo

Request a Demo

Online and on site Cellometer demos and new user training are available.

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Nexcelom Video Library

Demonstrations, webinars, Cellometer features, assay principles and much more.

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Cell Image Gallery

Our cell image gallery with hundreds of cell types...

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Cell Counting Tutorials

A series of mini presentations.

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Product References

View recent Cellometer product references from peer-reviewed publications.

Contact Us

978-327-5340